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Citations for Macherey-Nagel GmbH :
2451 - 2500 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... RNA samples were DNase treated (Macherey-Nagel, Düren, Germany) to remove genomic DNA that could result in a false positive pfs25 signal (Meerstein-Kessel et al. ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the pathogen Nucleic Acid Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in 50 μL elution buffer ...
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REGN-COV2 antibody cocktail prevents and treats SARS-CoV-2 infection in rhesus macaques and hamstersbioRxiv - Microbiology 2020Quote: ... and the NucleoMag Pathogen kit (Macherey-Nagel). Extraction controls were prepared with each batch of samples ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted by phenol-chloroform extraction followed by column clean-up using NucleoSpin® DNA Plant II kit (Macherey-Nagel). Clustering and DNA sequencing of wild-type ...
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bioRxiv - Microbiology 2020Quote: ... The obtained RNA samples were cleaned-up using NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel). RNA libraries were prepared for sequencing using standard Illumina protocols and paired-end sequence reads were generated using the Illumina MiSeq system (BaseClear BV ...
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were then purified with a PCR clean-up kit (Macherey-Nagel, DE), quantified with the PicoGreen assay as described above ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from shoots and roots using NucleoSpin RNA Kit (MACHEREY-NAGEL). 500 ng total RNA was reverse-transcribed using PrimeScript RT Master Mix (Perfect Real Time ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified to remove unincorporated nucleotides and primers using NucleoFast® 96 PCR plates (Macherey-Nagel, Oensingen, Switzerland). Details of the annealing temperatures and primers were published previously (Friesen et al ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with NucleoSpin RNA Clean-up kit (Macherey-Nagel). RNA was quantified by Nanodrop (Thermoscientific) ...
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bioRxiv - Microbiology 2020Quote: ... coli using NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, Germany). Deletion of the degU ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from tobacco leaf disks (sampled from glasshouse grown plants and quickly frozen in liquid nitrogen) using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Fisher Scientific, UK). cDNA was synthesized using 1 μg total RNA in 20 μl using the oligo-dT primer according to the protocol in the RevertAid Reverse Transcriptase kit (Fermentas ...
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bioRxiv - Microbiology 2020Quote: ... falciparum genomic DNA was isolated using NucleoSpin® Blood Columns (Macherey-Nagel) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... equipped with a 250/10 Nucleodur C18 Pyramid 5M column (Macherey-Nagel) and a C18 pre-column ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from cells or supernatants using the NucleoSpin RNA extraction kit (Macherey-Nagel) according to the manufacturer’s specification ...
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bioRxiv - Genetics 2020Quote: ... the DNA was purified using NucleoSpin® columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Biophysics 2020Quote: ... both were cleaned up with a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Bethlehem, PA). Shaker and CiVSP cRNAs were then synthesized from linearized DNA using the mMESSAGE mMACHINE T7 or SP6 transcription kit ...
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bioRxiv - Systems Biology 2020Quote: The viral RNA from supernatant of infected cells was isolated using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted using the Nucleobond Xtra Maxi kit according to the manufacturer’s protocol (Macherey-Nagel) and digested with NdeI (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... in combination with the NucleoSpin RNA/DNA buffer set (Macherey-Nagel). DNA was first eluted from the column in 100 µL DNA elution buffer and subsequently the column was treated with recombinant DNase I (20 units/100 µL ...
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bioRxiv - Microbiology 2020Quote: ... NucleoBond Xtra Midi Kit (Macherey-Nagel) and QIAamp DNA Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from primary keratinocytes cells using the NucleoSpin RNA kit (Macherey-Nagel) followed by cDNA preparation (iScript cDNA synthesis kit ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated with the NucleoSpin RNAII kit (Macherey-Nagel, Oensingen, Switzerland).
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated using the Nucleospin RNA (Macherey-Nagel) with on column DNase treatment ...
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bioRxiv - Cell Biology 2020Quote: RNA was purified from cerebellum samples from Cockayne patients and age- and gender-matched healthy individuals by using the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extracted using the NucleoSpin RNA Kit (Macherey-Nagel) was reverse transcribed into cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... after which plasmids were isolated using the NucleoBond Xtra Midi Plus Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel), lyophilized ...
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bioRxiv - Cell Biology 2020Quote: ... Cholesterol was derivatized from dried FAME fraction by first dissolving in 20µL pyridine and then adding 30 µL N-methyl-N-trimethylsilyl-trifluoroacetamide (Macherey-Nagel) at 37°C for 30 minutes.
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA from cells of interest was isolated using the NucleoSpin RNA Kit from Macherey-Nagel (Düren, Germany) according to manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Cell Biology 2020Quote: ... Maxi kit (Macherey-Nagel, Düren, Germany). Reverse transcription was performed with the iScript cDNA Synthesis kit (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... and RNA was isolated using a Nucleospin RNA isolation kit (Macherey-Nagel, Germany). Agarose gel analysis was used to assess RNA quality ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from isolated islets from 2 mice per genotype with Nucleospin RNA plus XS (Macherey-Nagel). Complementary DNA was generated and linearly amplified from 3 ng total RNA using the Ovation RNA-seq V2 system (NuGEN technologies Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... were purified using an endonuclease free plasmid extraction kit (NucleoBond Xtra Maxi Plus EF, Macherey-Nagel, cat# 740426.10).
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bioRxiv - Genomics 2020Quote: Total RNA was extracted using NucleoSpin® RNA (Macherey-Nagel). CAGE libraries were prepared as previously described ...
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bioRxiv - Biochemistry 2020Quote: ... using a Nucleodur HTec C18 VP 125/10 semi-preparative column (Macherey-Nagel, Germany), with an average particle diameter of 5 μm and pores 100 Å and a geometry of 150 × 3.0 mm ...
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bioRxiv - Biochemistry 2020Quote: ... using Nucleodur HTec C18 VP 125/10 column (Macherey-Nagel, Germany) with average particle diameter of 5 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... Small and large RNA were extracted from frozen samples using NucleoSpin miRNA kit (Macherey-Nagel; 740971.50). 1 ug RNA was mixed with 0.025 pmole of tRNA standards (e.coli-tyr-tRNA and s.cerevisia-phe-tRNA at ration of 1:8 (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... We extracted total RNA from entire nymphs following the NucleoSpin RNA (Macherey-Nagel Inc.) protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flowers and whole chili pepper fruits at different developmental stages using a NucleoSpin(tm) RNA Plant kit (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Total mRNA was extracted from pools of 30 embryos using NucleoSpin RNA kit (Macherey-Nagel, Bethlehem, PA, USA). Complementary DNA (cDNA ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from cells using NucleoSpin kits (Macherey-Nagel, Bethlehem, PA, USA). One microgram of total RNA was used for q-PCR analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... using NucleoSpin® RNA kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... and small (<200 nt) and large RNA (>200 nt) were extracted in separate fractions using the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany) following the manufacturer’s recommended protocols ...
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bioRxiv - Genomics 2020Quote: DNA extractions were performed employing the NucleoSpin® Plant II Plant Mini kit (Macherey-Nagel). Quantification of the isolated DNAs was performed using NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Total RNA from infected plant samples were extracted using NucleoSpin RNA extraction kits (Macherey-Nagel) as described in (Sucher et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified PCR products were gel-purified with a gel extraction kit (Macherey-Nagel GmbH, Dueren, Germany). The inhibitory unit (iUnit ...