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Citations for Stemcell Technologies :
801 - 850 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and 1 µM CHIR99021, a GSK3β inhibitor that activates Wnt signaling and limits apoptosis (Delepine et al., 2021; Qian et al., 2016) (Stem Cell Technologies, cat. no. 72054) and cultured 4 more days ...
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bioRxiv - Developmental Biology 2024Quote: ... ALK7 inhibitor (Stem Cell Technologies, 72234) and 1 µM CHIR99021 ...
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bioRxiv - Developmental Biology 2024Quote: ... a sign of neuroepithelial induction were placed onto a single dimple on embedding sheet (Stem Cell Technologies, cat. no. 08579) and 15 µl of Matrigel ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... hESC colonies were dissociated with Gentle cell dissociation reagent (GCDR, Stem Cell Technologies, 07174) for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated with ACCUTASE(Stem Cell Technologies, 07920) and were passaged onto Matrigel-coated 12-well plates at 1.0×105 cells/well in mTESR-plus medium with 10 µM ROCK inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... All iPSC lines were cultured in mTESR-plus medium(Stem Cell Technologies, 100-0276) on matrigel-coated plates(Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Bioengineering 2024Quote: ... Either mTeSR1 medium (STEMCELL Technologies) or StemFit medium (Ajinomoto ...
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bioRxiv - Bioengineering 2024Quote: ... and 1X CD34+ Expansion supplement (StemCell Technologies).
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bioRxiv - Physiology 2024Quote: ... Cells where there treated with Gentle cell dissociation reagent (Stemcell Technologies, #100-0485) +1 μM Y-27632 for 10 minutes at RT ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Cancer Biology 2024Quote: ... PBMC were isolated from whole blood by density gradient centrifugation on Lymphoprep® (StemCell Technology) and cultured at 2x106 cells/mL in glutamine containing RPMI ...
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bioRxiv - Cancer Biology 2024Quote: ... referred to as stem cell medium (SCM, #05041, Stemcell Technologies, Vancouver, Canada) supplemented as described (15 ...
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bioRxiv - Bioengineering 2024Quote: ... and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES, #10971) for a duration of 3 days ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
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bioRxiv - Bioengineering 2024Quote: The primary T cells were obtained from peripheral venous blood of a healthy human donor using the EasySep™ Direct Human T cell Isolation Kit (STEMCELL TECHNOLOGIES, #19661) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... ReLeSR (STEMCELL Technologies) was added to the flask and immediately aspirated away ...
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bioRxiv - Bioengineering 2024Quote: ... in mTeSR Plus stem cell medium (STEMCELL Technologies) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL of Human Recombinant IL-2 (STEMCELL TECHNOLOGIES, #78220) and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES ...
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bioRxiv - Bioengineering 2024Quote: ... coated tissue culture plates in mTeSR1 (Stemcell Technologies). Cardiomyocyte-directed differentiation was performed using a modified small molecule Wnt-modulating protocol using Chiron 99021 and IWP-4 as previously described.78,79 ...
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bioRxiv - Bioengineering 2024Quote: ... and suspended in 1 mL basal MesenCultTM (StemCell Technologies, Cambridge, MA) medium and added to the well in which a HMVEC monolayer was formed and incubated for 1 hour at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... PBMCs were separated through Ficoll centrifugation using Lymphoprep (STEMCELL Technologies). PBMCs were washed and residual red cells lysed with ACK Lysis buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... EasySepTM Mouse CD8+ T cell isolation kit was purchased from Stemcell Technologies (19853).
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bioRxiv - Bioengineering 2024Quote: ... coated tissue culture plates in mTeSR1 (Stemcell Technologies). Cardiomyocyte directed differentiation was performed using a modified small molecule Wnt-modulating protocol using Chiron 99021 and IWP-4 as previously described.64,65 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... hESCs were cultured under feeder-free conditions in mTeSR Plus media (Stem Cell Technologies, 100-0276) on plates coated with Matrigel (Corning ...
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bioRxiv - Genetics 2024Quote: PBMCs were isolated using LymphoPrep medium (07851, STEMCELL Technologies) from patient’s blood ...
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bioRxiv - Bioengineering 2024Quote: ... in complete mTeSR plus medium (100-0276, Stem Cell Technologies). The iPSCs were passaged every 4 - 5 days when it reached 80 - 90% confluency using the StemPro® EZPassage™ disposable stem cell passaging tool (23181-010 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged ∼1:10 at 80% confluency using 0.5mM EDTA (STEMCELL Technologies) and small cell clusters (50–200 cells ...
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bioRxiv - Bioengineering 2024Quote: Two days post-electroporation 500 HSPCs were plated in each well of a SmartDish 6-well plate (STEMCELL Technologies, Vancouver, Canada, cat.: 27370) containing MethoCult H4434 Classic (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... containing MethoCult H4434 Classic (STEMCELL Technologies, cat.: 04444). After 14 days ...
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bioRxiv - Bioengineering 2024Quote: ... the wells were imaged using the STEMvision Hematopoietic Colony Counter (STEMCELL Technologies). Colonies were counted and scored with manual correction to determine the number of BFU-E ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA).
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bioRxiv - Bioengineering 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights™ EasySep™ Magnets (StemCell Technologies) according to the manufacturer protocol.
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bioRxiv - Bioengineering 2024Quote: ... and CD11b edited CD34+ HSPCs were counted and plated in duplicates in a SmartDish™ (StemCell Technologies) at a density of 200 cells/well in 1.1 mL of methylcellulose (Methocult H4435 Enriched ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Bioengineering 2024Quote: ... at a density of 200 cells/well in 1.1 mL of methylcellulose (Methocult H4435 Enriched, StemCell Technologies). This media contains SCF ...
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bioRxiv - Bioengineering 2024Quote: ... and 1X Myeloid Expansion Supplement II (StemCell Technologies). Cells were maintained for up to three weeks in the same plates and split every three to four days by discarding 160 μL of medium and adding 160 μL of fresh differentiation medium to each well.
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bioRxiv - Bioengineering 2024Quote: ... and classified using the STEMvision (StemCell Technologies) according to the ‘STEMvision™ Automated Colony-Forming Unit (CFU ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Bioengineering 2024Quote: hiPSCs were cultured in mTeSR Plus medium (STEMCELL Technologies, cat. no. 100-0276) on tissue culture plates coated with Matrigel (Corning ...
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bioRxiv - Cancer Biology 2024Quote: Peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained commercially (Stem Cell Technologies). For in vitro macrophage polarization experiments ...
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bioRxiv - Bioengineering 2024Quote: ... containing StemSpan SFEM II (StemCell Technologies) supplemented with penicillin/streptomycin (100 U/mL ...