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Citations for Cell Signaling Technology :
7701 - 7750 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242 ...
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bioRxiv - Immunology 2020Quote: ... Blots were then incubated in HRP-linked secondary antibody against mouse or rabbit IgG (1:3000, Cell Signaling, Cat# 7074S and 7076S) in 4% BSA for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies (diluted in 3% w/v BSA in TBST) were used: adipose triglyceride lipase (ATGL) (1:1000, Cell Signaling #2138), perilipin5 (PLIN5 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
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bioRxiv - Genomics 2020Quote: To assess editing efficiency,1 × 106 cells were taken from culture five days post electroporation and stained with the following primary anti-human antibodies: Cbl-b (Clone D3C12, Cell Signaling Technologies) followed by AlexaFluor 647 F(ab’)2 goat anti rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin immunoprecipitation was performed as described previously (52) 72 hours after transfection with control or RNF40 siRNAs using antibodies against H2Bub1 (Cat. No. 5546S, Cell Signaling Technology) and H3K4me3 (Cat ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... blots were incubated overnight at 4°C in the following primary antibodies: β-III-Tubulin (TUJ1, 1:1000; Cell Signaling Technology, 5568), SUFU (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Cancer Biology 2022Quote: The cells surface expression of MDR1 in parental and resistant Y79 cells was detected using an anti-MDR1 antibody (1:500, cat#13342, Cell Signaling Technology). Parental and resistant Y79 cells post-drug exposure was incubated in 200µl of PBS containing 1% FBS and 2ug of MDR1 antibody at 4°C for 1hour in an intermittent shaker ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed and processed for immunofluorescence as described above with a rabbit ɑ-FLAG antibody (1:1000, Cell Signaling Technologies, D6W5B) to visualize BicD2-KIF fusions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with primary anti-RAB5 antibody at 4 °C overnight diluted in 8% BSA in PBST (1:500 dilution, Cat. #3547, lot 7, Cell Signaling Technology). Secondary anti-rabbit Alexa Fluor 568 antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with antibodies against Vinculin (E1E9V) XP® Rabbit mAb (1:1000; #13901, Cell signaling technologies, Danvers, MA, USA), Sestrin2 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1 h incubation with the HRP-conjugated secondary antibody (Cell Signaling 7076S, 1:10,000 in 2.5% milk in TBS-T). After three washes in TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Biophysics 2022Quote: ... B) Western blot of DMSO and dTAG-13 treated WT HeLa cells and TCOF1 degron cells using HA antibody (Cell Signaling (C29F4), 3724S ...
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bioRxiv - Biophysics 2022Quote: ... 0.1% Tween-20) for 1 hour and blotted with primary antibody (anti-TCOF1, Proteintech, 11003-1-AP; anti-HA, Cell Signaling (C29F4), 3724S ...
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bioRxiv - Biochemistry 2022Quote: ... PDGFRβ was immunoprecipitated from the supernatant with 25 μL of monoclonal anti-HA antibody conjugated to Sepharose beads (Cell Signaling, no. C29F4). KIT and PDGFRβ immunocomplexes were washed three times with washing buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The total p53 and its phosphorylation at Thr55 were detected by anti-p53 antibody (Cell signaling Technology, 1C12, Cat#: 2524, 1:1000) and anti-p53 pThr55 antibody (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Biochemistry 2022Quote: ... Unroofed RBCs were blocked for 30 mins at room temperature in blocking buffer and then incubated with 1:200 rabbit anti-HA primary antibody (3724; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20% swine serum in PBS and then incubated with primary antibodies overnight at 4 °C (γH2AX ((#9718, Cell Signaling Technologies). The following day ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated in an HRP-conjugated secondary antibody (1:1000; Santa Cruz, Dallas, TX, USA or Cell Signaling Technology, Danvers, MA, USA) for 2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run in SDS-PAGE and the mTORC1-mediated phosphorylation on 4E-BP1T37/46 was detected by immunoblotting with a specific antibody (#9459, Cell Signaling Technology). Signals were quantified using the gel analysis tool of ImageJ and shown as phospho-4E-BP1T37/46/4E-BP1 ratio.
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The remainder of the chromatin solution was incubated with Dynabeads previously coupled with 10 μg ChIP grade antibodies (POGZ, Abcam, ab167408;Flag, Sigma, F1804/F3165; H3K4me3, Abcam, ab1012; H3K27ac, Millipore, MABE647; H3K9me3, Abcam, ab176916; H4K20me3, Cell Signaling Technology, 5737s ...
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bioRxiv - Synthetic Biology 2022Quote: ... monoclonal population were expanded and screened for the activation of the GFP ligand after stimulation with anti-myc antibodies (Cell Signaling Technology) bound to an A/G plate (Thermo Scientific).
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bioRxiv - Plant Biology 2022Quote: ... Protein electrophoresis and Western-Blot were performed as described previously using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 8 min at 37 °C with primary antibodies against HA (rabbit, 1:250, Cell signaling Technology, cat #C29F4). After washes ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Physiology 2023Quote: ... Beads were washed in lysis buffer and immunoblotting was done as described above using a PKA substrate monoclonal antibody (Cat#9624, Cell Signaling Technology).
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bioRxiv - Microbiology 2022Quote: ... membranes were washed with TBST and incubated for 1 hour at room temperature with anti-β-Actin antibody (Cell Signaling Technology #3700) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Microbiology 2022Quote: ... Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T). The negative control sample used ΔKu80 parasites (the parental line ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed as described above for c-Fos antibody immunohistochemical staining (c-Fos Rabbit mAb #2250, 1:2000, Cell Signaling Technologies). Brain sections mounted on the slides were scanned using Zeiss Axio Scan.Z1 with 5x magnification ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Molecular Biology 2022Quote: ... Dry peptides were then resuspended in an IAP buffer (Cell Signalling #59322) and incubated with anti-diGly antibody-conjugated slurry (Cell Signaling #59322) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-phospho-p70 S6 kinase (Thr389) (clone 108D2, #9234) and anti-LC3A/B (clone D3U4C, #12741) were rabbit monoclonal antibodies from Cell Signaling Technology. Anti-Akt (#9272 ...
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bioRxiv - Immunology 2022Quote: ... Pellets were then washed and stained intranuclearly with antibodies against NF-kB p65 pS529 AF647 (Clone K10-895.12.50, Cell Signaling Technology, Danvers, MA) (for 30 minutes stimulation samples ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... the sonicated lysate was then separated to a new tube and primary antibodies was added (anti-H2AK119Ub Cell Signaling D27C, anti-H3K4me3 Cell Signaling CD42D8 ...
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bioRxiv - Biophysics 2022Quote: SnapTag and HaloTag Janelia Fluor dyes were obtained from the Janelia Research Campus.54 Rabbit monoclonal antibodies against EEA1 and against LAMP1 were from Cell Signaling Technologies (Catalog # ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in a rabbit monoclonal primary antibody against cFos (1:10,000, 9F6 Rabbit mAb #2250, Cell Signaling, Danvers, MA, USA) in 0.4% Triton-X 100 for 48 hr at room temperature ...