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Citations for Cell Signaling Technology :
7851 - 7900 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against mouse anti-HA clone 6E2 (Cell Signaling Technology) or rabbit anti-TgSKP1 UOK75 [19].
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bioRxiv - Biochemistry 2023Quote: ... The blots were then incubated with a peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody (1:2000) (# 7074 or #7076, respectively; Cell Signaling Technology). The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003, RRID: AB_2337913], H3K27ac [1:66; Cell Signaling Technology, 4353S ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with BSA 1% for at least 3h, cells were incubated with two primary antibodies RIP140 (sc-9459, Santa-Cruz) and HES1 (#11988, Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies (γH2AX 1:300, Cell Signaling Technology, Cat#2577; RPA 1:300, Millipore, Cat#MABE285; FOXM1pT600 1:1000, Cell Signaling Technology, Cat#14655 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Neuroscience 2023Quote: ... Double labeling for phospho-c-Jun and TH was performed with phospho-specific rabbit monoclonal antibody (Cell Signaling, cat.# 3270; 1:500) for c-Jun and sheep anti-TH antibody (Novex Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... c-Jun phosphorylated at the JNK phosphorylation site Ser73 was detected with phospho-specific rabbit antibody (Cell Signaling, cat.# 3270; 1:1,000) and the total c-Jun – with mouse monoclonal antibody (Cell Signaling ...
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bioRxiv - Physiology 2023Quote: ... 1:1000 dilution), antianti-p-MOB1 (#8699, 1:1000 dilution), and anti-MOB1 (#13730, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. Anti-phospho-Mst1 (AP0906 ...
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bioRxiv - Physiology 2023Quote: Immunoblot experiments were performed as previously described (17, 18) using antibodies against cleaved caspase-3 (Cell Signaling Technology, Cat#9664, RRID: AB_2070042), procaspase-3 (Cell Signaling Technology ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... the blots were washed with TBS-T(x3) and incubated with a secondary peroxidaseLconjugated antirabbit or mouse IgG antibody (1:2000) (#7074 or #7076, Cell Signaling Technology) overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were washed in TBST and incubated for 60 minutes with rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technology 7074) diluted at 1:2000 in 5% milk-TBST ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA, 1:1000, #5661, Cell Signaling Technology, USA ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were incubated overnight at 4°C with a 1:100 dilution of phospho-Stat3-Tyr705 antibody (cat#9145, Cell Signaling Technology) in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated for 1 h at room temperature with secondary goat anti-rabbit horseradish peroxidase antibody (Cell Signaling Technology) at 1:10,000 in 5% BSA-TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 1ml total volume cold Co-IP Buffer containing rabbit IgG or rabbit anti-FLAG antibody (Cell Signaling #14793S), 4μg per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... The membranes were incubated overnight at 4 °C with primary antibodies against VDAC1 and β-Actin (1:5000, #2146; Cell Signaling Technology) after the washing step with secondary antibodies HRP-conjugate Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% milk in 1X TTBS and incubated overnight with the appropriate primary antibody (Cell Signaling c-IAP2 58C7 Rabbit mAb #3130; Cell Signaling XIAP Antibody #2042 ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lack of TLR2 expression in the negative population was confirmed by western blot using a different antibody (Cell Signaling Technology-CST, 13744). Re-expression of mTLR2 or its mutants was achieved by transduction of negative cells with retrovirus carrying the relevant constructs and repeated sorting of the positive population using the aforementioned method ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was performed for 1h at room temperature in phospho-p44/42 MAPK (ERK1/2) (Thr202/Thr304) antibody (#4370, 1:200, Cell Signaling Technology) or human VE-Cadherin antibody (#AF938 ...
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bioRxiv - Molecular Biology 2023Quote: The following antibodies were used in this study: mouse monoclonal anti-β-actin (Cell Signaling Technology; Cat# 3700; RRID: AB_2242334; LOT# 20), rabbit polyclonal anti-FLAG (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2024Quote: ... The following primary antibodies were used (all rabbit): Vinculin (E1E9V) XP® Rabbit mAb (#13901) and β-actin (#4967S) from Cell Signaling, Danvers ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked in 5% non- fat milk or bovine serum albumin (BSA) for 1h and incubated overnight at 4°C with primary antibodies against Cyclin T1 (Cell Signaling Technologies), p24 (Abcam) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The mouse anti-rabbit (#3678S) and the anti-mouse HRP-conjugated secondary antibodies (#7076S) were from Cell Signaling Technology (Beverly, MA, USA). Enhanced chemiluminescence was used for detection (Amershan Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 45 min with 3 μg/ml Myc-Tag (9B11) Mouse mAb AlexaFluor 488 conjugate antibody (Cell Signaling Technology) and Phalloidin-Alexa 647 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... at a 1:3000 dilution in TBS-T and rabbit polyclonal anti-Phospho-p70 S6K(Thr389) antibodies (#9205, Cell Signaling Technology, USA) at a 1:1000 dilution in TBS-T ...
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bioRxiv - Genomics 2024Quote: The CD34+ cells were treated with TAK-243 for 48 h and the cells were collected and immobilized on magnetic beads conjugated with a specific antibody against ATF4 (Cell signaling; (D4B8) Rabbit mAb #11815 ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Systems Biology 2024Quote: ... Primary antibodies (FOXP1 Cell Signaling Technologies #2005S, FOXP2 Abcam #ab16046, FOXP4 Millipore #ABE74, H3K27me3 Cell Signaling Technologies #9733, IgG Epicypher #13-0042) were added at 1:50 dilution and samples were nutated overnight at 4C.
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of SARS-CoV-2 nucleocapsid protein (NP) (E8R1L) fragmented mouse monoclonal antibody f-mAb (Cell Signaling Technology Inc., Massachusetts, USA) are introduced to the sensor surface after diluting in PBS and incubated for 2 hours at room temperature ...
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bioRxiv - Genetics 2024Quote: ... Cells were treated and prepared as described above for synchronization and flow cytometry with the addition of cleaved caspase-3 antibody (Cell Signaling Technology) conjugated to Alexa Fluor 647 added together with pH3 antibody ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight with primary antibody (1:1000 diluted in blocking serum, monoclonal anti-rabbit DCX; Cat#4604S, Cell Signaling Technology) on a shaker (Model# BDRAA115S ...
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bioRxiv - Cell Biology 2024Quote: ... The required primary antibody (JOSD1 [Abcam, Cambridge, United Kingdom], SOCS1 [Abclonal, Woburn, Massachusetts, United States of America], Cleaved Caspase 3 [Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [HRP-conjugated anti-mouse IgG (1:5000; Cell Signaling Technology; #7076) and HRP-conjugated anti-rabbit IgG (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated overnight at 4 °C with antibodies specific to phospho-histone H3 (Ser10) at a dilution of 1:100 (Cell Signaling Technology). After the primary antibody incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... The coverslips were then incubated with primary antibodies against BioID2 (1:5000, BioFront #BID2-CP-100) or HA tag (1:800, Cell Signaling #3724) diluted in 1% BSA/ PBS-Tween 0.1% for one hour in a humidified chamber at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then washed with 1X PBS (2 times, 5 minutes each) and incubated with the primary antibody (COL1A1, Cell signaling #72026S) at 1:50 dilution for 24h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized with ice cold 100% methanol to a final concentration of 90% with gentle vortexing and stained using AlexaFluor (AF) 488-conjugated Ki-67 monoclonal antibody or AF488-conjugated IgG as an isotype control (Cell Signaling Technologies). Data were acquired from ∼1×104 cells using a BD LSR II Flow Cytometer (BD Biosciences) ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with the primary antibodies (mouse anti-firefly luciferase, clone Luci17; ab16466, Abcam; rabbit monoclonal anti-mouse CD8, 989415, Cell Signaling Technology; rabbit anti-NK1.1/CD161 ...
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bioRxiv - Physiology 2024Quote: ... which was blocked using Odyssey blocking buffer (Li-Cor, USA). Membranes were incubated with primary antibodies (GAPDH, Cell Signaling, Cat. 97166 [D4C6R], mouse mAb, 1:1000; (A-Tubulin, Cell Signaling, Cat ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in TBST plus 5% non-fat milk and then incubated with the following primary antibodies: phospho-S6 (S235/236; 1:2000, Cell Signaling, #4858), S6 (1:2000 ...