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Citations for Macherey-Nagel GmbH :
2751 - 2800 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Lipids were analysed by Thin Layer Chromatography (TLC) on silica gel-coated plates (G-60, 0.25 mm thickness, Macherey-Nagel) developed with CHCl3/CH3OH/H2O (65:25:4 ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... and Nucleospin® RNA kit (Macherey-Nagel) according to the manufacturers’ protocol.
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bioRxiv - Microbiology 2020Quote: ... The tips of the swabs were place in 0.5 mL of RA1 lysis buffer (Macherey-Nagel, Ref. 740961) supplemented with 1% β-mercaptoethanol and vortexed ...
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bioRxiv - Microbiology 2020Quote: ... Extraction of total cellular RNA was done using Nucleospin® RNA Plus kit (Macherey-Nagel) according to the manufacturers’ instruction.
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from homogenized organs using the NucleoSpin™ Kit Plus (Macherey-Nagel), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Spike peptide-stimulated splenocytes split were used for RNA extraction by using the sNucleoSpin™ Kit Plus kit (Macherey-Nagel). cDNA was generated by using a high-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... ii) Nucleomag Vet kit (Macherey-Nagel) or MagAttract 96 cador Pathogen Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... or 50µl of serum using the NucleoSpin kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The NucleoSpin RNA extraction kit (Macherey-Nagel) was used to isolate total intracellular RNA according to the manufacturer’s specification ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA (containing viral genomic RNA) was extracted 48 h after transfection using NucleoSpin RNA Plus kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
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bioRxiv - Genomics 2020Quote: RNA was extracted using one of three kits (Qiagen QIAamp Viral RNA Mini kit, Macherey-Nagel Nucelospin Virus Mini kit ...
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bioRxiv - Cancer Biology 2020Quote: ... ovarian carcinoma cells had their RNA chemically purified using the Nucleospin RNA Plus kit (Macherey-Nagel: 740984.50) as recommended by the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The overnight culture was used to inoculate a 500 mL LB with the antibiotic and the plasmid DNA was isolated using NucleoBond Xtra Midi EF (Macherey-Nagel, Dueren, Germany). Sequence flanking the spacer sequences were amplified and were sequenced ...
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bioRxiv - Immunology 2020Quote: ... Removal of dsRNA contaminants from 100 to 500 μg IVT mRNA was performed using microcentrifuge spin columns (NucleoSpin Filters, Macherey-Nagel, Düren, Germany), cellulose fibers (C6288 ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissue using a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers suggested by the stock facility (forward ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL was applied on a TLC plate (ALUGRAM® Xtra SIL G UV254, Macherey-Nagel). For the mobile phase CHCl3/MeOH in a ratio of 9:5 (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed on a GC2010 gas chromatograph (Duisburg, Shimadzu) equipped with an OPTIMA 17ms column (Macherey-Nagel, Düren, Germany) with a linear gradient starting at 250 °C and heating with 10 °C min−1 until 300 °C ...
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bioRxiv - Biochemistry 2020Quote: Preliminary product identification was performed with a gas chromatograph - mass spectrometer (GC-MS-QP2010S, Shimadzu, Germany) equipped with an OPTIMA 17ms column (Macherey-Nagel, Germany) using a linear temperature gradient starting at 250 °C and heating with 10 °C min−1 to 300 °C ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from ground tissues using the NucleoSpin RNA Plant extraction kit (Macherey-Nagel). Non-strand-specific cDNA libraries of 250-300 bp were synthesized and sequenced by Novogene (Hong Kong ...
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bioRxiv - Microbiology 2020Quote: ... followed by filtration (MN 615 ¼, Macherey-Nagel, Dueren, Germany) and centrifugation (1,400×g ...
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bioRxiv - Molecular Biology 2020Quote: ... or NucleoSpin ® RNA Clean-up (Macherey-Nagel) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... A modified protocol for purification of RNA from solution (Macherey-Nagel) was used for the cytosolic fraction ...
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bioRxiv - Molecular Biology 2020Quote: ... Both the fractions were subjected to RNA purification with NucleoSpin ® RNA Clean-up (Macherey-Nagel) and equal volumes of eluate were used for cDNA production and real time RT-qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cultures using NucleoBond Plasmid MAXI KIT (Macherey-Nagel) and resuspended in TE buffer ...
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bioRxiv - Microbiology 2020Quote: ... the NucleoSpin kit (Macherey-Nagel) was used ...
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bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: Following stimulation cells were lysed and total RNA isolated using Nucleospin RNA purification columns according to the manufactures protocol (Macherey-Nagel). 0.5 to 1 µg of total RNA was reverse transcribed using BioRad iScript ...
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bioRxiv - Immunology 2020Quote: ... gDNA were extracted with NucleoSpin® Blood kit (Macherey-Nagel) and TCR sequencing was performed by Adaptive BiotechnologiesR (Seattle ...
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bioRxiv - Pathology 2020Quote: We isolated total RNA Nucleospin RNA kit (Macherey-Nagel) with DNase I treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Individual quenched timepoints were spotted PEI-TLC plates (Macherey-Nagel) and nucleotides were resolved in 1.5 KH2PO4 pH 3.5 buffer ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel, Gutenberg, France). One microgram of total RNA was reverse-transcribed into cDNA using High Capacity cDNA Reverse Transcription Kit (Applied biosystems ...
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bioRxiv - Physiology 2020Quote: ... NucleoSpin Tissue DNA purification kit (Macherey-Nagel, Dueren, Germany) was used to extract total DNA from adipose tissue according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: Standard methods were used to determine the TSS and volatile suspended solids concentration.25 The COD was measured using test kits (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... and a 400bp band was extracted using the NucleoSpin PCR Cleanup Kit (Macherey-Nagel, 740609.250). The concentration of DNA in the resulting eluent was determined via a Qubit 2 fluorometer (Thermofisher) ...
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bioRxiv - Bioengineering 2020Quote: ... DNA was extracted with the NucleoSpin®Soil Kit (MACHEREY-NAGEL GmbH & Co. KG, Germany) using buffer SL2 and enhancer solution ...
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bioRxiv - Molecular Biology 2020Quote: ... or NucleoSpin® RNA Clean-up (Macherey-Nagel) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... or NucleoSpin® RNA Clean-up (Macherey-Nagel) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted with Nucleospin® RNA isolation kit (Macherey-Nagel GmbH & Co.) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted with Nucleospin® RNA isolation kit (Macherey-Nagel GmbH & Co. Düren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: Total RNA was extracted from 2.3×106 Sf9 cells in each sample using NucleoSpin® RNA kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the PCR products purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Indexing PCR and NGS were performed by Microsynth (Balgach) ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was isolated using NucleoSpin RNA XS (Macherey-Nagel, 740902). RNA Integrity Number (RIN ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...
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bioRxiv - Genomics 2020Quote: ... Cleaved Histone-DNA complexes were isolated by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel, 740609).
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bioRxiv - Molecular Biology 2020Quote: ... and total RNA was isolated using NucleoSpin RNAXS kit (Macherey-Nagel) according to the user manual ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a trimethyl-silylation using N-Methyl-N-trimethylsilyl-trifluoracetamid (MSTFA, Macherey-Nagel). Dried samples were re-suspended in 5 µL of a freshly prepared (20 mg/mL ...
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bioRxiv - Zoology 2020Quote: Approximately 50-100 mg of tissue (from the second pereopod) was used for DNA extraction using the NucleoSpin® Tissue Kit (Macherey-Nagel) following the instructions of the manufacturer ...