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Citations for Stemcell Technologies :
501 - 550 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Positive selection of CD34+ cells was then performed on the low-density cells using the EasySep™ kit from STEMCELL Technologies) to obtain final CD34+ purities ...
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bioRxiv - Immunology 2024Quote: ... cells were rapidly thawed at 37 °C in a water bath and added dropwise into IMDM (STEMCELL Technologies) containing 10% FBS and 10 μg/mL DNase (MilliporeSigma) ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... per manufacturer’s instructions (STEMCELL Technologies, Vancouver). Purity (typically >85% ...
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bioRxiv - Genomics 2024Quote: ... cells were grown in Neurocult NS-A Proliferation Media (StemCell) supplemented with 0.0002% heparin (StemCell Technologies), EGF (20 ng/ml) ...
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bioRxiv - Immunology 2024Quote: ... and subjected to a CD8+ T cell negative selection with the EasySepTM Mouse T cell isolation kit (STEMCELL Technologies cat#: 19853), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... CD3+ T cells were isolated by magnetic bead separation (StemCell Technology) from PBMCs of three leukopaks (Cambridge Biosciences ...
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bioRxiv - Genomics 2024Quote: ... Approximately 45 mg of flash-frozen skin or/fin was placed in a 6-well tissue culture plate (Stem Cell Technologies, cat. no. 38015) with 1 mL of TST buffer composed of 2 mL of 2X ST buffer ...
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bioRxiv - Genomics 2024Quote: ... using bead separation (Stemcell Technologies) and cryopreserved ...
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bioRxiv - Genomics 2024Quote: ... seeded in 400-microwell Aggrewells (Stemcell Technologies, catalog no. 34450) and centrifuged at 100 x g for 3 min to evenly distribute the cells in microwells ...
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bioRxiv - Genomics 2024Quote: ... mouse NIH-3T3 cells were washed once with PBS and dissociated with Accutase (Stem Cell Technologies, catalog no. 07920). Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 10 vol% conditioned medium containing Rspondin1 (produced from HA-R-Spondin1-Fc HEK293T cell line ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 3 µM CHIR9902 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). 24 h after transfection ...
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bioRxiv - Genomics 2024Quote: ... 24 x 106 SNRPN-2A-GFP VP64dCas9VP64 (maternal) or dCas9KRAB (paternal) iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850 ...
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bioRxiv - Genomics 2024Quote: ... 1.7 x 106 matSNRPN-2A-GFP Tet1cdCas9 or VP64dCas9VP64 iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850 ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼430-fold coverage of the gRNA library ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼580-fold coverage of the gRNA sub-library ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Genomics 2024Quote: ... 250 µL of ImmunoCult Human CD3/CD28 T Cell Activator Mix (StemCell Technology) was added to the cells and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: CD3+ T cells were isolated from healthy donor leukopaks (Stemcell Technologies) using bead separation (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Genomics 2024Quote: ... and 1 x CC100 (StemCell Technologies) consisting of cytokines SCF ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 20% BITS 9500 (StemCell Technologies), 2 mM l-glutamine (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... ROCK-inhibitor (Ri) Y27672 (10 µM; Stem Cell Technologies) was always employed in single cell suspension conditions to improve viability ...
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bioRxiv - Developmental Biology 2024Quote: hESCs were routinely cultured in mTeSR1 (STEMCELL Technologies) medium ...
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bioRxiv - Developmental Biology 2024Quote: ... 6X105 cells were plated in 24-well dishes AggreWell400 pretreated with AggreWell Rising solution (STEMCELL Technologies) in EBM2 medium for 16 h to allow the formation of spheroids containing ∼ 500 cells ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Developmental Biology 2024Quote: ... in IntestiCult Organoid Growth Medium (Mouse) (Stem Cell Technologies, cat.# 06005) supplemented with 1% (vol/vol ...
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bioRxiv - Developmental Biology 2024Quote: ... 25 mL Gentle Cell Dissociation Reagent (Stem Cell Technologies, cat.# 100-0485) were added ...
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bioRxiv - Developmental Biology 2024Quote: ... resuspended in 100 μL phosphate buffered saline (PBS) without Ca2+ and Mg2+ (D-PBS; Stem Cell Technologies, cat.# 37350) and 20 μL aliquots were prepared and stored at -80°C.
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bioRxiv - Cell Biology 2024Quote: ... the iPSCs were maintained in mTesR1 (StemCell Technologies, #85850) on matrigel-coated tissue culture ware ...
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bioRxiv - Cell Biology 2024Quote: KOLF2 human iPSCs were sourced from Public Health England Culture Collections (catalogue number 77650059 and 77650100, respectively) and maintained in Vitronectin XF (StemCell Technologies) coated plates with E8 medium (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and seeded into AggreWell 800 plates (StemCell Technologies) with 4×106 cells/well and centrifuged at 100 g for 3 min ...
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bioRxiv - Cell Biology 2024Quote: FL and BM colony forming assays in methylcellulose (MethoCult M3434, Stemcell Technologies, Vancouver, Canada) were performed according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... and passaged using ReLeSR (StemCell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... and N2 supplement-A (StemCell Technologies, #07152)] was subsequently added in at increasing concentrations (25-100% in NM ...
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bioRxiv - Cell Biology 2024Quote: ... 42] iPSCs were maintained in mTESR (StemCell Technologies) on Matrigel (Corning Matrigel hESC-qualified Matrix ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Cell Biology 2024Quote: ... each containing 100 uL of StemSpan SFEM II medium (STEMCELL Technologies), supplemented with 1% penicillin-streptomycin (GIBCO) ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of RALDH2 in BMDCs and MLN cells was assesses using the ALDEFLUOR Kit (#01700, STEMCELL Technologies, Vancouver, BC, Canada). DCs were pre-gated as CD11c+/MHC IIhigh cells and RALDH2 activity was detected by a flow cytometric analysis.
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bioRxiv - Immunology 2024Quote: Human iNKT cells were enriched by magnetic bead-based positive selection (Stem Cell Technologies EasySep™ Release Human PE Positive Selection Kit ...