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Citations for Stemcell Technologies :
701 - 750 of 9441 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were diluted 1:1 with phosphate-buffered saline (PBS) and MNC were enriched using SepMate-50 tubes (Stem Cell Technologies, 85460) and Pancoll separation solution (PAN Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred into 4 mL methylcellulose (Stem Cell Technologies, 4434) and vortexed vigorously ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Cancer Biology 2024Quote: Hypoxic incubator chamber (Cat# 27310) and a single flow meter (Cat# 27311) were purchased from Stemcell Technologies. Pre-mixed gas of 2% O2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The frequency of LSCs was calculated using Poisson statistics with the L-Calc Software for limiting dilution analysis (StemCell Technologies)
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bioRxiv - Cancer Biology 2024Quote: ... 0.1% of heparin (StemCell Technologies), and of EGF ...
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bioRxiv - Cancer Biology 2024Quote: ALDH activity was measured using an Aldefluor kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001 ...
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bioRxiv - Cancer Biology 2024Quote: ... was dispensed to a 50 mL SepMate™ (#85450, Stemcell Technologies,) tube ...
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bioRxiv - Cancer Biology 2024Quote: ... along with the “Big Easy” Magnet (Catalog #18001, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood ...
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bioRxiv - Cancer Biology 2024Quote: ... using mTeSR Plus medium (StemCell Technologies #100-0276). mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872) ...
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bioRxiv - Cancer Biology 2024Quote: ... which contained BrainPhys and SM1 (STEMCELL Technologies, Vancouver, #05792); ½ of the media was exchanged every 2–3 days ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... single cell suspension was thawed into ImmunoCult-XF media (Stemcell Technologies) and incubated at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... in ImmunoCult-XF media (Stemcell Technologies, 10981), and expanded for 2-3 weeks until reaching the appropriate amount of T cells (usually >10 x 106 cells) ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were dissociated with Accutase (STEMCELL Technologies, Vancouver, Canada) and seeded (0.5-3×105 cells/cm2 ...
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bioRxiv - Cancer Biology 2024Quote: ... coated tissue plates in NeuroCult NS-A proliferation kit media (Stem Cell Technologies Cat. #05751) supplemented with 1% penicillin/streptomycin solution (Sigma Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... heparin (2µg/ml, Stem Cell Technologies Cat. #07980), mEGF (20ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 25 μl accutase solution (Stemcell Technologies #07922) was added and left to incubate at room temperature for 10 min with gentle shaking ...
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bioRxiv - Cell Biology 2024Quote: ... and Heparin Solution (0.2%) (STEMCELL Technologies, 07980). CELLstart™ Humanized Substrate for Cell Culture (GIBCO ...
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bioRxiv - Cancer Biology 2024Quote: ... 3D organoids were harvested from the basement membrane matrix using Gentle Cell Dissociation Reagent (100-0485, StemCell Technologies), fixed with 4% paraformaldehyde ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in PBS (Hyclone, SH30256.01) supplementing with 0.005% DNase I (STEMCELL Technologies, 07900) at a concentration of 1×105 cells/μl ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were then treated with 2 ml pre-warmed Accutase (AT104, Stem Cell Technologies) and the vessel was incubated at 37ºC for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 0.5-1 x 107 cells/ml in “phase 1 expansion medium” consisting of Stemspan SFEM (Stem Cell Technologies) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Cell Biology 2024Quote: ... and 20% BIT (Stem Cell Technologies). The SCF was removed after day 3 of maturation and cells were cultured at 2X106 cells/ml from maturation day 4 to 7.
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Depending on the availability100 mm3-2000 mm3 cm tumor tissue piece was minced into smaller pieces and digested with 1x Collagenase & Hyaluronidase (# 7912, Stem Cell Technologies), or 1U/mL Dispase II (# D4693 ...
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: Plasma was isolated from extracted whole peripheral blood by adding heparin solution (STEMCELL Technologies, USA) followed by centrifugation at 1,300 g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells were removed from single cell suspension using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood mononuclear cells (PMBCs) were isolated from patient blood using Lymphoprep™ density gradient centrifugation following manufacturer protocols (STEMCELL Tech; Vancouver, Canada) and confirmed to contain ≥ 90% CD19+/CD5+ cells before use ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were enzymatically digested using Accutase (StemCell Technologies, Vancouver, BC, Canada Cat#07920) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... gliomaspheres were enzymatically digested using Accutase (StemCell Technologies, Vancouver, BC, Canada Cat#07920) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Cancer Biology 2024Quote: ... spleen-derived B-cells were selected using the EasySep™ Mouse Pan-B Cell Isolation Kit (STEMCELL Tech).
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bioRxiv - Cancer Biology 2024Quote: Human whole leukocytes from anonymous healthy donors were isolated from apheresis cones from ARUP using RBC sedimentation with HetaSep (STEMCELL Technologies, Vancouver, British Columbia). Freshly isolated whole leukocytes were used fresh for in vitro culture.
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bioRxiv - Cancer Biology 2024Quote: ... and Lymphoprep density gradient media (StemCell Technologies). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: The RosetteSep™ Human CD45 Depletion Cocktail (Catalog #15112, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood ...
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bioRxiv - Cancer Biology 2024Quote: ... cultured cells were transformed into a hypoxic chamber (Stemcell Technologies; Cat# 27310) filled with a pre-mixed gas of 2% O2 ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were isolated from cord blood (provided by Stanford Binns Program for Cord Blood Research) or sourced from Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technologies, Vancouver, Canada). Frozen Plerixafor- and/or G-CSF-mobilized peripheral blood of patients with sickle cell disease were provided by Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... PBMC were isolated from whole blood by density gradient centrifugation on Lymphoprep® (StemCell Technology) and cultured at 2x106 cells/mL in glutamine containing RPMI ...
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bioRxiv - Cancer Biology 2024Quote: ... referred to as stem cell medium (SCM, #05041, Stemcell Technologies, Vancouver, Canada) supplemented as described (15 ...
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bioRxiv - Bioengineering 2024Quote: ... ReLeSR (STEMCELL Technologies) was added to the flask and immediately aspirated away ...
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bioRxiv - Bioengineering 2024Quote: ... in mTeSR Plus stem cell medium (STEMCELL Technologies) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL of Human Recombinant IL-2 (STEMCELL TECHNOLOGIES, #78220) and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES ...
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bioRxiv - Bioengineering 2024Quote: ... coated tissue culture plates in mTeSR1 (Stemcell Technologies). Cardiomyocyte-directed differentiation was performed using a modified small molecule Wnt-modulating protocol using Chiron 99021 and IWP-4 as previously described.78,79 ...
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bioRxiv - Bioengineering 2024Quote: ... and suspended in 1 mL basal MesenCultTM (StemCell Technologies, Cambridge, MA) medium and added to the well in which a HMVEC monolayer was formed and incubated for 1 hour at 37°C ...