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Citations for Cell Signaling Technology :
7801 - 7850 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... with HRP-conjugated secondary antibodies (Goat Anti-Rabbit Immunoglobulins/HRP, Dako P0448; Horse Anti-Mouse IgG, HRP-linked, Cell Signaling Technology #7076). BCIP/NBT substrate was used to detect ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or Phospho-CD19 (Tyr531 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the western blot was carried out (Odyssey® system) with Anti-Flag (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling). Beta-actin (PA1-183 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed in TBST and then incubated for 45 min at room temperature in blocking buffer containing either anti-rabbit or anti-mouse HRP-linked secondary antibody (Cell Signaling Technology). Membranes were washed in TBST and imaged on the ChemiDoc Imaging System using SuperSignal chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
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bioRxiv - Genetics 2022Quote: ... followed by 1 h incubation in horseradish peroxidase–conjugated secondary antibody (anti-rabbit or anti-mouse, #7074 and #7076, Cell Signaling Technology) diluted in PBS-T 5% milk at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... over night at 4 °C with a dilution of 1:200 and subsequent incubation with a HRP-linked secondary anti-mouse antibody (Cell Signaling #7076) for 2h at RT with a 1:5000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... The pSrc/Src ratio was assessed with a rabbit Phospho-Src Family (Tyr416) Antibody (#2101, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_331697), recognizing the phosphorylation at tyrosine 424 in murine Src ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and myc- and HA-tagged proteins were immunoprecipitated with Protein A/G beads (GeneBio, 22202B-1) coated with anti-myc antibody (Cell signaling, 2276S) at a dilution of 1:250 or anti-HA antibody (Cell signaling ...
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bioRxiv - Immunology 2023Quote: ... A 1:100 dilution of primary antibody (anti-UTX Cell Signaling Rabbit mAb #33510 or IgG Isotype Control: Cell Signaling Technology #3900S) in antibody buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... and XPO1 and Keap1 proteins were detected via western blot using antibodies against XPO-1/CRM1 (D6V7N, rabbit mAb) andKEAP1 (D1G10) Rabbit mAb (both Cell Signaling Technologies). For competition experiments ...
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bioRxiv - Bioengineering 2023Quote: ... Cell lysates were analysed by western blot with anti-Gli1 (L42B10) mouse monoclonal antibody (1:800, 2643, Cell Signaling Technology, RRID: AB_2294746) followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with the appropriate secondary HRP-linked antibody as follows: anti rabbit-HRP (1:3000, Cell Signaling Technology 7074) for NEMO ...
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bioRxiv - Immunology 2023Quote: ... and the transduction efficiency of CAR (% CAR+) was measured by flow cytometry using a PE-anti G4S antibody (Cell Signaling Technology #38907S). T-cells were expanded ...
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bioRxiv - Pathology 2023Quote: ... They were then incubated first with blocking solution for 1 hr and then with a rabbit polyclonal anti-RelA antibody (8242; Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... TritonX-100 in PBS for 1 h at room temperature and then incubated with primary antibody p-STAT-1 (Tyr701) (1:500; Cell Signaling Technology), or CD3χ (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then stained for 1 hour at room temperature with an anti-sheep Alexa Fluor secondary antibody (Cell Signaling Technologies). The entire well was then imaged at 4X magnification using the IncuCyte S3 automated microscope (Sartorius) ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-α-tubulin DM1α conjugated to Alexa Fluor 488 (1:100, Cell Signaling Technologies 8058S), mouse anti-centrin clone 20H5 (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 immunohistochemistry was performed on 4-μm FFPE tumor tissue sections using a standard PD-L1 antibody (E1L3N; dilution 1:100, Cell Signaling Technologies) validated in the clinical laboratory at the study institution ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked using Intercept Blocking Buffer and incubated overnight in the following primary antibodies: rabbit GAPDH (2118S) from Cell Signaling Technology; rabbit BLOC1S1 (19687-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding secondary antibodies conjugated with horseradish peroxidase (cat no: 7074 Rabbit, cat no: 7076 Mouse, cat no: 7077 Rat, Cell Signaling Technology) in combination with enhanced chemiluminescence reagent (cat no ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) or 5% fat-free milk and incubated overnight with primary anti-LEF1 antibody (Cell Signaling, #2230), anti-p16 antibody (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice in 1 ml FACS buffer and resuspended in 50-100 μl of 1:50 diluted primary PE-conjugated antibodies against phosphorylated p38 or Erk1/2 (Cell Signaling Technology) and stained for 1 h at room temperature on a rotor ...
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bioRxiv - Immunology 2023Quote: ... blocked with 5% fat-free milk in TBST and probed with a 1:1000 dilution of an antibody that recognizes the doubly-phosphorylated TGY motif of PMK-1 (Cell Signaling Technology) or a monoclonal anti-tubulin antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 10% non-fat milk in Phosphate-buffered saline with 0.1% Tween-20 (PBST) and probed with antibodies against IFITM1 (Cell Signaling Technology, #13126), IFITM2 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were incubated overnight at 4°C with the primary antibody against VE-Cad (rabbit mAb, D87F2, Cell Signaling Technology, 1:200) diluted in blocking solution ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... Protein lysates containing 1 mg total protein were precleaned and precipitated with indicated antibody or IgG (Cell Signaling Technology, Danvers, MA, US) for 6 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195 ...
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bioRxiv - Developmental Biology 2023Quote: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac ...
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bioRxiv - Developmental Biology 2023Quote: ... In some groups hRGCs Brn3b-tdT were pre-treated with anti-DCC antibody to disrupt Netrin-DCC signaling or rapamycin (Cell signaling; 100nM) was added to the medium to inhibit mTOR signaling.
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bioRxiv - Neuroscience 2023Quote: ... were from Thermo Fisher Scientific (Waltham, MA). Rabbit monoclonal antibody to Flotillin-1 (cat. 3253) and Alix (cat. 2171) were from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody to CD31 (cat ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Physiology 2023Quote: ... for 1 hour and then incubated overnight at 4°C with primary antibodies p-S6 (Ser240/244, 1:1000, Cell Signaling, #2215), S6 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from FT282 and KURAMOCHI were bound to Concalavin A beads and incubated overnight at 4℃ with primary antibody for H3K27me3 (Cell Signaling, RRID:AB_2798370), H3K27ac (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were blocked in 0.5% BR (Blocking Reagent (Akoya Biociences))/TNT (TN, 0.05% Tween) for 1h at RT and incubated with a primary antibody against pSMAD1,5,9 (Cell Signaling 13820S, rabbit, 1:300) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against mouse anti-HA clone 6E2 (Cell Signaling Technology) or rabbit anti-TgSKP1 UOK75 [19].
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bioRxiv - Biochemistry 2023Quote: ... The blots were then incubated with a peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody (1:2000) (# 7074 or #7076, respectively; Cell Signaling Technology). The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003, RRID: AB_2337913], H3K27ac [1:66; Cell Signaling Technology, 4353S ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with BSA 1% for at least 3h, cells were incubated with two primary antibodies RIP140 (sc-9459, Santa-Cruz) and HES1 (#11988, Cell Signaling Technology) overnight at 4°C ...