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Citations for Macherey-Nagel GmbH :
2901 - 2950 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were extracted by using the NucleoSpin® Plasmid Miniprep Kit (Macherey-Nagel). All extracted plasmids were sent for DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... Samples were size-selected using NucleoMag NGS Clean-up and Size Select (Macherey-Nagel) to 50 uL final volume ...
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bioRxiv - Cell Biology 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted on column using the NucleoSpin RNA Kit (Macherey-Nagel). DNase I treatment was done directly on the column ...
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bioRxiv - Biochemistry 2020Quote: ... 80 μL N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA, Macherey-Nagel) was added and the samples were incubated at 37 °C for 30 min (1.200 rpm) ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from cells using the NucleoSpin RNA mini kit (Macherey-Nagel) with on-column deoxyribonuclease treatment ...
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bioRxiv - Genomics 2020Quote: ... DNA was purified with PCR clean up columns (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and sequenced using Illumina technology.
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bioRxiv - Genomics 2020Quote: ... or NucleoSpin Blood Kit (Macherey-Nagel, Germany). SV breakpoints were confirmed with Sanger Sequencing where possible ...
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bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... PCR products were gel-purified using a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel 740609) followed by Sanger sequencing ...
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bioRxiv - Immunology 2020Quote: ... The PCR product was purified using the PCR Clean-up Kit (Macherey-Nagel) and was subsequently digested with PspXI (New England BioLabs ...
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bioRxiv - Immunology 2020Quote: ... was expressed in 293T cells from a plasmid kindly provided by Florian Krammer and purified using Ni-NTA Agarose (Macherey-Nagel), as previously published (Stadlbauer et al. ...
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bioRxiv - Immunology 2020Quote: ... DNA was isolated using a maxi-prep kit (NucleoBond® Xtra Maxi, Macherey-Nagel) and sequence confirmed.
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bioRxiv - Microbiology 2020Quote: Total DNA was extracted using the NucleoSpin® Soil kit (Macherey-Nagel, Germany) by disrupting the cells by bead beating (30 s at 5.5 m s-1) ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... to reach at least 200x colonies per guide-RNA and the library plasmid pool was purified using NucleoBond Xtra-Maxi Kit (Macherey-Nagel, #740414.50). Then ...
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bioRxiv - Microbiology 2020Quote: ... using the NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel, Germany).
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bioRxiv - Microbiology 2020Quote: Lung left inferior lobes from hamsters were cut into pieces and lysed using the RA1 lysis buffer provided with the NucleoSpin® RNA Plus kit (Macherey-Nagel). RNA extraction was then performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA concentration was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified using Nucleo Spin Gel and PCR Clean up (Macherey-Nagel, Germany). The cleaned-up PCR products were sent Macrogen (Europe ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using the NucleoSpin DNA Insect Kit (Macherey-Nagel) with NucleoSpin Bead Tubes Type E and MN Bead Tube Holder in combination with the Vortex-Genie 2 ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from the cell pellets using the NucleoBond AXG20 kit (Macherey-Nagel), and DNA purity and concentration were determined with the Nanodrop 2000c spectrophotometer (Thermoscientific).
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bioRxiv - Molecular Biology 2020Quote: ... or a NucleoSpin RNA extraction kit (Macherey-Nagel), treated with Turbo DNase (Ambion) ...
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bioRxiv - Microbiology 2020Quote: ... difficile strains was estimated by quantitative PCR on genomic DNA extracted using the NucleoSpin Microbial DNA kit (Macherey-Nagel). The total chromosome copy number was quantified based on the reference gene dnaF (CD1305 ...
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bioRxiv - Immunology 2020Quote: ... stained with ethidium bromide and purified with NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Germany). Purified PCR products were ligated in pGEM-T easy vectors (Promega ...
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bioRxiv - Immunology 2020Quote: ... One selected transformant of each construct was grown to obtain plasmid DNA (Miniprep kit, Macherey-Nagel). All constructs were verified by Sanger DNA sequencing with T7 and SP6 primer sets (GATC Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Total DNA was extracted from cell pellets using NucleoSpin Tissue (Macherey-Nagel) kit ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was isolated using NucleoSpinRNA XS kit (Macherey-Nagel, Dueren, Germany). Eluted RNA was subject to RT-qPCR or RNA sequencing for the detection of enriched transcripts.
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: RNA was isolated from MEFs or NIH3T3 cells using the Nucleospin RNA plus kit (Macherey-Nagel, 740984.5). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was purified with NucleoBond (Macherey-Nagel, Germany). 2A encodes for a ribosomal skip sentence ...
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bioRxiv - Molecular Biology 2020Quote: ... NucleoBond Xtra Midi kit (Macherey-Nagel; 740412.50) was used.
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), quantified with a Qubit Fluorometer and Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... DNA was purified by using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL). The transposed DNA was amplified by PCR for 10-12 cycles by using the Nextera DNA Library Preparation Kit and Nextera XT Indexing Kit (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from whole blood samples using the NucleoSpin 96 RNA tissue kit protocol (Macherey-Nagel) with some modifications as previously described15.
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bioRxiv - Immunology 2020Quote: OMM bacteria were grown as described and 1 mL of each bacterial culture supernatant was homogenized using NucleoSpin Bead Tubes (Macherey-Nagel, Düren, Germany) and a Precellys Evolution Homogenizer (Bertin Corp. ...
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bioRxiv - Genomics 2020Quote: ... DNA was isolated from 1.5 ml tank milk using the NucleoMag kit (Macherey-Nagel). The tank milk DNA was first genotyped using the Illumina LD array interrogating 17K SNPs ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel, Hoerdt, France) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from the infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel) and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... or NucleoSpin® Plasmid EasyPure Miniprep Kit (Macherey-Nagel, USA) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... The reactions were then run at 100V in a 1% agarose gel and the digest vectors were excised and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, USA) according to instructions set by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... Colonies were sequence confirmed and then maxi-prepped (NucleoBond® Xtra Maxi, Macherey-Nagel). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at −20 °C.
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bioRxiv - Microbiology 2020Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2020Quote: ... Protein was purified by SPE with a Chromabond SPE-C4 cartridge (Macherey-Nagel, Germany). Briefly ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from 6 pools of 50 worms using an RNeasy® kit (Macherey-Nagel). Total RNA was used for oligo(dT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The leaf material was lysed with sterile sand and 7mm glass beads before DNA was extracted with the NucleoMag 96 plant kit (Macherey-Nagel Gmbh & Co., Düren, Germany) using the KingFisher Flex magnetic particle processor (Thermo Scientific) ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using NucleoMag plant kit extraction (Macherey-Nagel, Germany) in a KingFisherTM Flex system (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Genetics 2020Quote: ... RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point of DNA using primers flanking the guides (primers 5’-AGGGGTTGAGCTGGATTCC-3’ and 5’-CCAGCACCCACTCTGTAGAT-3’ ...
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bioRxiv - Genomics 2020Quote: ... We extracted genomic DNA using NucleoSpin Microbial DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel). Genomic DNA for strain Y55 was extracted independently using the QIAGEN Blood & Culture DNA Midi Kit ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied onto a C-18 column (CHROMABOND C-18 ec, Macherey-Nagel, Düren, Germany) equilibrated with 5 mL 100% (v/v ...