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Citations for Stemcell Technologies :
401 - 450 of 9441 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated into single-cell suspension using Accutase and re-seeded onto fresh Poly-L-Ornithine / Laminin coated 12-well plates at a density of 150,000 cells in STEMdiff Neuron Maturation Kit medium (Stemcell Technologies) and medium exchanged every other day until days 14 and 21.
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bioRxiv - Developmental Biology 2024Quote: ... NSC suspensions were seeded at 200,000 cells per well in STEMdiff Neuron Differentiation Kit medium (Stemcell Technologies) and medium replaced every other day until day 7 ...
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bioRxiv - Developmental Biology 2024Quote: ... in motor neuron differentiation medium with 3 uM CHIR99021 (Stemcell Technologies) and cultured for 2 days to form neurospheres ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were plated for single cell clones by plating 1000 cells per 10cm2 dish on Synthemax with 1x CloneR (Stemcell technologies #05888), and single cell clones were genotyped by PCR for correct integration before sequencing both alleles from positive clones.
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bioRxiv - Developmental Biology 2024Quote: ... in APEL 2 base medium (Stemcell Technologies). On day 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... and fed at minimum every other day with mTeSR-Plus medium (STEMCELL Technologies) (Ludwig et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 μl DNase (1 mg/ml Stemcell Technologies) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Nodal-mCitrine and Nodal Knockout cell lines were cultured in mTeSR Plus (STEMCELL Technologies). All experiments were performed with cells of passage no later than 50 ...
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bioRxiv - Developmental Biology 2024Quote: ... we added 1 uM LDN-193189 (STEMCELL Technologies, 72147), 100 ng/ml BMP4 (R&D ...
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bioRxiv - Developmental Biology 2024Quote: ... in E8 medium (StemCell Technologies) and passaged using standard protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 μM ROCK inhibitor Y-27632 (Stem Cell Technologies, cat.# 72304; 10 mM stock in ddH2O), and 2.5 μM CHIR99021 (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2.5 μM CHIR99021 (Stem Cell Technologies, cat.# 72052 ...
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bioRxiv - Developmental Biology 2024Quote: ... and manually dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies) as previously described (Cardo et al ...
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bioRxiv - Genetics 2024Quote: ... coated plastic dishes and maintained in mTeSR1 medium (Stemcell Technologies, Canada, #05851), which was exchanged daily.
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bioRxiv - Genetics 2024Quote: ... The dissociation of H9 cells into clusters for subculturing was facilitated using ReLeSR (Stemcell Tech., 05873). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... T cells were plated at 1 million cells/mL in either ImmunoCult XF T cell Expansion Medium (STEMCELL Technologies) supplemented with 200U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
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bioRxiv - Immunology 2024Quote: ... cells harvested from the LEM cultures or xenografts were washed twice in Dulbecco’s phosphate-buffered saline (PBS, STEMCELL Technologies) to remove residual serum ...
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bioRxiv - Immunology 2024Quote: ... Coulombel (INSERM U362) were maintained in DMEM with 4,500 mg/L D-Glucose (STEMCELL Technologies) and 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... The RBCs in the samples were then lysed in cold Ammonium Chloride solution (STEMCELL Technologies) and the cells then passed through 40 μm filters and stained with antibodies in HBSS + 2% FBS ...
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bioRxiv - Immunology 2024Quote: ... Fresh samples were first processed using Lymphoprep™ (STEMCELL Technologies) to separate low-density fractions from granulocytes and erythrocytes ...
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bioRxiv - Immunology 2024Quote: ... Aliquoted CD34+ cell-enriched samples were cryopreserved in FBS (STEMCELL Technologies) containing 10% DMSO (Origen Biomedical) ...
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bioRxiv - Immunology 2024Quote: ... Positive selection of CD34+ cells was then performed on the low-density cells using the EasySep™ kit from STEMCELL Technologies) to obtain final CD34+ purities ...
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bioRxiv - Immunology 2024Quote: ... cells were rapidly thawed at 37 °C in a water bath and added dropwise into IMDM (STEMCELL Technologies) containing 10% FBS and 10 μg/mL DNase (MilliporeSigma) ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... per manufacturer’s instructions (STEMCELL Technologies, Vancouver). Purity (typically >85% ...
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bioRxiv - Genomics 2024Quote: ... cells were grown in Neurocult NS-A Proliferation Media (StemCell) supplemented with 0.0002% heparin (StemCell Technologies), EGF (20 ng/ml) ...
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bioRxiv - Genomics 2024Quote: ... CD3+ T cells were isolated by magnetic bead separation (StemCell Technology) from PBMCs of three leukopaks (Cambridge Biosciences ...
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bioRxiv - Genomics 2024Quote: ... Approximately 45 mg of flash-frozen skin or/fin was placed in a 6-well tissue culture plate (Stem Cell Technologies, cat. no. 38015) with 1 mL of TST buffer composed of 2 mL of 2X ST buffer ...
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bioRxiv - Genomics 2024Quote: ... using bead separation (Stemcell Technologies) and cryopreserved ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 10 vol% conditioned medium containing Rspondin1 (produced from HA-R-Spondin1-Fc HEK293T cell line ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 3 µM CHIR9902 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). 24 h after transfection ...
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bioRxiv - Genomics 2024Quote: ... 24 x 106 SNRPN-2A-GFP VP64dCas9VP64 (maternal) or dCas9KRAB (paternal) iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850 ...
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bioRxiv - Genomics 2024Quote: ... 1.7 x 106 matSNRPN-2A-GFP Tet1cdCas9 or VP64dCas9VP64 iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850 ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼430-fold coverage of the gRNA library ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼580-fold coverage of the gRNA sub-library ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Genomics 2024Quote: ... 250 µL of ImmunoCult Human CD3/CD28 T Cell Activator Mix (StemCell Technology) was added to the cells and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: CD3+ T cells were isolated from healthy donor leukopaks (Stemcell Technologies) using bead separation (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Genomics 2024Quote: ... and 1 x CC100 (StemCell Technologies) consisting of cytokines SCF ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 20% BITS 9500 (StemCell Technologies), 2 mM l-glutamine (Gibco) ...