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Citations for Stemcell Technologies :
551 - 600 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Freshly isolated HSPC populations (LT-HSC, ST-HSC, MPP, CLP, CMP, MDP) were resuspended in StemSpan medium (Stem Cell Technologies) supplemented with 50 ng/mL stem cell factor (SCF ...
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bioRxiv - Immunology 2024Quote: Total CD4+ T-cells were obtained by negative selection from the spleen of wild-type mice using EasySepTM magnetic separation per kit instructions (product number 19851, STEMCELL Technologies, Vancouver, Canada). Purified total CD4+ were enumerated and 5×105 total CD4+ T-cells were injected via tail vein to recipient mice ...
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bioRxiv - Immunology 2024Quote: ... were thawed on the day of the experiment and plated in each well supplemented with 50 ng/mL IFNγ (STEMCELL Technologies) for overnight activation ...
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bioRxiv - Immunology 2024Quote: ... and differentiated into monocyte-derived dendritic cells (moDCs) using the ImmunoCult Dendritic Cell Culture Kit (STEMCELL Technologies), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Genomics 2024Quote: ... A specialized medium was created by mixing 10% CloneR™ (StemCell Technologies #05888) and 10 ng/l Pifithrin-α in mTeSR™ medium ...
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bioRxiv - Genomics 2024Quote: ... 1 µM doxycycline and 10% CloneR™ (StemCell Technologies #05888) in mTeSR medium ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... CD11c+ DCs were positively magnetically isolated (STEMCELL Technologies). DCs were plated at a 1:2 ratio with Tregs and left to co-incubate for 2 days ...
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bioRxiv - Immunology 2024Quote: ... using magnetic-based CD14- or CD15-positive selection kits (STEMCELL TECHNOLOGIES, CD15+ positive selection REF#18651 ...
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bioRxiv - Immunology 2024Quote: ... and CD4+ T cells were enriched via magnetic negative selection (STEMCELL Technologies). Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605 ...
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bioRxiv - Immunology 2024Quote: ... The remaining cellular pellet volume was measured and the red blood cells lysed with ammonium chloride (STEMCELL Technologies). Cells were then stained to identify viability dyenegative CD45+ (BUV395 ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads were magnetically removed and Thy1.1+ cells were positively selected (STEMCELL Technologies). Tregs rested overnight until day 8 ...
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bioRxiv - Immunology 2024Quote: ... sex-matched NOD/ShiLtJ or NOD-HLA-A2 mice and enzymatically digested (STEMCELL Technologies). CD11c+ DCs were positively magnetically isolated (STEMCELL Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were subsequently isolated using the EasySep™ Human Monocyte Isolation Kit (StemCell Technologies) in accordance with the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were generated and enriched for CD4+ T cells by negative selection using EasySep magnetic bead kit (STEMCELL Technologies) and stained with anti-mouse CD4 (RM4-5 ...
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bioRxiv - Immunology 2024Quote: ... FITC-CD14 (MoP9, STEMCELL Technologies #60124FI).
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bioRxiv - Immunology 2024Quote: ... + 10 µM Y-27632 ROCK inhibitor (Stemcell Technologies) (only for the first two days ...
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bioRxiv - Immunology 2024Quote: ... Freshly harvested organ tissue from male and female bats was washed in PBS and cut into 3-5 mm small pieces before placing up to four individual pieces into a 1 ml cryostorage tube containing 0.5ml-1ml of Cryostore10 freezing medium (Stemcell Technologies). Vials were immediately transferred to a cryostorage container (Mr ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human FGF2 (Stemcell Technologies) + 500 nM A-83-01 + 1:500 Primocin + 10 µM Y-27632 (only for the first two days) ...
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bioRxiv - Immunology 2024Quote: ... + 10 µM SB-431542 (Stemcell Technologies) + 1 µM BIRB796 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 3 µM CHIR9902 (Stemcell Technologies) + 10 µM SB-431542 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 1 µM BIRB796 (Stemcell Technologies) were added to the culture medium ...
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bioRxiv - Immunology 2024Quote: ... after which they were air-lifted by removing medium in the apical chamber and replacing the basal chamber medium with complete PneumaCult air-liquid interface medium (Stemcell Technologies) + 1:500 Plasmocin ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... treated cell culture dishes in PneumaCult-Ex plus expansion medium (Stemcell Technologies). Nasal epithelial cells were expanded for up to two additional passages in PneumaCult-Ex plus expansion medium and cryopreserved in liquid nitrogen in Cryostore10 freezing medium ...
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bioRxiv - Immunology 2024Quote: ... dead cells were removed by AnnexinV-based magnetic bead selection following the manufactureŕs instructions (Stemcell Technologies). In short ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated using the EasySep Human CD4 Positive Selection Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Human CD14+ monocytes were isolated from leukopaks using the EasySep Human CD14+ Positive Selection Kit (STEMCELL Technologies) and differentiated into monocyte-derived dendritic cells (moDCs ...
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bioRxiv - Immunology 2024Quote: ... https://vi-talant.org/Home.aspx) by using the negative selection “RosetteSep Human NK Cell Enrichment Cocktail” kit (#15065; STEMCELL Technologies). Primary NK cells were cultured in CellGenix® GMP stem-cell growth media (SCGM ...
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bioRxiv - Immunology 2024Quote: ... gated for cKit+Sca1+CD11b−IL7Rα− cells and then single cell index sorted into 96-well round-bottom plates containing StemSpan™ SFEM II (Stemcell Technologies) supplemented with 50 ng/mL stem cell factor (in-house ...
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bioRxiv - Immunology 2024Quote: ... frozen human PBMCs (STEMCELL Technologies, Vancouver, Canada) were thawed and seeded in a 75 cm2 flask at a concentration of 5 × 106 cells/mL in RPMI 1640 media (Corning Inc. ...
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bioRxiv - Immunology 2024Quote: ... Organoids were dissociated by accumax (STEMCELL Technologies, 07921). Briefly ...
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bioRxiv - Immunology 2024Quote: Female CatchupIVM-red and FVB/N mice aged 8-15 weeks were treated with 5 µg mouse recombinant G-CSF (STEMCELL Technologies) by intraperitoneal injection (i.p. ...
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bioRxiv - Immunology 2024Quote: Human T cells were isolated from healthy donors who provided informed consent using RosetteSep Human T-Cell Enrichment Cocktail (STEMCELL Technologies, Canada) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2024Quote: ... The manufacturer’s instructions were followed to isolate PBMCs using LymproprepTM (Stemcell Technologies; Ref. 07811) and either a Sepmate™-15 or −50 (Stemcell ...
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bioRxiv - Immunology 2024Quote: ... The digested sample was filtered through a 70 µm strainer (Stemcell Technologies; Cat. 27260). The strainer was rinsed 5 times with 1 mL ice-cold DPBS+/+ ...
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bioRxiv - Immunology 2024Quote: ... the cells were detached by Accutase (Stemcell Technologies) treatment at 37°C for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... were expanded in StemSpan SFEM II (StemCell Technologies, 09655), supplemented with 100 ng/mL human recombinant SCF ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Microbiology 2024Quote: ... were maintained in mTeSR1 (StemCell Technologies, Saint Egreve, France) on a matrigel coating (Corning ...
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bioRxiv - Microbiology 2024Quote: ... and dissociated with dispase (StemCell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Differentiation was triggered by Wnt3a removal with IntestiCult™ Organoid Differentiation Medium (ODM, StemCell Technologies) for 5 days with medium changes every other day.
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bioRxiv - Microbiology 2024Quote: ... 5 µM CHIR99021 (StemCell technologies) and 1.25% V/V DMSO) ...