Labshake search
Citations for Stemcell Technologies :
4751 - 4800 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Genomics 2019Quote: ... and 8 weeks old adult mammary glands (#4 gland only) were dissected and pooled from multiple animals into dissection media (Epicult-B Basal medium (Stem Cell Technologies #05610) supplemented with 5% FBS ...
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bioRxiv - Genomics 2020Quote: ... Cells were passaged every four to six days as clumps using ReLeSR™ an enzyme-free reagent for dissociation (StemCell Technologies, Cat.: #05872) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: ... H9:SOX10 (Chambers et al., 2012), Mastershef7 (Gouti et al., 2014), and SFCi55-ZsGr (Lopez-Yrigoyen et al., 2018) were grown in mTESR (Stem Cell Technologies # 85850) on 1:100 dilution of Geltrex (ThermoFisher A1413202 ...
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bioRxiv - Immunology 2020Quote: Mouse bone marrow dendritic cells (DC) were generated as previously described.32 CD11c+ DC were purified by magnetic separation (EasySep Positive Selection; Stemcell Technologies, Cambridge, MA). Mature-DC were induced by adding LPS (200 ng/ml ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were prepared by mechanically disaggregating the domes and digesting the Matrigel for 1 hour using dispase at 37°C (Stem Cell Technologies, 7913). Cells were washed in PBS and resuspended in lysis buffer as previously described (Santiago-Gomez et al ...
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bioRxiv - Developmental Biology 2021Quote: NS cell cultures were established from E13.5 mouse ventral telencephalon as previously described (Conti et al. 2005) and maintained in mouse Neurocult basal media supplemented with proliferation supplement (Stem Cell Technologies, #05702,), 1% Penicillin-Streptomycin (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and then removed from the Parafilm and transferred to wells of a 24-well plate (one form per well) containing 1.5 mL of neural stem cell media (NeuroCultTM; 05700, STEMCELL Technologies, Vancouver, BC, Canada) supplemented with human EGF (20 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
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bioRxiv - Immunology 2020Quote: ... Two types of controls were used in all experiments except when noted: activated cells co-cultured in the presence of CD3/CD28/CD2 activating bead (STEMCELL Technologies, Vancouver, Canada) and non-activated cells co-cultured in the presence of complete media without beads ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells from each well were dissociated and resuspended in cloning media consisting of mTeSR-Plus supplemented with 1X CloneR2 (StemCell Technologies; 100-0691) and mixed gently with a P1000 pipette tip in order to generate a single-cell suspension ...
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bioRxiv - Bioengineering 2022Quote: ... after which the peripheral mononuclear cell fraction was isolated by carefully layering 25 mL diluted buffy coat onto 13 mL Lymphoprep (07851, StemCell technologies, Cologne, Germany) in separate 50 mL centrifugal tubes ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were digested the following day with Accutase and single-cell plated to 96-well plates in TeSR™-E8™ media with 0.25 μM Thiazovivin and CloneR™ supplement (StemCell Technologies) for clonal isolation and expansion as previously described [32] ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Neuroscience 2023Quote: ... serum was isolated through centrifugation and peripheral blood mononuclear cells (PBMCs) were isolated through Lymphoprep™ density gradient medium according to manufacturer instructions (Stemcell Technologies, Germany). The brains were then removed ...
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bioRxiv - Immunology 2024Quote: ... and mesenteric) from mice were sterilely collected in iron free media and homogenised through 40 μm filters using EasySep buffer (Stem Cell Technologies, 20144) or an in-house alternative (PBS + 2% FBS + 1mM EDTA (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was changed every second day and serial dilutions were performed in mTeSR+ medium containing Rock-inhibitor Y-27632 and CloneR (1:1000, Stem Cell Technologies #05889). Rock-inhibitor was removed after two days and the medium containing CloneR was changed every second day until colonies were ready to be picked ...
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bioRxiv - Developmental Biology 2024Quote: hiPSCs were thawed in presence of Rock-inhibitor Y-27632 (5µM, Stemgent-Ozyme #04-0012) and cultured under standard conditions at 37°C in mTeSR+ medium (Stem Cell Technologies #100-0276) on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... spectra were selected and seeded as single cell per well in a Geltrex coated 96-well plate containing E8 completed medium supplemented with Clone-R (STEMCELL Technologies, Vancouver, Canada). Plates were spun down at 400rcf for 1 minute to increase single cell attachment and were expanded at 37 °C in a humified atmosphere in the presence of 5% v/v CO2 until colonies were formed that could be used to generate a culturable cell line.
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 24-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276) and 1x Penn/Strep from a 100x stock (Mediatech ...
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bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood mononuclear cells (PMBCs) were isolated from patient blood using Lymphoprep™ density gradient centrifugation following manufacturer protocols (STEMCELL Tech; Vancouver, Canada) and confirmed to contain ≥ 90% CD19+/CD5+ cells before use ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ, Stem Cell Technologies). One well of seeded cells was left untreated as negative control (data not shown) ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... The obtained pellet is then washed and resuspended in cold RPMI media (Wisent Bioproducts. Purity and viability of the obtained neutrophils were verified through Methylene blue (Stem cell Technologies) and Trypan blue (Wisent ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI Differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium supplemented with 1X ALI Maintenance Supplement (StemCell Technologies), 0.48 ug/mL Hydrocortisone solution (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2019) were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs; DR4 ATCC) in PXGL medium47 or in RSeT medium (Stem Cell Technologies 05969), at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 7.5-9.0×104 cells were mixed with 0.5 mL culture medium and seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... the organoids were switched to maturation media in which BrainPhys Neuronal Media was replaced with BrainPhys Imaging Optimized Medium (Stem Cell Technologies # 5796). Calcium imaging was done using 4μM Fluo-8 AM (Abcam # ab142773) ...
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bioRxiv - Bioengineering 2023Quote: ... after which the peripheral mononuclear cell fraction was isolated by carefully layering 25 mL diluted buffy coat onto 13 mL Lymphoprep (07851, StemCell technologies, Cologne, Germany) in separate 50 mL centrifugal tubes ...
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bioRxiv - Developmental Biology 2023Quote: hPSC-derived cortical brain organoids were infected with lentiviruses encoding GCamp6m at day 50 of differentiation and cultured in BrainPhys™ Imaging Optimized Medium (Stem Cell Technologies) for a week before the imaging ...
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bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples were extracted from differentiated NPCs or undifferentiated NPCs treated for 2 hrs with STEMdiff™ Forebrain Neuron Differentiation medium (Stem Cell Technologies) for differentiated NPCs ...
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bioRxiv - Developmental Biology 2023Quote: PP cells generated using the STEMdiff™ Pancreatic Progenitor Kit and expanded under CINI conditions were dissociated into single cells using AccutaseTM (STEMCELL Technologies, 07920) at 37 °C and then resuspended in PEP medium (Table S4 ...
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bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ AML blast progenitor cells were purified by immuno-magnetic beads conjugated with anti-CD34 antibody following the manufacturer’s protocol (StemCell Technologies, Vancouver, British Columbia) prior to utilization in the cell viability assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Molecular Biology 2023Quote: ... NPCs were seeded in four Matrigel-coated 15-cm dishes per condition and allowed to reach 70-80% confluency in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies). Then ...
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bioRxiv - Cell Biology 2023Quote: CD34+ cells from the infusion product or purified from bone marrow mononuclear cells post-transplantation were plated in MethoCult H4435 Enriched media (STEMCELL Technologies, Vancouver, Canada) in 35-mm dishes at a concentration of 1000 cells/ml and incubated at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% penicillin–streptomycin and a 10 μM concentration of the ROCK inhibitor Y-27632 (Stem Cell Technologies, 72308). Further details for cell culture reagents and consumables can be found in the Resource Table and standard protocols can be found at www.allencell.org.
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected for single cell suspension and ILC enrichment (by Mouse Pan-ILC Enrichment Kit; STEMCELL Technologies, Vancouver, British Columbia, Canada). The dose of Alt was 30 µg/mouse ...
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bioRxiv - Immunology 2024Quote: ... the pooled blood was carefully layered over 5 mL of Ficoll-Paque PREMIUM 1.084 (Cytiva, Uppsala, Sweden) in 15 mL SepMate-50 tubes (STEM Cell Technology, Cambridge, MA). PBMCs were collected from the interphase layer after centrifugation at 1200xg for 30 min.
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bioRxiv - Genomics 2024Quote: ... hESCs were fed every day for mTeSR1 or every 2 days for mTeSR Plus and passaged every 5–6 days using ReLeSR (Stem Cell Technologies 05872).