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Citations for Macherey-Nagel GmbH :
2651 - 2700 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... GSE were filtered through a glass-fiber frit (Chromabond, Macherey-Nagel, France), then frozen in liquid nitrogen and freeze-dried ...
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bioRxiv - Microbiology 2020Quote: ... Agarose Gel purification and DNA plasmid extraction kits were purchased from Macherey-Nagel.
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bioRxiv - Plant Biology 2020Quote: DNA extraction was performed using NucleoSpin® Plant II Mini (Macherey-Nagel, Dürin, Germany) as per manufacturer’s instructions with minor modifications ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... infiltrated leaves were harvested after 6 h for total RNA isolation with NucleoSpin® RNA Plus Kit (Macherey-Nagel). First strand cDNA was synthesized with RevertAid™ M-MuLV reverse transcriptase (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of the permeabilized sample was then processed using the Machery-Nagel NucleoSpin Virus kit (Macherey-Nagel, Catalog # 740983.50 and 740983.250).
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bioRxiv - Microbiology 2020Quote: ... DNase digestion was performed post-column binding (Macherey-Nagel, Catalog #740963) at room temperature for 5 minutes at a final concentration of 40 ng/μL ...
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bioRxiv - Microbiology 2020Quote: The total RNA was isolated from macrophages (infected and uninfected) using Nucleo spin RNA extraction kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Double-stranded DNA was purified using NucleoMag beads (Macherey-Nagel Inc., Bethlehem, PA) and subsequently subjected to random amplification with primer FR20RV prior to sequencing library preparation [20].
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bioRxiv - Systems Biology 2020Quote: RNA from blood was extracted using NucleoSpin RNA Blood Mini kit (Macherey-Nagel). Lysis buffer and proteinase K were added to the frozen pellet and shaken while thawing ...
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bioRxiv - Microbiology 2020Quote: ... The genomic DNA was extracted from intact stomachs using the NucleoSpin Tissue XS kit (Macherey-Nagel®). The integrity of the DNA was checked in a 1% agarose gel ...
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bioRxiv - Plant Biology 2020Quote: Total RNA of Botrytis infected leaves was extracted using the NucleoSpin RNA Plant kit (Macherey-Nagel, Germany). RNA samples were reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... in combination with the NucleoSpin RNA/DNA buffer set (Macherey-Nagel). DNA was first eluted from the column in 80 μL DNA elution buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples of 5 mL liquid culture were quickly harvested onto nitrocellulose membrane filters (ø 25 mm, 0.45 µm pore size, Porafil NC, Macherey-Nagel) by vacuum filtration ...
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bioRxiv - Microbiology 2020Quote: ... Purified rbcL amplicons (NucleoSpin® gel and PCR clean-up kit, Macherey-Nagel, France) were either Sanger-sequenced directly or cloned into pGEM-T easy vector plasmids using competent Escherichia coli JM109 cells (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was isolated from DH5α using the Nucleospin Plasmid kit (MACHEREY-NAGEL Inc., Bethlehem, PA) and transformed into the DAP-auxotroph donor strain E ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from N/Tert or HFK cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... followed by the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) and sent to Eurofins (Ebersberg ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted plant DNA of at least one diseased plant individual per site using the NucleoSpin® 96 Plant II kit (Macherey-Nagel, Germany). We obtained DNA for 134 out of the 171 diseased plant individuals ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted DNA from fresh spores stored at -20°C using the Nucleospin Soil Kit (Macherey-Nagel, Germany). Genomes were sequenced using Illumina 150 bp paired-end sequencing technology at 46X coverage on average ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated using NucleoSpin RNA XS (Macherey-Nagel, Germany), and the sequencing library was prepared with SMARTer Stranded Total RNA-Seq Kit v2-Pico Input (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified with Nucleospin (Macherey-Nagel) as per manufacturer’s instructions and sequenced with Sanger sequencing (Eurofins or Genscript).
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bioRxiv - Neuroscience 2020Quote: ... according to the manufacturer’s protocol (Macherey-Nagel) and on Zephyr automatic station (Perkin-Elmer) ...
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bioRxiv - Microbiology 2020Quote: ... NucleoSpin RNA extraction kit (Macherey-Nagel, #740955.50) was used following the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2020Quote: ... following which it was applied onto a pre-equilibrated (EQ buffer containing 0.15% Triton X-100) Nucleobond AX-100 column (Macherey-Nagel). The column-bound RNA was washed twice with wash buffer WB (10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... 1.25 µg/µl) purified with NucleoBond (Macherey-Nagel, Germany) in sterile PBS with 0.1% fast green dye was injected in the right lateral ventricle of each embryo using pulled borosilicate glass capillaries ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were purified with PCR clean-up kit from MACHEREY-NAGEL and eluted in 50 μL of elution buffer.
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bioRxiv - Cell Biology 2020Quote: PCR products were analyzed by agarose gel electrophoresis and purified using column purification (Macherey-Nagel).
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Plant Biology 2020Quote: DNA extractions were performed using NucleoSpin® Plant II Mini (Macherey-Nagel) as per manufacturer’s instructions with the following minor modifications ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleosil 100-5 N(CH3)2 column (Macherey-Nagel GmbH & Co, Düren, Germany) and eluted isocratically with 100% methanol containing 0.01% acetic acid at a flow rate of 1 ml min-1 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from newly matured leaves using a NucleoSpin RNA kit from MACHEREY-NAGEL according to the manufacturer’s instructions and then treated with DNase I ...
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bioRxiv - Microbiology 2020Quote: Total RNAs were extracted from cell-lysates using the NucleoSpin RNA II Kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA extraction was carried out using the NucleoSpin kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA genes of AOB and AOA was amplified with primers amoA-1F/amoA-2R (66 ...
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bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
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bioRxiv - Genomics 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin-RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was extracted on column using the NucleoSpin-RNA Kit (Macherey-Nagel). DNaseI treatment was done directly on the column ...
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bioRxiv - Microbiology 2020Quote: ... The pH was monitored using indicator stripes (Macherey-Nagel) and a pH meter (WTW ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 50µl aliquots of supernatant using the Nucleospin 96 virus kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions (Macherey-Nagel). RNA reverse transcription was performed using SuperScript III (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using Nucleospin PCR clean-up kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl pH 8.5) ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted using NucleoSpin RNA kit (MACHEREY-NAGEL). MCF10A libraries from three biological replicates of each cell type were prepared using NEBNext® Ultra(tm ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated using the Nucleospin RNA kit (Macherey-Nagel) and quantified using the Qubit RNA Assay kit (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... and RNA purification using NucleoSpin® RNA Clean-up (Macherey-Nagel, Germany). Ribosomal RNA depletion was performed using the RiboMinus Plant Kit for RNA-Seq (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated using a NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA (gDNA) was isolated from mouse ear punches and hepatocytes with the NucleoSpin Tissue Kit (Macherey-Nagel, Germany). RNA was isolated from mouse liver with peqGOLD RNAPure reagent (VWR ...