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Citations for Stemcell Technologies :
751 - 800 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and an EasySep Magnet (STEMCELL Technologies, Vancouver, Canada) as per the manufacturer’s protocol ...
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Cyborg islets: implanted flexible electronics reveal principles of human islet electrical maturationbioRxiv - Bioengineering 2024Quote: ... and then incubated in 6.5 mL DPBS and 8 mL Accutase (StemCell Technologies) for 7 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1% heparin (StemCell Technologies), and EGF ...
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bioRxiv - Cancer Biology 2024Quote: ... The medium for BT-245 consisted of Neuro-cult with proliferation supplement (StemCell Technologies), 1% of pen-strep antibiotic (GIbco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated from healthy donor’s blood using density gradient centrifugation with Lymphoprep™ (STEMCELL Technologies) as the separation medium ...
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bioRxiv - Cell Biology 2024Quote: ... Medium was replaced on the basolateral side with PneumaCult medium (STEMCELL Technologies) which was prepared as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... using the EasySep™ Naïve CD4+ T cell isolation kit (STEMCELL Technologies) according to isolation procedures reported previously.10 Cells were cultured in serum-free AIM V media (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada), CD3Ab OKT3 (Sino Biological ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Bioengineering 2024Quote: ... We transition the cultures to ALI by removing the apical medium and replacing PneumaCult™- Ex Plus Medium with PneumaCult™-ALI Medium (STEMCELL Technologies, Cat. No. 05001). The medium is changed every other day ...
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bioRxiv - Bioengineering 2024Quote: ... at the density of 4.2×104 cells/well and add PneumaCult™-Ex Plus Medium (STEMCELL Technologies, Cat. No. 05040) to both the basal and apical chambers ...
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bioRxiv - Bioengineering 2024Quote: ... . These samples were cultured for seven days in StemSpan SFEM II (STEMCELL Technologies, 09655) supplemented with a cytokine cocktail that supports hematopoietic stem cell growth and differentiation ...
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bioRxiv - Bioengineering 2024Quote: ... and 6 × 103 human bone marrow CD34+ cells (STEMCELL Technologies, 70002.3) into a collagen-based hydrogel according to the protocol described previously (49 ...
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bioRxiv - Bioengineering 2024Quote: ... coated dishes using mTeSRTM Plus medium (Stemcell Technologies; 100-0276). When healthy colonies of cells were formed ...
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bioRxiv - Bioengineering 2024Quote: ... we culture HBECs using PneumaCult™-Ex Plus Medium (STEMCELL Technologies, Cat. No. 05040). We passage HBECs using AccutaseTM Cell Detachment Solution (Innovative Cell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells dissociated with Accutase were either cryopreserved using CryoStor® CS10 (Stemcell technologies; 07959) for later use or mixed with Matrigel (Corning ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were suspended in CryoStor 10 (StemCell Technologies, Seattle, WA), aliquoted ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Bioengineering 2024Quote: ... 600000 single stem cells were seeded per well (500 cells/microwell) of AggreWell™400 (STEMCELL Technologies, Vancouver, Canada) plates in aggregation media with 50 μM Y27632 (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mg/mL dispase II (STEMCELL Technologies, Vancouver, Canada) for 4 hours under gentle agitation at 37° C ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were sourced from OriBiotech (Shanghai, China) and cultured in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada). Mouse PBMCs were purified from mouse whole blood by Mouse PBMC Isolation Kit (Solarbio ...
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bioRxiv - Bioengineering 2024Quote: ... human T cells were obtained from fresh Leukopaks purchased from STEMCELL Technologies (Seattle, WA). T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... plates in aggregation media with 50 μM Y27632 (STEMCELL Technologies). Mesoderm induction and sprouting was induced directly in the AggreWell™400 plates by carefully changing the media with a p1000 pipette making sure to not disturb the cell aggregates in the microwells ...
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bioRxiv - Cancer Biology 2024Quote: ... Passaging was with ReLeSR passaging reagent (Stem Cell Technologies). Due to their highly adhesive characteristics ...
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bioRxiv - Cancer Biology 2024Quote: Both WT and MT iPS cells were maintained in mTeSRTM1 medium with 1x Supplement media (Stem Cell Technologies, Catalog #85850) and supplemented with 10 μM Y27632 (Stemgent ...
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bioRxiv - Cancer Biology 2024Quote: ... mesoderm or ectoderm using the STEMdiff Trilineage Differentiation Kit Protocol (Stem Cell Technologies). After differentiation ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were passaged using Accutase (StemCell Technologies, 07920) and plated at a density of 100k cells/well ...
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bioRxiv - Bioengineering 2024Quote: ... medium from the upper chamber was removed and replaced with ATDC5 medium containing 0 or 0.1 ng/ml IL-1α (STEMCELL Technologies). Following two additional days of incubation ...
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bioRxiv - Bioengineering 2024Quote: ... ROCK inhibitor (STEMCELL Technologies, 72304) and blebbistatin (Tocris ...
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bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... coated dishes under mTeSRTM Plus medium (Stemcell Technologies; 100-0276) and passaged using the StemProTM EZPassageTM passaging tool (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... or ReLeSRTM human PSC selection and passaging reagent (Stemcell Technologies; 05872). The differentiation of iPSCs into foregut cells was performed using previously published protocols13,21 ...
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bioRxiv - Bioengineering 2024Quote: ... or cryopreserved using CryoStor® CS10 (Stemcell technologies; 07959) for later use ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were diluted 1:1 with phosphate-buffered saline (PBS) and MNC were enriched using SepMate-50 tubes (Stem Cell Technologies, 85460) and Pancoll separation solution (PAN Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred into 4 mL methylcellulose (Stem Cell Technologies, 4434) and vortexed vigorously ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Cancer Biology 2024Quote: Hypoxic incubator chamber (Cat# 27310) and a single flow meter (Cat# 27311) were purchased from Stemcell Technologies. Pre-mixed gas of 2% O2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The frequency of LSCs was calculated using Poisson statistics with the L-Calc Software for limiting dilution analysis (StemCell Technologies)
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Cancer Biology 2024Quote: ... and heparin (Stem Cell Technologies, 07980). 500 cells per well from LD 12:12 and CRD group tumors were added and incubated with standard cell culture conditions for 5 days in the CO2 incubator before being observed under the microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... human recombinant bFGF (hrbFGF) (Stem Cell Technologies, 78003), and heparin (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1% of heparin (StemCell Technologies), and of EGF ...
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bioRxiv - Cancer Biology 2024Quote: ALDH activity was measured using an Aldefluor kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Freshly isolated human HSPCs were plated at 200,000 cells/ml and cultured in IMDM medium supplemented with 25% BIT9500 (StemCell Technologies), 1% penicillin/streptomycin (P/S) ...
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bioRxiv - Cancer Biology 2024Quote: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001 ...