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Citations for Cell Signaling Technology :
7751 - 7800 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody generated in Rabbit (1:2000; Cell Signaling Technology #7074) for 1.5 hours at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... supernatant from the phosphotyrosine IP was incubated with 40 μL of anti-(pS/pT)Q and anti-(pS/pT)P antibody pre-conjugated to agarose beads (Cell Signaling Technologies, catalog #s 12267 and 14378S ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were washed in TBST and incubated for 60 minutes with rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technology 7074) diluted at 1:2000 in 5% milk-TBST ...
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bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween 20 (TBST) for 1 hour at room temperature and then incubated overnight at 4°C with antibodies recognizing human TIM4 (Cell signaling technology, #75484T ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked in 5% non- fat milk or bovine serum albumin (BSA) for 1h and incubated overnight at 4°C with primary antibodies against Cyclin T1 (Cell Signaling Technologies), p24 (Abcam) ...
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bioRxiv - Cell Biology 2024Quote: ... the blots were washed with TBS-T(x3) and incubated with a secondary peroxidaseLconjugated antirabbit or mouse IgG antibody (1:2000) (#7074 or #7076, Cell Signaling Technology) overnight ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA, 1:1000, #5661, Cell Signaling Technology, USA ...
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bioRxiv - Molecular Biology 2024Quote: ... To normalize the phospho (Thr389)-p70 S6 Kinase signals membranes were stripped and reprobed with a phosphorylation-independent anti-p70 S6 Kinase antibody (clone 49D7, Cell Signaling Technology). The expression of the “classical” housekeeping genes β-actin and GAPDH is regulated during myogenesis (Hildyard & Wells ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and 5% non-fat milk for 60 minutes at room temperature and incubated with primary monoclonal antibody (1:1000 rabbit anti-FLAG, anti-HA, Cell Signaling Technologies) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight at 4°C with primary antibody detecting light chain 3 type 1 and 2 (Cell Signaling technology, catalog #3868), SOAT1 (catalog #84476) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The following primary antibodies were used (all rabbit): Vinculin (E1E9V) XP® Rabbit mAb (#13901) and β-actin (#4967S) from Cell Signaling, Danvers ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibodies (1:500 HCS-1 mouse monoclonal, DSHB, University of Iowa; 1:400 anti-cleaved Caspase-3 rabbit polyclonal, Cell Signaling Technology). After overnight incubation in primary ...
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bioRxiv - Immunology 2024Quote: ... then stained at 4°C overnight with various combinations of the following antibodies: rabbit anti-NeuN (1:1000, Cell Signaling Technologies, 12943S), rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody rabbit anti–Calreticulin at 1:100 (Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology for immunoblot or fluorescence imaging analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained according to the manufacturer’s instructions. The antibodies used were directed against phospho-S6 (i.e. activated S6) conjugated to Alexa Fluor 647 (Cell Signaling Technology, 4851). Cells were then analyzed on an LSR IIC flow cytometer (Becton Dickinson) ...
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bioRxiv - Plant Biology 2024Quote: ... After addition of a 1:5000 dilution of horseradish peroxidase-conjugated horse anti-mouse secondary antibodies (Catalog #7076; Cell Signaling Technology, USA), proteins were detected using an ECL kit (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... then probed overnight with our custom rabbit polyclonal anti-Abcg2a antibody (diluted 1:5000) and rabbit monoclonal anti-beta actin (Cell Signaling Technologies, Danvers ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed samples were incubated with primary antibodies diluted in PBS with 1% goat serum and .1% tween-20: YAP (1:200, Cell Signaling, 14074), TAZ (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed 3X with TBST for a total of 15 minutes and incubated with HRP conjugated secondary anti-mouse (7076S) or anti-rabbit (7074S) antibodies at a 1:5000 ratio (Cell Signaling Technology) for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in FACS buffer and used 1:100 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058) and 1:500 for anti-rabbit Alexa-647 (goat ...
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bioRxiv - Developmental Biology 2024Quote: ... They were washed three times with 1x TBST followed by incubation with the HRP-conjugated secondary antibody (1:2000) (Cell Signaling Technology) in 10 ml 1x TBST for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP, 7076; Cell Signaling) for 1 h at room temperature ...
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bioRxiv - Genomics 2024Quote: ... The extent and kinetics of IKAROS1 degradation was determined by western blotting of cell lysates with an IKZF1 antibody (clone D10E5, Cell Signaling Technology).
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bioRxiv - Genetics 2024Quote: ... for 30 min and then incubated with 1:50 diluted anti-cleaved caspase 3 antibody in phosphate-buffered saline (PBS) (Cell Signaling, 9661) for 1 hour ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... mouse monoclonal anti-SARS-CoV-2 Spike clone 2B3E5 (This antibody was used in this study as clone E7U60, which was the pre-production clone ID of clone 2B3E5; Cell Signaling Technology).
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bioRxiv - Immunology 2024Quote: ... The membranes were probed overnight at 4°C with rabbit monoclonal antibodies against inducible nitric oxide synthase (iNOS; Cell Signaling Technology, 2982) or arginase-1 (Arg-1 ...
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bioRxiv - Bioengineering 2024Quote: ... The antibodies against phospho-STAT3 (Tyr705, 9145), cleaved caspase-3 (Asp175, 9579) and PCNA (2586) were purchased from Cell Signaling Technology (USA). The antibodies against myeloperoxidase (ab208670 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were fixed 30 minutes post irradiation with 2% paraformaldehyde for 10 minutes followed by fluorescence immunocytochemistry staining for non-homologous DNA end joining (NHEJ) with incubation with the primary antibodies anti-53BP1 (Cell signaling, 4937) and anti-γH2AX (Cell signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... p-S6S235/236 (#2211; 1:500) and p-4EBP1T37/46 (#2855; 1:1000) antibodies were obtained from Cell Signaling (Danvers, MA, USA). Cre antibody (NB100-56135 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... GST-PpROP4 was detected with an anti-GST primary antibody (26H1) of mouse origin (Cell Signaling Technology, cat. 2624-100µl, 5000× dilution) and an anti-mouse-HRP (goat ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and then incubated overnight at 4°C with anti-p-AKT (Ser473) antibody (1:1000 dilution, RRID: AB_2315049, Cell Signaling Technology 4060T) and anti-H3 antibody (internal control ...
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bioRxiv - Molecular Biology 2023Quote: ... The precleared lysates were transferred to new tubes and mixed with Rabbit anti-KDM2A antibody (Cat # ab191387) or Rabbit IgG control (Cat # 3900S, Cell Signaling Technology) for 4 hr and up to overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated overnight at 4°C with various antibodies: rabbit anti-pan ubiquitin (1:1,000, #3933S; Cell Signaling Technology, Danvers, MA), rabbit anti-K48-linkage specific polyUb (D9D5 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The sections were incubated with primary antibody (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2, 1:1000, Cell Signaling; rabbit anti-AKT3 ...
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bioRxiv - Systems Biology 2023Quote: ... rinsed in wash buffer and incubated for one hour in the primary antibodies (PAK4 Santa Cruz Biotechnology sc-390507 1:75 primary dilution, TGFBI Abcam ab170874 1:300 primary dilution, DDR1 Cell Signaling #5583 primary dilution 1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat. 901514; rabbit, Cell Signaling 3724S), and two PLA probes (anti-rabbit-PLUS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with a rabbit polyclonal anti‐pERK antibody (Cell Signaling; 9101L, lot #30, 1:1000), and mouse anti-NF200 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The blots were subsequently incubated overnight at 4°C with primary antibodies against p53 (DO-7 mouse mAb, Cell Signaling, Cat # 48818) and β actin (13E5 rabbit mAb ...