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Citations for Cell Signaling Technology :
8251 - 8300 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Developmental Biology 2022Quote: ... Coverslips were then incubated overnight in a humidity chamber with the following primary antibodies: β-III-Tubulin (TUJ1, 1:400; Cell Signaling Technology, 5568) and GFAP (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... s6k1 and tor to determine the cross-link between TOR and RPs using anti-human S6K1 antibody raised in mouse (anti phospho70kDa-S6K1-Thr(P)-389) (Cell Signaling Technologies, cat# 9206). The anti-70S6K1 (CST ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and incubation with the primary antibodies in the blocking buffer for overnight at 4°C was followed by a two-hour incubation with secondary antibodies (Cell Signaling, Danvers, MA, USA) in the blocking buffer at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Reactions were analyzed by NU-PAGE Nu-PAGE and immunoblotted with the rabbit monoclonal antibody to CD82 (D7G6H) was used (Cell Signaling Technology Cat# 12439S).
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2019Quote: ... YFP-TbID and BZR1-MH were detected by monoclonal anti-GFP (HT801, Transgen Biotech, Beijing, China) and monoclonal anti-Myc antibodies (9B11, Cell Signaling Technology, Danvers, MA), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently incubated overnight at 4 °C with the primary anti-ADPr-antibody (diluted 1:10’000 in TBS-T; Cell Signaling Technology, Leiden, Netherlands25, 26). Membranes were washed 3x for 5 min with TBS-T and incubated IRDye goat-anti-rabbit IgG secondary antibody (diluted 1:15’000 in TBS-T ...
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bioRxiv - Systems Biology 2020Quote: To probe signaling activity we used the following antibodies: anti-Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb (Cell signaling technology #9624S, 1:2000), anti-Phospho-CREB (Ser133 ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-AMPK antibody (cat no: 2532; used at 1:1000 dilution for western blotting analysis) were obtained from Cell Signaling Technologies (MA, US). Anti-tubulin antibody (cat no ...
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A mean-field approach for modeling the propagation of perturbations in biochemical reaction networksbioRxiv - Pharmacology and Toxicology 2021Quote: ... The c-Myc protein levels in treated cells were measured using intracellular flow cytometry analysis as previously described using an Alexa Fluor 647 conjugated anti-MYC antibody (Cell Signaling Technology, Cat # 13871). Chop protein expression was estimated by Chop reporter expression [28] and Chop mRNA levels ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Neuroscience 2019Quote: Primary antibodies used for immunoblotting were rabbit anti-PKC motif phosphoserine [(R/KXpSX(R/K)] MultiMab mAb mix (1:250; Cell Signaling Technology; Cat# 6967), rabbit anti-Borg4 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... The blots were washed and immunoreactive bands were detected by incubation with anti-rabbit HRP conjugated antibody (dilution 1:2000 in blocking solution; Cell signaling, Cat no. 7074S) for 1h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... was performed for 24 h and cells were subject to immunofluorescence staining with an anti-BrdU antibody (1:400 dilution, Cell Signaling, cat no. 5292) before counterstained with DAPI and assessed by fluorescence microscopy.
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysates (20 µg) were subjected to SDS-PAGE and immunoblotted with specific antibodies against pFRS2a (#3864; Cell signaling Technology, Beverly, MA, USA), FRS2 (sc-17841 ...
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... and a mouse monoclonal antibody against the Ser19 phosphorylated form of the myosin II regulatory light chain (P-MyoRLC) from Cell Signaling Technology (Danvers, MA). Appropriate secondary antibodies conjugated to Alexa Fluor 488 ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Each membrane was washed and primary antibodies (except β-actin-HRP) were detected with a 1:5000 dilution of HRP-conjugated rabbit anti-mouse IgG (Cell Signaling Technology, 7076S) or mouse anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-rat IgG secondary antibody (Cell Signaling Technology, Danvers, MA, USA) for 2 h ...
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bioRxiv - Genetics 2020Quote: ... Cells were filtered and incubated in a 1:500 solution of mouse anti-HA antibody covalently bound to Alexa 647 (Cell Signaling 6E2, product #3444). Cells were incubated with the antibody by shaking vigorously for 15-30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... fixed with 4 % paraformaldehyde/1xPBS for 10 min at RT and solubilized with 0.1% Triton X-100 /1xPBS before staining with antibodies against LC3A/B (Cell Signaling Technologies, cat no. 12741S) and SQSTM1 (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... was performed for 16-18 h and cells were subject to immunofluorescence staining with an anti-BrdU antibody (1:400 dilution, Cell Signaling, cat no. 5292) before counterstained with DAPI and assessed by immunofluorescence and high content analysis microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 5% milk and incubated overnight at 4 °C in primary antibody (anti-PPARγ, Santa Cruz #sc-7273; or anti-β-actin, Cell Signaling #3700). Washes were performed in TBS-T (20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with primary antibody diluted in TBS-T buffer (1:1000 Total OXPHOS Rodent WB Antibody Cocktail [abcam Cat# ab110413] or 1:1000 HSP60 XP Rabbit mAB [Cell Signaling Technology CAT#12165]) overnight at 4°C with gentle rocking ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed with horseradish peroxidase-conjugated antibodies directed against rabbit or mouse IgG (HRP-linked α-mouse IgG (Cell Signaling Technology, Cat. 7076), HRP-linked α-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204, #4370; Cell Signaling Technology, Danvers, MA). Phosphorylation of Hog1 MAPK was detected using rabbit anti-Phospho-p38 MAPK antibody ((Thr180/Tyr182 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were subsequently incubated overnight at 4° C with primary anti-phosphorylated ERK antibody (anti-pERK, Cell Signaling, Cat # 4370, RRID: AB_2315112, 1:400). After primary antibody incubation and PBS wash ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15%) and respectively incubated overnight with primary and corresponding secondary antibodies [rabbit anti p-mTOR (1:1000; #5536, Cell Signaling Technology, Danvers, MA, USA); rabbit anti mTOR (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... the sonicated lysate was then separated to a new tube and primary antibodies was added (anti-H2AK119Ub Cell Signaling D27C, anti-H3K4me3 Cell Signaling CD42D8, anti-H3K4me2 Cell Signaling C64G9) and incubated overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a variety of primary antibodies including anti-MFN2 (Cell Signaling Technology; diluted 1:200), anti-TRA-1-81 (diluted 1:200 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution, or rabbit anti-pMET, #3077, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... CUT&RUN was performed as previously described (Ahanger et al., 2021) with the following antibodies: anti-H3K4me3 (Cell Signaling Technologies, no. 9751, 1:100) and anti-H3K27m3 (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated overnight with the following primary antibodies at 4°C: rabbit anti-Ki67 (1/200; 12202; Cell Signaling Technology, Danvers, MA), rabbit anti-Olfm4 (1/400 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by exposure to primary antibody (anti-Alk1 Rabbit pAb, Abcam #ab68703, 1:500; or, anti-Tubulin Rabbit Ab, Cell Signaling #2144S, 1:500) overnight in 1% BSA TBS-T at 4°C ...
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bioRxiv - Immunology 2024Quote: ... anti-phospho-AMPKα (Thr172) (40H9) (#2535, diluted at 1:1000), anti-AMPKα (#2532, diluted at 1:1000) antibodies from Cell Signaling Technology (Massachusetts, USA), anti-IRF5 (#ab21689 ...
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bioRxiv - Cell Biology 2023Quote: ... The MEL cells were bound to Concanavalin A Beads and Incubated with 1:50 rabbit polyclonal Phospho-Rpb1 CTD Ser5 and Ser2 antibody (Cell Signaling 13523 and 13499). Guinea pig a-rabbit antibody was used at 1:100 dilution as secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies used were p-ser129 alpha-synuclein (FujiFilm cat# 015-25191, 1:1000) and HO-1 (Cell Signaling cat# 26416, 1:200). Sections were allowed to incubate overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were separated via SDS–PAGE and visualized by western blotting with Src antibody (Cell Signaling, #2109 for WT and #2102 for SrcEEI) on Li-Cor Odyssey ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
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bioRxiv - Molecular Biology 2023Quote: ... or 50 ng DPC-DNA (for 6 hpf embryo samples) was subjected to western blotting and immunostained with the anti-H3 antibody (Cell Signaling, #9715, 1:3000). Briefly ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or GAPDH-HRP Conjugate (Cell Signaling ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...