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Citations for Stemcell Technologies :
851 - 900 of 9441 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (StemCell Technologies).
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bioRxiv - Neuroscience 2024Quote: Microglia or macrophages were detached using Accutase (StemCell Technologies) and washed twice in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were detached using Accutase (StemCell Technologies) and dissociated into a single cell suspension ...
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bioRxiv - Neuroscience 2024Quote: ... using Gentle Cell Dissociation Reagent (StemCell Technologies). hiPSCs were cultured on the feeder layer in hiPSC base medium ...
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bioRxiv - Neuroscience 2024Quote: ... After nucleofection cells were plated onto a 10 cm dish coated with Synthemax (5 μg/cm2) with TeSR-E8 supplemented with CloneR (StemCell Technologies). Cells were cultured to 80% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... Macroscopic lung nodules were dissected separately and digested in Collagenase Type IV (Stem Cell technologies; 07909) at 37°C for 1.5 h with rocking ...
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bioRxiv - Neuroscience 2024Quote: ... live cells were counted with Trypan Blue (Stem Cell Technologies) in a Burker counting chamber ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs in NPC medium were dissociated with Accutase (Stem Cell Technologies), live cells were counted with Trypan Blue (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... BrainPhys Neuronal Media (Stem Cell Technologies), 1% N2 supplement (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% minimum essential medium/non-essential amino acid (Stem Cell Technologies), 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304). Plates were coated with Gibco Geltrex Growth Factor Reduced Basement Membrane (Thermo catalog # A1413202) ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were released with Accutase (STEMCELL Technologies cat #07920) and plated at a high density of (250kcells/cm2 in 6-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at a high density of (250kcells/cm2 in 6-well plates) as a single-cell suspension in StemDiff Neural Induction Media with Dual SMAD Inhibition (STEMCELL Technologies cat #08581), supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304) ...
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bioRxiv - Neuroscience 2024Quote: ... or ReLeSR (STEMCELL technologies, 100-0484). ESCs passaged with Accutase were maintained for 24 hours with mTeSR1 supplemented with 10 μM of the ROCK kinase inhibitor ...
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bioRxiv - Immunology 2024Quote: ... splenocytes in a single cell suspension were negatively selected for CD4+ or CD8+ T-lymphocytes using the EasySep Mouse CD4+ T-cell Isolation Kit or CD8+ T-cell Isolation Kit (StemCell Technologies #19852, #19853, Vancouver, BC, USA), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... The T cells were cultured for 48 hours in ImmunoCult medium (STEMCELL Technologies Canada Inc., Vancouver, BC) for the control condition ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... and Valproic Acid (VPA, 10 μM final concentration; #72292, Stem Cell Technologies, Canada) to enrich organoids for stem cells.82 After approximately 5 – 7 days ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... organoids were removed from Matrigel and passaged with a split ratio of 1:4 using the protocol published by Stem Cell Technologies for use with their mouse IntestiCult system ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Pellets were resuspended in pre-warmed IntestiCult Organoid Growth Medium (150 μL; #06005, Stem Cell Technologies, Canada). Matrigel (150 μL ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... intestinal segments were resuspended in room temperature Gentle Cell Dissociation Reagent (25 mL; #07174, Stem Cell Technologies, Canada) and incubated on a rocking platform ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Intestinal organoid culture was established from 20 to 25-week-old female C57BL/6 mice by harvesting intestinal epithelial crypts using a protocol published by Stem Cell Technologies for use with their mouse IntestiCult system ...
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bioRxiv - Cell Biology 2024Quote: ... treated monolayers were washed in PBS then incubated in 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The organoids were dissociated using Accumax (Stem Cell Technologies, 07921) at 37°C/5% CO2 for 35 minutes and pipetted twenty times with a P-1000 wide-bore pipette tip every 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... We added an enzyme cocktail (Accumax, STEMCELL Technologies Inc., Cambridge, MA) to the minced tissue at 50% of the volume of minced tissue (e.g. ...
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bioRxiv - Cell Biology 2024Quote: ... All iPSC cultures were passaged with Dispase (1 mg/ml; Stem Cell Technologies). Cultures were routinely analyzed by PCR for mycoplasma contamination/DNA (Universal Mycoplasma Detection Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1.0 mg/mL collagenase V (STEMCELL Technologies, 100-0681), followed by further mechanical disruption by pipetting ...
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bioRxiv - Cell Biology 2024Quote: ... SB4315422 (10 μM, #72232; STEMCELL Technologies), and DMH-1 (10 μM ...
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bioRxiv - Cell Biology 2024Quote: Feeder-free human iPSCs were maintained in mTeSR1 (Stem Cell Technologies) on hESC-qualified Matrigel (BD Biosciences ...
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bioRxiv - Genomics 2024Quote: ... for at least one passage before differentiation into CNCCs or mTeSR Plus (Stem Cell Technologies 100–0276) for gene editing ...
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bioRxiv - Genomics 2024Quote: ... hESCs were fed every day for mTeSR1 or every 2 days for mTeSR Plus and passaged every 5–6 days using ReLeSR (Stem Cell Technologies 05872).
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bioRxiv - Genomics 2024Quote: ... cells were maintained in mTeSR 1 (STEMCELL Technologies, 85850) and the medium was changed daily ...
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bioRxiv - Immunology 2024Quote: ... Peripheral mononuclear blood cells (PBMCs) were collected using SepMate-50 PBMC Isolation Tubes (STEMCELL Technologies) and frozen slowly at -80 C before transfer to liquid nitrogen for long-term storage ...
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bioRxiv - Genomics 2024Quote: ... the tissue pieces were incubated in Gentle Cell Dissociation Reagent (STEMCELL Technologies, Germany) while gently rocking for 30 minutes at room temperature ...
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bioRxiv - Genetics 2024Quote: ICF2- and control-iPSCs were differentiated into hematopoietic progenitors using the STEMdiff Hematopoietic Kit (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2024Quote: ... taken up in Cryostor® CS10 freezing medium (07930; Stemcell Technologies) at 5 x 106 cells/mL and stored at -150°C.
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bioRxiv - Immunology 2024Quote: ... In short, PBMCs were pelleted (300g, 5 min) and resuspended in EasySep™ Buffer (20144; Stemcell Technologies) at a concentration of 1 x 108 cells/mL and incubated with 100 µL selection antibody cocktail per mL of sample for 10 min at RT ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated from PBMCs by CD14 positive selection using the EasySep™ Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Total CD4 T cells were isolated using EasySep mouse CD4+ T cell isolation kit (Stemcell technologies) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... and the dead cells were removed using EasySep™ Dead Cell Removal (Annexin V) Kit (Stemcell technologies) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were isolated using a negative selection kit (STEMCELL Technologies). The purified naïve CD4+ T cells ...
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bioRxiv - Immunology 2024Quote: ... The samples were enriched for CD4 T cells using the EasySep™ mouse CD4+ T Cell isolation kit (Stemcell technologies) and the dead cells were removed using EasySep™ Dead Cell Removal (Annexin V ...
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bioRxiv - Immunology 2024Quote: Bone-marrow monocytes were purified from bone marrow cellular suspensions using an EasySepTM Mouse Monocyte Isolation Kit (STEMCELL Technologies), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1X StemSpan™ Myeloid Expansion Supplement (Stemcell Technologies, Catalog # 02693) containing SCF ...
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bioRxiv - Genomics 2024Quote: ... dissociated with accutase (STEMCELL Technologies, 07920) and plated a at 1:6 ratio in matrigel (Corning ...
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bioRxiv - Immunology 2024Quote: ... 10 µM Y27632 (Stemcell Technologies, 72304), 100 ng/mL IGF-1 (Biolegend ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from the blood of healthy volunteers using Lymphoprep medium (STEMCELL Technologies). Miltenyi human classical monocyte cell isolation kit (130-117-337 ...
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bioRxiv - Immunology 2024Quote: ... incubated for 5 min with 125 µL/mL RapidSphere beads (StemCell Technologies), then volume topped up to 2.5 mL and placed in EasySep magnet for 2.5 min ...
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bioRxiv - Immunology 2024Quote: ... resuspended in PBS containing 0.04% bovine serum albumin (BSA, STEMCELL Technologies) and loaded on a 10x Chromium (10x Genomics ...
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bioRxiv - Genomics 2024Quote: ... we seeded thawed iPSC at passage 12 (P12) or above in Matrigel-coated 6-well plates in mTESR1 media (STEMCELL Technologies (SCT), Vancouver ...
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bioRxiv - Genomics 2024Quote: ... and 0.48 µg/mL Hydrocortisone (Stem Cell Technologies, 74144) on a stir plate at 37□C and 180 rpm ...