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Citations for Cell Signaling Technology :
8001 - 8050 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... transferred onto nitrocellulose membranes and detected by immunoblot using the following primary antibodies: mGlu5 (rabbit clone D6E7B from Cell Signaling, dilution 1:1000), actin (mouse clone from DSHB ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500; rabbit anti-cleaved caspase-3, Cell Signaling, 1:500) overnight in 3 % BSA in PBS at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Pathology 2021Quote: ... and incubated with primary antibodies at 4°C overnight at the following concentrations: β-catenin (Cell Signaling, 8480, 1:1000 in 1% BSA), p65 (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies for western blots were diluted as follows: rabbit anti-Phospho-AKT Ser473 (D9E) (Cell Signaling Technology, USA; RRID: AB_2797780; 1:2000), mouse anti-Pan-AKT (40D4 ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling Technology, Cat# 12040) or 5ug Normal Rabbit IgG Polyclonal Antibody (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A were washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling TECHNOLOGY, Cat# 12040) or 5ug Rabbit polyclonal anti-phospho-Upf1 (Ser1127 ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were boiled with SDS loading buffer and then resolved with SDS-PAGE and subjected to Western blot analysis using anti-MeCP2 antibody (Cell Signaling Technology, 3456). To quantify the level of O-GlcNAcylation ...
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bioRxiv - Neuroscience 2021Quote: ... the retinal explants were immuno-stained against microtubule with beta-3 tubulin (TUBB3) antibody (1:250, from rabbit, Cell Signaling Technology, 5568S, USA), washed in 0.5% Triton in PBS for 5 times ...
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bioRxiv - Neuroscience 2020Quote: ... Western Blot was performed using an anti-Scn2a antibody (Alomone, Jerusalem, Israel) and a β-actin control (Cell Signaling Technology, Danvers, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were washed with 1% BSA-PBS and incubated (150.000 cells per sample) with pIRF3 primary antibody (1:100, Cell signaling, #29047, clone D601M) for 1 hour at 4 °C and subsequently stained with AlexaFluor 488-conjugated goat anti-rabbit secondary antibody (1:300 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Biophysics 2021Quote: The phosphorylation of proteins was quantified by western blotting using antibodies against pEGFR (#4407 for pY1173 and #3777 for pY1068; Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... the goat anti-mouse (polyclonal 7076) and goat anti-rabbit (polyclonal 7074) peroxidase-conjugated secondary antibodies were from Cell Signaling (Beverly, MA. USA). DMEM complete medium ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked for 1h with 5% BSA in PBS and then stained with rabbit anti-pPLCγ1 antibody (Cell Signaling, #2821, 1/100) overnight at 4C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody binding was directed against eGFP using a solution containing a 1/4000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, USA). For all VLPs ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... blocked with 5% non-fat powdered milk in TTBS solution and incubated with primary antibodies from Cell Signaling (1:1000; Danvers, MA, USA) against p-IGF1RβY1135 (3918) ...
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bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat): anti-Pan-AKT (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Histological sections (5 mm) were stained for immunohistochemistry using a standard protocol with antibodies for LC3A/B (1:200; #12741S; Cell Signaling Technology, USA).
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against pSTAT3 and pAKT were detected by using a conformation-specific HRP-conjugated anti-rabbit IgG secondary antibody (1:4,000; Cell Signaling Technologies; RRID: AB_10892860). Antigen-antibody complexes were visualized by using enhanced chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein of interest was detected using an appropriate antibody specific for phospho-EGFR (Tyr1068) (2234; Cell Signaling Technology [CST], Danvers, MA, USA), EGFR (2232 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000; Rybp Cell Signaling D8J7W 1:1000; SUZ12 Cell Signaling (D39F6) 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100; Mouse Tubulin 1:1000; Cell Signaling DM1A). Primary incubation was followed with HRP-conjugated secondary antibodies (Rabbit-HRP 1:10000 ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Molecular Biology 2019Quote: ... UK with catalogue numbers ab52908 and ab76227 respectively and housekeeping mouse raised β-tubulin (51 kDa) primary antibody was procured from Cell Signaling Technologies, MA ...
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bioRxiv - Molecular Biology 2020Quote: ... The clear supernatant was separated on SDS-PAGE and transferred to PVDF membrane and probed with polyclonal phosphor-Ser/Thr epitope antibodies (Cell Signaling Technology, USA) as described earlier (Rajpurohit et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated and on 10% SDS-PAGE gel and blotted onto PVDF membrane and probed with polyclonal phospho-Ser/Thr antibodies (Cell Signaling Technology, USA). Signals were detected using anti-rabbit IgG conjugated with alkaline phosphatase and the color reaction substrates NBT-BCIP (Roche Biochemicals ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Microbiology 2020Quote: ... Arf6 excision in endothelial cells was verified by isolating lung endothelial cells as previously described (28) and performing a Western blot to assess ARF6 levels using anti-ARF6 antibody from Cell Signaling Technology (#3546) (S5 Figure B).
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bioRxiv - Cancer Biology 2019Quote: ... The immunohistochemical staining was also conducted by incubating of indicated sections with distinct primary antibodies against human Nrf1 (from Cell Signaling Technology, Inc.), β-catenin and Cyclin D1(both obtained from Epitomics ...
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bioRxiv - Cancer Biology 2019Quote: ... 80 μg of total cell lysates were analyzed by Western blot using the following antibodies and concentrations: LRP6 (1:1000, Cell Signaling Technology 3395), LRP5 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53, SC-126, Santa Cruz Biotechnology; rabbit -CTCF, Millipore 07-729; rabbit -GAPDH, cst-2118, Cell Signaling Technology) followed by detection with HRP conjugated secondary antibodies (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Genetics 2021Quote: ... Membranes were rinsed with TBST for 30 min before incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling; 7074S, 1:5000) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Co-IP was carried out in the same way above using NE-4C and HepG2 cells and an anti-PRMT1/Prmt1 antibody (Cell Signaling Technology, #2449). Precipitated proteins were subjected to SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed 1× TBST three times for 10 min each followed by incubation with anti-rabbit IgG horseradish-peroxidase secondary antibody conjugate (1:3000 dilution; Cell Signaling Technology, 7074S) in 1× TBS for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bound beads were boiled with 1X SDS loading buffer and resolved in 10% SDS-PAGE and analyzed by with 1:1000 dilution of rabbit anti-PAX9 polyclonal antibody (Cell Signaling Technology, USA) or 1:1000 dilution of mouse anti-FLAGM2 monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Co-immunoprecipitation of endogenous PARP1 and SET8 were performed with 200 µg of total extract from crosslinked (1% formaldehyde for 10 min) HCT116 or HeLa cells using anti-PARP1 antibody (Cell Signaling Technology # 9532), anti-SET8 antibody (Santa Cruz Biotechnology # sc-515433 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000, Cell Signaling; GCN5L1 1:500 ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... and a 1:1000 dilution of anti-phospho-histone H3 (pHH3) Alexa 488 (Ser10, clone D2C8) conjugated antibody (Cell Signaling Technologies, Danvers, MA). Fixed cells were imaged with a 10x objective using an IXM-C microscope and analyzed using MetaXpress software (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... 15 mg protein from each sample was digested with trypsin and purified over C18 columns for enrichment using phosphotyrosine pY-1000 motif antibodies #8803 (Cell Signaling, Danvers, Mass). Enriched peptides were purified over C18 STAGE tips (16 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was blotted with 5% nonfat milk in TBST at RT for 1h and then incubated with the following antibodies at 4°C overnight: TWIST1(Cell Signaling Technology, 46702S), E-cadherin (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2020Quote: ... using heat-induced antigen retrieval at pH 9.0 for 20 minutes, followed by primary antibody (PD-1, clone EH33; dilution 1:200) (Cell Signaling, Beverly, MA, USA) incubation for 15 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Protein lysates from 28 hpf control and ncl-/- zebrafish were used (n = 25 per biological replicate; total three biological replicates) for pre-conjugation with Mdm2 antibody (Cell Signaling Technology, #86934) and IgG with magnetic beads ...