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Citations for Cell Signaling Technology :
7551 - 7600 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Immunology 2020Quote: ... p-Akt (Ser473) (Clone D9E) and total Akt (clone 4604) and c-Myc antibody (Clone 9E10) were obtained from Cell Signaling Technology. Alfa-tubulin antibody was purchased from Abcam (Clone DM1A) ...
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bioRxiv - Biochemistry 2019Quote: ... After washing with TBST blots were then incubated with a 1:1000 dilution of the primary antibody (p-CRAF (S259) (Cell Signaling Technology), p-CRAF (S298/296 ...
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bioRxiv - Cancer Biology 2020Quote: ... and rabbit monoclonal vinculin antibody (clone E1E9V; #13901, 1:1000; epitope: amino terminus of human vinculin protein) were purchased from Cell Signaling Technology.
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bioRxiv - Cell Biology 2019Quote: Western blots for quantifying the Twitch-2B concentration were probed with a primary antibody against GFP (Cell Signaling Technology, Beverly, MA; #2555); this antibody also recognizes CFP and YFP ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal SDHA (#11998, 1:1000) and rabbit monoclonal COXIV (3E11 clone, #4850, 1:1000) antibodies were purchased from Cell Signaling Technology. The donkey anti-rabbit (NA934 ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed an additional three times for five minutes each before incubating in a 1:2000 dilution of anti-mouse horseradish peroxidase conjugated antibody (Cell Signaling Technologies) in TBST ...
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bioRxiv - Biophysics 2019Quote: ... We also probed for the presence of eRF1 using Western blotting after the ribosome pelleting step (anti-eRF1 antibody from Cell Signaling Technology).
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were blocked in 5% milk/PBS-T buffer for 30 min and incubated either overnight at 4°C or for 1 hour at room temperature with the following antibodies: Chd1 (Cell Signaling, 4351), Top2β (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2019Quote: ... Sheared chromatin was then incubated with primary antibodies (AR [06-680, Millipore], H3K4me3 [ab8580, Abcam], phospho-Ser5 RNA polymerase 2 [ab5131, Abcam], RNA polymerase 2 [4H8, Cell Signaling Technologies] or control IgG [Cell Signaling Technologies] ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Systems Biology 2020Quote: ... we washed the cells 5 × 5 minutes with 5% BSA-PBS then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 donkey anti-mouse secondary antibody conjugated to Cy3 (Jackson 715-165-150) and 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling #8889). After the secondary incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody was added to each tube (10μL anti-Histone H3, Cell Signaling Technology, cat #4620; 4μg Normal Rabbit IgG, Cell Signaling Technology, cat #2729 ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Plant Biology 2020Quote: ... MAPK phosphorylation was detected via immunoblotting using an antiphospho-p44/42 MAPK antibody (dilution 1:5000 in TBST, Cell Signaling Technology, USA) as primary and HRP-conjugated anti-rabbit IgG (1:10000 in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Gapdh was used as a loading control and was detected using a rabbit monoclonal anti-Gapdh antibody (1:2000, Cell Signaling Technology).
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl permeabilization medium with 1:100 diluted Alexa Fluor 647-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology) and incubated for 30 min in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight in primary antibodies (EZH2; Cell Signalling; Beta Actin, ProteinTech; ARL13B, ProteinTech; IMPDH2, Abcam; Oct4, Cell Signaling; Sox2, Cell Signaling; Shh ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in PBS and incubated for 1h with A488-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling, USA). Similar procedures were followed for POMC labelling in brain sections from POMC-CreERT2-Ai6 mice ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked with 5% milk in 5% tris-buffered saline with 0.01% Tween-20 (TBST) for 1 hour at room temperature then incubated overnight with the primary antibodies anti-GAPDH (Cell Signaling Technology) and anti-IVNS1ABP (Atlas Antibodies) ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then washed 3x with TBST at room temperature then incubated with secondary anti-rabbit HRP-conjugated antibody (Cell Signaling Technology) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in a rabbit anti-HA monoclonal antibody (1:250 in blocking buffer; catalog no. 3724, Cell Signaling, Danvers, MA) for 48 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Plant Biology 2020Quote: ... Phosphorylated MAPKs were detected using α-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) rabbit monoclonal antibody (1:2000, Cell Signaling Technology, USA) for 1 h at room temperature in a solution of 5 % (w/v ...
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bioRxiv - Bioengineering 2021Quote: ... Protein level could also be measured by immunofluorescence using fluorescently labeled antibodies (anti-Flag-Alexa 647, #15009; Cell Signaling, Danvers, MA, USA). These antibodies were flowed into the device and incubated with the immobilized proteins under the ‘button’ for 20 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Flare Biotech LLC) following by HRP conjugated goat anti-rabbit antibody (1:10,000 Ab in 3 % BSA in TBST; Cell Signaling Technology Inc). Images obtained were analyzed via ImageJ software.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by a 1 h incubation in the HRP-labelled secondary antibodies (horse anti-mouse IgG (Cell Signaling Technology, Cat. No: 7076), and goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in skim milk or BSA and probed overnight with the following antibodies: Anti-AML1 (Cell Signaling Technology, Cat# 4336); anti-acetyl-histone H3 (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.8mg total protein for each sample was digested with trypsin and purified over C18 columns for enrichment with the Symmetric Di-Methyl Arginine Motif Antibody (Cell Signaling #13563). Enriched peptides were purified over C18 STAGE tips46 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitated proteins were eluted from the beads using 150 µl of 1x Laemmli sample buffer for 5 min at +75□C and analysed by western blotting as described above except the HRP-conjugated mouse anti-rabbit native IgG secondary antibody (Cell Signaling, #5127) was used to prevent visualisation of heavy and light chains of co-eluted antibody.
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bioRxiv - Cell Biology 2021Quote: ... HA-Tag antibody (C29F4) Rabbit mAb (cat#3724) and Myc-Tag (71D10) Rabbit mAb (cat#2278) all were purchased from Cell Signaling Technology. Anti-ubiquitin (FK2 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4 % paraformaldehyde and 0.2 % picric acid in phosphate buffer (pH 6.9) for 30 minutes at room temperature and incubated with peroxidase-conjugated anti-rabbit antibody (Cell Signaling technology #7074) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated with the secondary antibody for 1h at room temperature (rabbit-IgG HRP-linked 1:10000, Cell Signaling Technology (#7074), mouse-IgG HRP-linked 1:10000 ...
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bioRxiv - Biophysics 2022Quote: ... Cellular protein tyrosine phosphorylation was performed by immunoblotting of total cell lysate (20 μg) using anti-pTyr antibody (4G10, a gift form Prof. Mangeat; CRBM) and anti-Actin (Cell Signaling Technology). Signal quantification and analyses were performed using ImageJ and GraphPad Prism respectively ...