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Citations for Macherey-Nagel GmbH :
2701 - 2750 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel, Oensingen, Switzerland) from which the nucleotide sequence was determined using Sanger sequencing (Microsynth ...
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bioRxiv - Microbiology 2021Quote: ... followed by rapid filtration through membrane filters (MN gf-5 0.4 μm; Macherey-Nagel). The filters were dissolved in 5 ml of scintillation fluid (MP Biomedicals) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from lungs or infected cells using NucleoSpin® RNA kit (Macherey-Nagel) and reverse transcribed using the iScript™ Reverse Transcription Supermix for RT-qPCR kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: RNA was purified from cells using the NucleoSpin RNA Mini Kit (Macherey-Nagel. 740955.50). 1ug of RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (BioRad 1725034 ...
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bioRxiv - Molecular Biology 2021Quote: ... were purified with NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel), and quantified with the Quant-It PicoGreen double stranded DNA Assay kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were then purified using NucleoSpin®Gel and PCR Clean up kit (Macherey-Nagel™). Final elution product was divided in two for sequencing by Sanger (after cloning ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. Genomic DNA extractions from Amycolatopsis and E ...
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bioRxiv - Plant Biology 2021Quote: ... then loaded into two properly conditioned SiOH SPE cartridges (500 mg, MACHEREY-NAGEL). The cartridges were then washed with 2 ml n-hexane ...
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bioRxiv - Immunology 2021Quote: ... total protein was isolated by NucleoSpin TriPrep kit (Macherey-Nagel) from 5×106 PMNs ...
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bioRxiv - Immunology 2021Quote: ... All protein concentrations were determined with the MN Protein Quantification Assay (Macherey-Nagel). Western blotting was performed utilizing AnykD Mini-PROTEAN TGX Gels (Biorad ...
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bioRxiv - Immunology 2021Quote: ... RNA from cultured ILC2s was extracted using a NucleoSpin RNA Kit according to manufacturer’s instructions (MACHEREY-NAGEL). The concentration and purity of extracted RNA was measured using a Spectrophotometer NanoDrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... it was purified and concentrated using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel). The concentration of mRNA was measured with the QubitTM RNA BR Assay Kit and then stored at - 80⁰C in MilliQ water until use.
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bioRxiv - Immunology 2021Quote: ... and purified with NucleoBond® Xtra Midi/Maxi (Macherey-Nagel). T2 were retrovirally transduced with the HLA restriction elements HLA-A03:01 and B27:05 as described before (13) ...
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bioRxiv - Microbiology 2021Quote: ... selected clones were grown overnight at 37°C under agitation and the plasmid DNA was extracted and purified using the NucleoSpin plasmid Mini or Midi kit (Macherey-Nagel).
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from flow-sorted naive CD8+ T cells (n = 300 per condition) using a NucleoSpin RNA XS Kit (Macherey-Nagel), and cDNA was synthesized using Reverse Transcription Master Mix (Fluidigm) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was prepared with NucleoSpinR RNA mini isolation kit (MACHEREY-NAGEL, GmbH&Co.KG, Cat#740955), and RT-qPCR was carried out using HA273 and HA274 primers for rtTA amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was extracted as pooled libraries using NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Duere, Germany) and stored at −20 ℃.
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Molecular Biology 2021Quote: Complementary DNAs were obtained by reverse transcription of total RNA using the NucleoSpin RNA/Protein kit (Macherey-Nagel) and used as templates for PCR amplification using Brilliant III Ultra-Fast QPCR Master Mix (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted from the samples using the NucleoSpin® DNA Food kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from 100 freshly harvested seeds in three biological replicates using the NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted using a NucleoSpin kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted usingNucleospin® RNA XS kit (Macherey-Nagel, Duren, Germany). cDNA was then generated using Fluidigm Reverse Transcription Master Mix (Fluidigm) ...
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bioRxiv - Microbiology 2021Quote: ... RNA extraction was performed from 50 μl culture supernatant with the viral RNA kit (Macherey-Nagel) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Solid phase extraction was carried out using Chromabond SiOH cartridges filled with 2 g of unmodified silica gel (Macherey-Nagel, Germany). Chemicals ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel). 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and then filtered through pre-baked (550< 8 hours) quartz fibers (47 mm, 0.3um pore size, Macherey-Nagel QN-10) using pre-baked filter stands (EMD Millipore).
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bioRxiv - Microbiology 2021Quote: ... Solid phase extraction cartridges (Chromabond easy, Macherey-Nagel, Düren, Germany) were equilibrated with 4 mL of methanol (Chromasolv© Plus ...
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bioRxiv - Microbiology 2021Quote: A NucleoBond Xtra midi kit (Macherey-Nagel) and a QIAamp DNA minikit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Bodies were homogenized individually in 400 μl of RAV1 RNA extraction buffer (Macherey-Nagel) during two rounds of 30 sec at 5,000 rpm in a TissueLyser II grinder (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids for transfections were prepared using the Nucleobond Xtra midiprep kit (Macherey-Nagel) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... Plasmid maxiprep DNA isolation was performed using NucleoBond Extra Maxi Plus EF Kit (Macherey-Nagel, Germany).
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from cell culture fluids using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Germany). Total RNA from cells was isolated using TRIreagent (Sigma ...
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bioRxiv - Microbiology 2021Quote: RNA from VTMs or swab-immersed TE buffer or cell culture supernatant was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG; 740956.250). The SARS-CoV2 RNA was quantified using (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Immunology 2021Quote: ... Barcoded amplicons were pooled and purified using Nucleo Mag NGS beads (Macherey-Nagel), then sequenced using HiSeq 2500 (Illumina) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from tissues using NucleoSpin RNA (MACHEREY-NAGEL). cDNA was synthesized using either PrimeScript reverse transcriptase (TAKARA Bio ...
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bioRxiv - Immunology 2021Quote: ... or RA1 buffer supplemented with β-mercaptoethanol (Macherey-Nagel). All samples were stored at -80°C until processing ...
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bioRxiv - Immunology 2021Quote: ... identical conditions were used except DMEM was used instead of HCM media and RNA extractions were performed using only the NucleoSpin RNA kit (Macherey-Nagel). Where ruxolitinib treatment is indicated ...
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bioRxiv - Immunology 2021Quote: Total cellular RNA from IFN-treated hAEC cultures was extracted with the NucleoMag RNA kit (Macherey-Nagel) according to the manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... apical washes of Human coronavirus 229E (HCoV-229E) and MERS-CoV-infected hAECs were extracted using the NucleoMag VET (Macherey-Nagel) according to manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... Genomic DNA was extracted using a Nucleospin tissue DNA extraction Kit (Macherey-Nagel, Hoerdt, France). Each DNA sample was eluted in 100 µl of sterile water and DNA sequencing was commissioned to Novogene facility (London ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction was performed on (i) the frozen suspension and (ii) the collected CFU suspension with the NucleoSpin® 96 Food kit (Macherey-Nagel) following the supplier’s recommendations.
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bioRxiv - Cell Biology 2021Quote: To clone the NERCLIN cDNA we extracted total RNA from 143B cells using Mini spin kit (Macherey-Nagel). Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting PCR product was purified using a standard PCR purification kit (NucleoSpin® Gel and PCR Clean-up kit, Macherey-Nagel) and used as a template for the second PCR that used the gene-specific forward primer and a 3’ T7 universal reverse primer targeting the forward linker sequence for the antisense probe ...
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bioRxiv - Genomics 2021Quote: ... radiodurans was extracted using the NucleoSpin DNA Microbial Mini kit (Macherey-Nagel). D ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNAs have been extracted on an agarose gel and purified (using the PCR clean-up gel extraction kit #740609.50, Macherey-Nagel) and the sanger sequencings have been performed by Eurofins ...
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bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
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bioRxiv - Cell Biology 2021Quote: mRNA from control and TMEM24 knockdown cells were extracted using the NucleoSpin RNAPlus kit (Macherey-Nagel). RT-PCR was performed by QuantiTect SYBR Green RT-PCR kit (Qiagen ...