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Citations for Stemcell Technologies :
651 - 700 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 3 µM CHIR9902 (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... and 1 x CC100 (StemCell Technologies) consisting of cytokines SCF ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 20% BITS 9500 (StemCell Technologies), 2 mM l-glutamine (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... per manufacturer’s instructions (STEMCELL Technologies, Vancouver). Purity (typically >85% ...
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Microbiology 2024Quote: ... first ultracentrifugation was followed by a density gradient ultracentrifugation using OptiPrep (Iodixanol, Stemcell technologies, cat# 07820). OMV were resuspended in 50% OptiPrep and OptiPrep gradient (10% ...
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bioRxiv - Immunology 2024Quote: Esophageal biopsies were dissociated (27) and CD45+ cells were removed using positive isolation beads (STEMCELL TECHNOLOGIES). The untouched epithelial-enriched cell fraction was washed with PBS ...
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bioRxiv - Microbiology 2024Quote: ... and IntestiCult™ Organoid Growth Medium (OGM, StemCell Technologies), that was replaced every other day ...
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bioRxiv - Microbiology 2024Quote: ... Differentiation was triggered by Wnt3a removal with IntestiCult™ Organoid Differentiation Medium (ODM, StemCell Technologies) for 5 days with medium changes every other day.
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bioRxiv - Microbiology 2024Quote: ... 5 µM CHIR99021 (StemCell technologies) and 1.25% V/V DMSO) ...
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bioRxiv - Microbiology 2024Quote: ... and 10 µM Y-27632 (StemCell Technologies) and inoculated with HEV or mock infected ...
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bioRxiv - Microbiology 2024Quote: ... medium was replaced by ODM supplemented with 5 µM CHIR99021 (StemCell technologies) and 10 µM Y-27632 ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... cord blood mononuclear cells were ordered from STEMCELL Technologies (STEMCELL Technologies, 70007.1 or 70007.2). NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... cord blood mononuclear cells were ordered from STEMCELL Technologies (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies, 17955) and activated by 100 Gy irradiated K562 Clone 9 feeder cells ...
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bioRxiv - Immunology 2024Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Natural Killer (NK) cells were isolated from Leukopaks (Stemcell Technologies) using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies) ...
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bioRxiv - Immunology 2024Quote: ... using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies). The isolated NK cells were incubated overnight at 37°C 5% CO2 in R10 (RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and subjected to a CD8+ T cell negative selection with the EasySepTM Mouse T cell isolation kit (STEMCELL Technologies cat#: 19853), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes or BM cells were enriched using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions from fluorescently labeled mice were mixed with methylcellulose-containing MethoCult M3534 (Stemcell Technologies) in various dilutions and incubated at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Freshly isolated HSPC populations (LT-HSC, ST-HSC, MPP, CLP, CMP, MDP) were resuspended in StemSpan medium (Stem Cell Technologies) supplemented with 50 ng/mL stem cell factor (SCF ...
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bioRxiv - Immunology 2024Quote: Total CD4+ T-cells were obtained by negative selection from the spleen of wild-type mice using EasySepTM magnetic separation per kit instructions (product number 19851, STEMCELL Technologies, Vancouver, Canada). Purified total CD4+ were enumerated and 5×105 total CD4+ T-cells were injected via tail vein to recipient mice ...
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bioRxiv - Immunology 2024Quote: ... were thawed on the day of the experiment and plated in each well supplemented with 50 ng/mL IFNγ (STEMCELL Technologies) for overnight activation ...
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bioRxiv - Immunology 2024Quote: ... and differentiated into monocyte-derived dendritic cells (moDCs) using the ImmunoCult Dendritic Cell Culture Kit (STEMCELL Technologies), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated using the EasySep Human CD4 Positive Selection Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Human CD14+ monocytes were isolated from leukopaks using the EasySep Human CD14+ Positive Selection Kit (STEMCELL Technologies) and differentiated into monocyte-derived dendritic cells (moDCs ...
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bioRxiv - Immunology 2024Quote: ... frozen human PBMCs (STEMCELL Technologies, Vancouver, Canada) were thawed and seeded in a 75 cm2 flask at a concentration of 5 × 106 cells/mL in RPMI 1640 media (Corning Inc. ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of RALDH2 in BMDCs and MLN cells was assesses using the ALDEFLUOR Kit (#01700, STEMCELL Technologies, Vancouver, BC, Canada). DCs were pre-gated as CD11c+/MHC IIhigh cells and RALDH2 activity was detected by a flow cytometric analysis.
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2024Quote: ... The manufacturer’s instructions were followed to isolate PBMCs using LymproprepTM (Stemcell Technologies; Ref. 07811) and either a Sepmate™-15 or −50 (Stemcell ...
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bioRxiv - Immunology 2024Quote: ... The digested sample was filtered through a 70 µm strainer (Stemcell Technologies; Cat. 27260). The strainer was rinsed 5 times with 1 mL ice-cold DPBS+/+ ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... CD11c+ DCs were positively magnetically isolated (STEMCELL Technologies). DCs were plated at a 1:2 ratio with Tregs and left to co-incubate for 2 days ...
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bioRxiv - Immunology 2024Quote: ... using magnetic-based CD14- or CD15-positive selection kits (STEMCELL TECHNOLOGIES, CD15+ positive selection REF#18651 ...
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bioRxiv - Immunology 2024Quote: ... and CD4+ T cells were enriched via magnetic negative selection (STEMCELL Technologies). Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605 ...
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bioRxiv - Immunology 2024Quote: ... The remaining cellular pellet volume was measured and the red blood cells lysed with ammonium chloride (STEMCELL Technologies). Cells were then stained to identify viability dyenegative CD45+ (BUV395 ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads were magnetically removed and Thy1.1+ cells were positively selected (STEMCELL Technologies). Tregs rested overnight until day 8 ...
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bioRxiv - Immunology 2024Quote: ... sex-matched NOD/ShiLtJ or NOD-HLA-A2 mice and enzymatically digested (STEMCELL Technologies). CD11c+ DCs were positively magnetically isolated (STEMCELL Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were subsequently isolated using the EasySep™ Human Monocyte Isolation Kit (StemCell Technologies) in accordance with the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were generated and enriched for CD4+ T cells by negative selection using EasySep magnetic bead kit (STEMCELL Technologies) and stained with anti-mouse CD4 (RM4-5 ...
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bioRxiv - Immunology 2024Quote: ... FITC-CD14 (MoP9, STEMCELL Technologies #60124FI).
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bioRxiv - Immunology 2024Quote: ... https://vi-talant.org/Home.aspx) by using the negative selection “RosetteSep Human NK Cell Enrichment Cocktail” kit (#15065; STEMCELL Technologies). Primary NK cells were cultured in CellGenix® GMP stem-cell growth media (SCGM ...
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bioRxiv - Immunology 2024Quote: ... gated for cKit+Sca1+CD11b−IL7Rα− cells and then single cell index sorted into 96-well round-bottom plates containing StemSpan™ SFEM II (Stemcell Technologies) supplemented with 50 ng/mL stem cell factor (in-house ...
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bioRxiv - Immunology 2024Quote: Human iNKT cells were enriched by magnetic bead-based positive selection (Stem Cell Technologies EasySep™ Release Human PE Positive Selection Kit ...