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Citations for Cell Signaling Technology :
7601 - 7650 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were saturated and blots were probed with the following primary antibodies: 1:700 rabbit anti-βArr2 (Cell signaling Cat. No. 3857); 1:1000 rabbit anti-GSK3β (Cell signaling Cat ...
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bioRxiv - Cell Biology 2022Quote: Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-Phospho-JNK polyclonal antibody (Cell Signaling Technology, Cat #4668, 1:100), chicken anti-β-galactosidase antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed three times with PBS and incubated with a horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (Cell Signaling #7074) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... except cells were cross-linked with formaldehyde (1%) for 5 min and cross-linked chromatin immunoprecipitated using an anti-HA rabbit monoclonal antibody (Cell Signaling Technology). Knock-in ESCs were first adapted to serum-free 2i/LIF condition as described before ChIP (15) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then rinsed three times with PBS before adding 100 ng/mL DAPI and fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:500 in PBS containing 1% BSA and 0.3% Triton-X for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 minutes each at room temperature with agitation and then incubated with Anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling 7076S) in 2% milk powder in PBST and incubated at room temperature for 2 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... The following secondary antibodies and their respective dilutions were used: Anti-mouse IgG(H+L) DyLight 800 conjugate (Cell Signaling Technologies, #5257), 1:20000 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 100 µl/well of diluted H3K9ac on a shaker at 4°C overnight and then treated with Alexa Fluor 488 conjugate secondary antibody (Cell Signaling #4412) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed with tris-buffered saline with Tween 20 (TBST) three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and horseradish peroxidase-conjugated (HRP) anti-mouse and anti-rabbit secondary antibodies were all obtained from Cell Signaling Technologies (Beverly, MA, USA). Mini-protean precast Tris-Glycine Gels (TGX ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed for 10 minutes thrice with 1×TBST before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Cancer Biology 2022Quote: The following antibodies were used for immunoblotting (IB) at 1:1,000 unless otherwise stated: Anti-AXL (8661S, Cell Signaling, IHC 1:500), anti-phospho AXL y702 (5724 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with the following primary antibodies: phospho-p44/42 MAPK (EER1/2) (T202/Y204) (Cell Signaling Technology, Cat. #: 9101, 1:1000), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were blocked with 5% goat serum in tris-buffered saline and 0.1% Tween® 20 detergent (TBST) for 30 min and stained with anti-HER2 antibodies at 1:500 dilution (Cell Signaling #21650). After three washes with TBST ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase inhibition and blocking with normal horse serum was also carried out before the incubation with primary antibodies (anti-rabbit caspase3, 1:100, Cell signaling #9662), anti-mouse ki67 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of puromycin for 48 hours and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of neomycin for 48 hours and then used for HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure MyD88 and TRIF expression using a rabbit monoclonal antibody to MyD88 (Cell Signaling Technology), a rabbit polyclonal antibody to TRIF (Cell Signaling Technology) ...
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bioRxiv - Molecular Biology 2021Quote: ... Detection was performed with horseradish peroxidase-conjugated secondary antibodies: horse anti-mouse (1:5000; Cell Signaling, USA, Cat. No. Cat. No. 7076S), goat anti-rat (1:5000 ...
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bioRxiv - Developmental Biology 2021Quote: ... A mixture of rabbit anti-pSmad1/5/9 (D5B10) and anti-pSMAD1/5 (41D10) monoclonal antibodies (Cell Signaling, #13820 and #9516, respectively), diluted 1:100 each ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight incubation at 4°C with the following primary antibodies: 4EBP1 (1:1,000, Cell Signaling Technology, Cambridge, UK #9644), MYCN (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a secondary antibody goat anti-rabbit conjugated to a horseradish peroxidase (HRP) (1:500, 7074S Cell Signaling Technology) during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a secondary antibody goat anti-rabbit conjugated to a horseradish peroxidase (HRP) (1:500, 7074S Cell Signaling Technology) during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Microbiology 2020Quote: ... Sections were permeabilized with 0.5 % Triton-X for 30 min at RT and stained over night with primary antibodies (rabbit anti-IFITM1 Cell Signaling 13126 S, 1:500 or rabbit anti-IFITM2 Cell Signaling #13530S ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal anti-SARS-CoV-2 Spike clone 2B3E5 (This antibody was used in this study as clone E7U60, which was the pre-production clone ID of clone 2B3E5; Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed with TBST and incubated for 2h at room temperature with Horseradish peroxidase (HRP)-linked secondary secondary antibody (Cell signaling #7074). Chemiluminescent signal was revealed using SuperSignal™ West Pico PLUS Substrate (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... The reaction mixture was resolved by SDS-PAGE and cMYC translation was checked by Western blot analysis using cMYC antibody (Cat# 5605S, Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... Efficacy of individual FXR1 shRNA construct was checked by Western blot analysis for FXR1 knockdown using FXR1 antibody (Cat#12295, Cell Signaling Technology). The most effective shRNA construct was used for generating FXR1 knockdown stable cell line by selection with puromycin (8 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... slide mounted sections were incubated overnight at room temperature with a rabbit anti-cFos monoclonal primary antibody (9F6, Cell Signaling, 1:8000), diluted in 0.1M phosphate buffer at pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression of γ-H2AX was measured by Western blotting using anti-γ-H2AX (Ser139, antibody # 2577; Cell Signaling Technology, Inc., Danvers, MA) as reported by us previously42,44,47.
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% Tween 20) for 1 h at RT and then incubated with specific antibodies (eIF2α, P-eIF2α (Cat# 9722, 9721S, Cell Signaling™), HA (Cat#901501 ...
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody used in for western-blotting have been previously described (Montagne et al. 2010) or commercially provided for Akt (Cell signaling 4054).
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bioRxiv - Cell Biology 2019Quote: ... For immuno staining the phospho-S6 antibody has been previously described (Romero-Pozuelo et al. 2017) and the phospho-Akt commercially provided (Cell signaling 4054). The cell size calculation have been performed as previously described (Garrido et al ...
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bioRxiv - Developmental Biology 2019Quote: ... The presence of the DNA damage response was determined by using a phospho-specific antibody targeting the phosphorylated consensus target site of ATM and ATR kinases (pS/TQ) (Cell Signaling Technology). This antibody has only been shown to stain a DNA damage response in the germline and was used as previously described (Vermezovic et al. ...
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bioRxiv - Microbiology 2019Quote: ... The inhibition of Akt activity was confirmed by western blot analysis with rabbit antibody against phospho-S473-Akt (#9271, Cell Signaling Technology), rabbit antibody against Akt (pan ...
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bioRxiv - Cell Biology 2019Quote: ... samples were transferred to PVDF membranes and western blotting performed as described previously [37] using a primary antibody for phospho-p38 MAPK (#9215; Cell Signaling Technology). Membranes were re-probed with p38α antibody (#9228 ...
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bioRxiv - Molecular Biology 2020Quote: ... or 5 million cells for histone modifications was sheared with SFX250 Sonifier (Branson) and immunoprecipitated with antibodies targeting H3K27ac (#8173, Cell signaling Technology), H3K4me1 (#5326 ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Molecular Biology 2019Quote: ... stained by ponceau for general quality control and immunoprobed with antibodies as following: Aldolase-A (rabbit polyclonal, 1:1000, Cell Signaling Technology), Aly (rabbit polyclonal41 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Immunofluorescent staining was performed as previously described (Ibrahim et al 2019) and the following antibodies were used: rabbit anti-insulin (C27C9, 1:200, Cell Signaling #3014), mouse anti-insulin (L6B10 ...