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Citations for Stemcell Technologies :
401 - 450 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the control antibody was added (0.2 µg/µl, 1:3390, STEMCELL Technologies, 60070AD). Antibodies were incubated for 10 min at 4 °C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei suspension was mixed with 250 µl 50 % Iodixanol (60 % stock from STEMCELL Technologies, 7820), 1 x protease inhibitor (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2024Quote: ... cells growing at 60% confluence were transferred to a hypoxia chamber (STEMCELL technologies) which was purged with a gaseous mixture of 1% O2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and differentiation protocol as reported.10 At day -1 iPSC were prepared in mTeSR™1 (StemCell Technologies Cambridge, MA) and plated at approximately 40 colonies per well on Matrigel pre-coated wells ...
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bioRxiv - Molecular Biology 2024Quote: ... Macrophages were generated from iPSC using the STEMdiff™ Hematopoietic Kit (StemCell Technologies Cambridge, MA) and differentiation protocol as reported.10 At day -1 iPSC were prepared in mTeSR™1 (StemCell Technologies Cambridge ...
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bioRxiv - Neuroscience 2024Quote: ... human recombinant shh (C24II) (cat# 78065.1) (all from STEMCELL Technologies, Tullamarine, VIC, Australia). See table S2 for antibodies used for ICC.
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bioRxiv - Neuroscience 2024Quote: ... rhEGF (10 ng/ml, Stem Cell Technologies #E9644), bFGF (10 ng/mL ...
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bioRxiv - Neuroscience 2024Quote: ... GBM cells were grown in serum-free Neurocult NS-A Basal medium (Stem Cell Technologies #05750) that is supplemented with 2mM L-Glutamine (Wisent #609—65-EL) ...
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bioRxiv - Neuroscience 2024Quote: ... bFGF (10 ng/mL, Stem Cell Technologies #02634), N2-hormone mix (Thermo Fischer ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation into small aggregates with ReLeSR (StemCell Technologies, Vancouver, Canada) every 5–7 days and split in a 1:5 ratio into fresh Matrigel-coated dishes ...
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bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
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bioRxiv - Neuroscience 2024Quote: ... Neural Rosette Selection Reagent (Stem Cell Tech) was added to the culture for 1 h at 37°C for NPC selection ...
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bioRxiv - Neuroscience 2024Quote: ... early passages of NPCs were frozen in Cryostore CS10 (StemCell Technologies) for later usage.
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bioRxiv - Neuroscience 2024Quote: ... and the STEMdiff™ Cerebral Organoid Maturation Kit (Stemcell technologies, #08571), following the producer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... 70,000-80,000 EPSCs were used for the development of individual organoids using the STEMdiff™ Cerebral Organoid Kit (Stemcell technologies, #08570) and the STEMdiff™ Cerebral Organoid Maturation Kit (Stemcell technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... GBOs cultured for more than one month were dissociated into a single cell with accutase at 37 °C for 30 min and seeded into aggrewell 800 (Stemcell technologies) at a density of 9 × 105 cells/mL with 1 mL of GBO medium supplemented with 10 µM Y27632 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells are resuspended in N2B27 medium supplemented with ROCK inhibitor and seeded at a density of approximately 75 cells per microwell in 400 µm AggreWell plates (Stem Cell Technology, cat# 34425). Once cell structures appear solid ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2024Quote: ... Human CD34+ cells were magnetically enriched with the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies) from either normal BM or CB samples ...
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bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of StemSpan SFEM (Stem Cell Technologies) supplemented with 50ng/ml Stem cell Factor (SCF ...
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bioRxiv - Cell Biology 2024Quote: ... All dissociation reagents were obtained from STEMCELL Technologies. For flow cytometry analysis ...
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bioRxiv - Cell Biology 2024Quote: ... seeded 18 hours prior in a 6-well plate with 1 mL of mTESR1 (StemCell Technologies 85850) or MEM medium ...
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bioRxiv - Cell Biology 2024Quote: ... Guided differentiation into forebrain neurons can be induced by switching to neuronal medium (BrainPhys (STEMCELL Technologies, 05792), 1× N2 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... neural rosettes were selected by STEMdiff Neural Rosette Selection Reagent (Stemcell technologies, 05832). Maintenance of neural progenitor cells was performed in neural maintenance medium with 10 µM bFGF (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... macrophage precursors were harvested using a cell strainer (Stemcell technologies, 27250). Macrophage precursors were added to the NPC culture in 1:1 neural maintenance medium:microglia medium ((DMEM-F12+Glutamax ...
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bioRxiv - Cell Biology 2024Quote: ... blood was layered on top of Lymphoprep™ (StemCell Technologies, 07851) in a 15 mL tube and centrifuged at 800 rcf for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Whole blood was collected from 12M and 20M SM/J and BL6 mice via intracardiac puncture and diluted into an equal volume of Dulbecco’s PBS + 2% FBS (StemCell Technologies, 07905). According to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2024Quote: ... Splenocytes were dissociated from the tissue matrix by gentle compression through a 100 μm strainer (StemCell Technologies, 27217). RBC lysis (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... at a density of 10e4 cells/cm² in mTesR1 medium (Stemcell Technologies, 85850) and Rock inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Subculturing of iPSCs was performed with ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Cell Biology 2024Quote: We followed guidelines from Stem Cell Technologies for culturing human colonic organoids with some modifications ...
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bioRxiv - Cell Biology 2024Quote: Organoids were differentiated in the IntestiCult Organoid Differentiation Medium (StemCell Technologies, 100-0214) supplemented with 5 μM DAPT ((2S)-N-[(3,5-difluorophenyl ...
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bioRxiv - Cell Biology 2024Quote: ... The plates were incubated at 37°C for 20 min to allow the domes to solidify after which 750 μl of human intestinal organoid growth media (Human IntestiCult Organoid Growth Medium, hIOGM, StemCell Technologies, 06010) was added along with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cell Biology 2024Quote: ... Single-cell suspensions were then obtained from tissue organoids by serially dissociating with trypsin-EDTA (0.25%, STEMCELL Technologies), 5 mg/mL dispase (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg/mL dispase (STEMCELL Technologies), supplemented with 100 µg/mL DNase1 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: Esophageal biopsies were dissociated (27) and CD45+ cells were removed using positive isolation beads (STEMCELL TECHNOLOGIES). The untouched epithelial-enriched cell fraction was washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cell isolation was performed with magnetic separation (EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit, StemCell Technologies).
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bioRxiv - Immunology 2024Quote: ... the EasySep Human Cord Blood CD34 Positive Selection Kit II (STEMCELL Technologies) was used ...
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bioRxiv - Microbiology 2024Quote: ... and IntestiCult™ Organoid Growth Medium (OGM, StemCell Technologies), that was replaced every other day ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from blood of convalescent COVID-19 donors using the EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes or BM cells were enriched using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions from fluorescently labeled mice were mixed with methylcellulose-containing MethoCult M3534 (Stemcell Technologies) in various dilutions and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: PBMCs were isolated from HLA A2:01 specific healthy donor leukopaks (Stemcell Technologies) using a Stemcell isolation kit and cryopreserved ...
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bioRxiv - Genomics 2024Quote: ... Live tetramer positive cells were bulk sorted into CryoStor freezing medium (07959, STEMCELL Technologies) on a Sony SH800 sorter using a 100µm nozzle and frozen at –80 °C for single cell sequencing.
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bioRxiv - Genetics 2024Quote: Cryopreserved PBMCs were obtained from StemCell Technologies (Catalog # 70025). PBMCs were thawed and CD3+ T cells were isolated immediately by Pan T Cell Isolation Kit (Miltenyi # 130-096-535) ...
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bioRxiv - Genetics 2024Quote: ... hiPSC were cultured in mTeSR1 medium (StemCell Technologies) in a humidified incubator with 5% O2 ...