Labshake search
Citations for Stemcell Technologies :
4801 - 4850 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... genome-edited iPSCs were plated at a density of 250 cells per well of a 6-well plate in mTeSR1 medium supplemented with 1x CloneR2 reagent (cat.: 100-0691; STEMCELL Technologies, Vancouver, Canada). After 48h ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at a high density of (250kcells/cm2 in 6-well plates) as a single-cell suspension in StemDiff Neural Induction Media with Dual SMAD Inhibition (STEMCELL Technologies cat #08581), supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304) ...
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bioRxiv - Genetics 2024Quote: ... was purchased as a single guide molecule from IDT (Integrated DNA Technologies, IA) and complexed with ArchiTect™ Cas9 nuclease (Stem Cell Technologies) for editing experiments in primary human CD34+ HSPCs.
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bioRxiv - Developmental Biology 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
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Transcriptomic Profiling of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Over TimebioRxiv - Cell Biology 2020Quote: ... hESCs were maintained in culture until 70-80% confluent at which stage StemFlex was replaced with Complete E6 (05946, Stem Cell Technologies, Vancouver, Canada) supplemented with N2 (17502048 ...
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bioRxiv - Microbiology 2021Quote: ... pieces of intestinal resections were washed thoroughly in ice-cold PBS and epithelial crypts were subsequently dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies, Vancouver, BC, Canada) by nutating at 4°C for 30 minutes followed by trituration ...
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bioRxiv - Microbiology 2021Quote: ... The cells were cultured and differentiated using Complete PneumaCult™-Ex plus medium and PneumaCult™-ALI-S Maintenance medium (Stemcell Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 5% CO2 for 14 days and the CFC content was automatedly imaged after 7 and 14 days by using the StemVision imaging system (Stem Cell Technologies, Version 2.0.1.0). Additionally ...
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bioRxiv - Bioengineering 2020Quote: Primary human bronchial epithelial cells (HBECs) were isolated immediately from bronchial brushing into T25 flasks containing Pneumacult™ Ex-Plus Basal Media (Stemcell Technologies, Vancouver Canada) with Pneumacult™ Ex-Plus 50x Supplement ...
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Cell Biology 2021Quote: ... We confirmed our iPSC cell banks have normal copy number at various mutation hotspots using the hPSC Genetic Analysis Kit (Cat # 07550, STEMCELL Technologies, Vancouver, BC, CAN).
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bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... A single-step density gradient centrifugation was performed to isolate peripheral blood mononuclear cells (PBMCs) by slowly layering diluted blood (30 mL) over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) contained within either a 50 mL SepMate™ (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Cancer Biology 2023Quote: ... myeloid cells were gently thawed and washed in warm myeloid cell media (ImmunoCult™-SF Macrophage Differentiation Medium - using base media with only, M-CSF 50 ng/mL | STEMCELL Technologies, Cat. 10961), and plated in a 24-well low-attachment plate (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Genomics 2024Quote: ... Approximately 45 mg of flash-frozen skin or/fin was placed in a 6-well tissue culture plate (Stem Cell Technologies, cat. no. 38015) with 1 mL of TST buffer composed of 2 mL of 2X ST buffer ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: Neural progenitor cells (NPCs) were created from the hESCs described above using the STEMdiff SMADi Neural Induction kit from STEMCELL Technologies (Cat No. 08581) according to their guidelines for Matrigel-coated 6-well plates [31] ...
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bioRxiv - Developmental Biology 2023Quote: AGMs of E10.5 embryos were dissected in PBS with 7% fetal calf serum (FCS) and penicillin/streptomycin (100 U/mL) and cultured as explants for 2 days in medium consisting of Stemspan (Stem Cell Technologies, cat # 09600), 20% fetal calf serum ...
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bioRxiv - Cancer Biology 2023Quote: ... were treated with drugs at a final concentration of 10 µM and mixed with MethoCult™ H4100 Base medium (Stem cell technologies, Canada, 04100) enriched with 20% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... We seed HEBCs at the density of 4.2×104 cells/insert and add expansion medium (PneumaCult™-Ex Plus Medium, STEMCELL Technologies, Cat. No. 05040) to both the basal and apical chambers ...
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bioRxiv - Physiology 2023Quote: ... followed by thorough washing with cold PBS and incubated at room temperature in a gentle dissociation buffer (Stem cell technologies, Cat No. 100- 0485) for 20-30 minutes with gentle shaking ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were differentiated and matured using the StemCell Midbrain Neuron differentiation and maturation kit (#100-0038 and #100-0041; StemCell Technologies, New York, NY). Briefly ...
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bioRxiv - Genetics 2023Quote: ... and oligodendrocyte lineages following removal of EGF from NSE medium for one day and later by culture in NeuroCult™ Differentiation Medium (Stem Cell Technologies 05704) for an additional 9 days.
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats from healthy donors by density gradient centrifugation using Lymphoprep™ (STEMCELL Technologies Canada, Inc.). This procedure was approved by the Bioethics Committee of the Medical University of Warsaw ...
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bioRxiv - Microbiology 2024Quote: ... fibroblast) and Coriell Institute for Medical Research (Camden, NJ; ID#AG27602, Source; male, fibroblast) maintained in complete mTeSR+ medium (Stem Cell Technologies; Vancouver, Canada) containing 0.1% penicillin/streptomycin and propagated as small aggregates using accutase or ReLeSR (Stem Cell Technologies) ...
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bioRxiv - Immunology 2024Quote: CD4+CD25+ T cells isolated from the spleens of DR3 and DR3.IL17A-/- transgenic mice using the EasySep™ Mouse CD4+CD25+ regulatory T Cell Isolation Kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ T cells were isolated from splenocytes of DR3 and DR3.IL17A-/- transgenic mice using EasySep™ Mouse CD4+CD25+ regulatory T cell isolation kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... L0 and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753), 1% penicillin–streptomycin (Thermofisher ...
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bioRxiv - Developmental Biology 2021Quote: ... The media was then replaced with hTSC media containing 200 ng/ml recombinant B18R to prevent gamma-interferon response (Stem Cell Technologies; Cat. No. 78075). For reprogramming experiments lipofection was performed at the same time every day for 20 days ...
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bioRxiv - Developmental Biology 2022Quote: ... replated at a density of 1.58 × 105 cells/cm2 in Vitronectin XF-coated 24 well plate into cardiomyocyte media (RPMI, 2% B27 supplement plus insulin [Life Technologies Australia, Cat.#17504001], 10% FBS, and 10 µM Y-27362 ROCK inhibitor [Stem Cell Technologies, Cat.#72308]) and cultured overnight ...
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bioRxiv - Physiology 2021Quote: ... was opened and either placed into individual tubes containing cold DMEM (high glucose, 4.5 g/l) before immediately being processed for cell dissociation (Stemcell Technologies, Vancouver, BC, Canada, cat# 07174). Dissociated intestinal epithelial cells were stained with antibodies for CD31 ...
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bioRxiv - Microbiology 2020Quote: Whole blood samples from scrub typhus patients were collected and peripheral blood mononuclear cells (PBMC) were isolated using Lymphoprep™ density gradient medium and SepMate™ tubes (Stemcell Technologies, Vancouver, Canada). The isolated PBMC were aliquoted and stored at −80°C.
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 µM CHIR99021, a GSK3β inhibitor that activates Wnt signaling and limits apoptosis (Delepine et al., 2021; Qian et al., 2016) (Stem Cell Technologies, cat. no. 72054) and cultured 4 more days ...
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and 7AAD and purified by fluorescent activated cell sorting (FACS) at a density of 600 Lin- Sca1+cKit+7AAD- cells per 1.1 ml/well of MethoCult™ M3334 (STEMCELL Technologies, Vancouver, British Columbia, Canada). M3334 methocult was supplemented with cytokines to a final concentration of 5 ng/ml mIL-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... L0 and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753), 1% penicillin–streptomycin (Thermofisher ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... stem cells were cultured on Matrigel (FisherScientific, 354230) coated 6-well plates in mTeSR plus supplemented with 10% mTeSR plus Supplement (Stem Cell Technology, Cat 100-0276), and 1X Penicillin/Streptomycin or Primocin (FisherScientific NC9141851) ...
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bioRxiv - Immunology 2024Quote: ILCs from Alt and Alt plus BPC treated mice were enriched from two single-cell suspended lungs by Mouse Pan-ILC Enrichment Kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ILC enriched samples were preserved in PIPseqTM T100 3L Single Cell Capture and Lysis Kit and processed by sequencing service (Fluent BioSciences ...
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bioRxiv - Cell Biology 2022Quote: WIBR3 (NIHhESC-10-0079) hESCs and isogenic LIS1+/- 10 were grown and maintained on irradiated MEFs in optimal naive NHSM conditions (RSET-Stem Cell Technologies INC, supplementary Table 1) 90 ...
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bioRxiv - Developmental Biology 2021Quote: ... Medium was changed daily until cells were passaged at 80-90% confluency (medium supplemented with Y-27632 [cat. num. 72304; STEMCELL Technologies, Vancouver, Canada] for 24 hours) or induced into mesodermal differentiation at 30-40% confluency.