Labshake search
Citations for Macherey-Nagel GmbH :
2801 - 2850 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Zoology 2020Quote: ... DNA was extracted following the standard protocol of the NucleoSpin® Tissue Kit (Macherey-Nagel) except that we performed two elution steps ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA was isolated using the Nucleospin RNA isolation kit (Macherey-Nagel)(BioDetection Systems ...
-
bioRxiv - Cell Biology 2020Quote: Total RNA isolation was performed using the NucleoSpin RNA XS Kit (Macherey-Nagel GmbH &Co.KG). Purification of poly-A containing mRNA molecules followed by mRNA fragmentation ...
-
bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR products from the first reaction were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) and used (1 µL ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids were isolated from bacteria clones with NucleoSpin Plasmid Easypure kit (Macherey-Nagel) and screened using digestion with PvuI enzyme ...
-
bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
-
bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
-
bioRxiv - Immunology 2020Quote: ... The amplified DNA was purified (Nucleospin Gel and PCR Clean-up Kit, Macherey-Nagel, Cat # 740609.250) and subsequently mutagenized by error-prone PCR (ePCR ...
-
bioRxiv - Immunology 2020Quote: ... amplicons were separated by gel electrophoresis and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Library quantification and quality control was performed using Qubit (QIAGEN ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmid DNA was purified by NucleoSpin column (Macherey-Nagel), then analysed on a 1% agarose gel containing RedSafe™ (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was quantified using the Protein Quantification Assay kit (Macherey-Nagel). The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
-
bioRxiv - Genetics 2020Quote: ... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR products were purified with a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), eluted with 50 µl elution buffer and 5 µl aliquots were analyzed with agarose loading buffer on a 2% agarose gel containing ethidium bromide
-
bioRxiv - Plant Biology 2020Quote: ... mRNA were isolated using the NucleoSpin RNA Plant kit (Macherey-Nagel) then library preparation and PE-150 bp Illumina sequencing was conducted by Novogene.
-
bioRxiv - Biochemistry 2020Quote: ... The sampling unit was pelleted on the bottom of the tube and the supernatant was transferred into HPLC vials equipped with a 200 μL V-shape glass insert (both Macherey-Nagel GmbH & Co.KG) and analysed by LC-MS/MS ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted using the Nucleospin RNA XS kit (740902.10, Macherey-Nagel). Extracted RNA was used for cDNA synthesis using the SuperScript III kit (Thermo Fisher 18080044 ...
-
bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) and ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted using the NucleoSpin miRNA kit including a DNase treatment step (Macherey-Nagel, Düren, Germany) and assessed the purity and quantity of RNA with a Nanodrop 2000 spectrophotometer (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... Individual locusts were introduced into 60 mL screw neck headspace vials (Macherey-Nagel, Dueren, Germany). The vial was sealed with TPFE 3.2 mm Beige screw cap (Marcherey-Nagel) ...
-
bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
-
bioRxiv - Genomics 2020Quote: We used the NucleoSpin® RNA (Macherey-Nagel) kit for isolation of the total RNA from the samples as was described in our previous publications [46] ...
-
bioRxiv - Immunology 2020Quote: ... Total RNA was isolated using the NucleoSpin RNA XS kit (MACHEREY-NAGEL, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... followed by column clean up with NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel). The digestion efficiency was verified by 1% agarose gel ...
-
bioRxiv - Plant Biology 2020Quote: ... fine ground plant material with the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and plasmids were purified with a Plasmid Miniprep Kit (Macherey-Nagel).
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR fragments were purified using a PCR Purification Kit (Macherey-Nagel, Duren, Germany), and plasmids were purified with a Plasmid Miniprep Kit (Macherey-Nagel).
-
bioRxiv - Genomics 2020Quote: ... Plasmids were extracted from positive clones with the NucleoSpin Plasmid kit (Macherey-Nagel) following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... tenuis females with the NucleoSpin DNA Insect Kit (Macherey-Nagel, Düren, Germany) according the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA isolation was performed using the NucleoSpin RNA XS Kit (Macherey-Nagel GmbH &Co.KG). Gene specific primers were designed by use of Primer 3 software version 0.4.0 (MIT Center for Genome Research ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA transcript was further purified with the NuceloSpin RNA Mini kit (MACHEREY-NAGEL, Germany) and quantified with spectrophotometer at 260 nm ...
-
bioRxiv - Physiology 2020Quote: ... Genomic DNA from 32 MPM cell lines was extracted with Nucleospin Blood kit (Macherey-Nagel, Düren, Germany) and 500 ng were hybridized to Affymetrix CytoScanHD Arrays (Affymetrix ...
-
bioRxiv - Molecular Biology 2020Quote: ... until glucose depletion (monitored using QuantoFix® Glucose, Macherey-Nagel, USA). After the incubation period the cells were spun down and the supernatant was filter-sterilized (PES ...
-
bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was purified by column capture (Macherey-Nagel Nucleospin mini kit) and sequenced (Eurofins Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was extracted using the Nucleospin soil kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from single legs of individual mosquitoes or whole individual mosquitoes using NucleoSpin DNA Insect Kit (Machery-Nagel) or NucleoSpin Tissue Kit (Macherey-Nagel) following the manufacturers’ instructions ...
-
bioRxiv - Microbiology 2020Quote: We extracted DNA from the biomass pellets stored at −20°C using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: The DNA was extracted using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). Then ...
-
bioRxiv - Microbiology 2020Quote: ... Genomic DNA (gDNA) was extracted from the pellets using the NucleoSpin tissue kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... primers 4163-4183 and 4288-4308) were amplified by PCR (Rotor Gene 3000, Corbett Research) from 50ng DNA (Nucleospin Tissue kit, Macherey-Nagel). PCR products quantified with Sybr green (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were supplemented with 20 mM imidazole and incubated with Protino Ni-NTA agarose beads (Macherey-Nagel) for 1 hour at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... and RNA was isolated using the NucleoSpin RNA XS kit (Macherey-Nagel, Germany) following the manufacture’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total RNA isolated earlier using the NucleoSpin RNA XS kit (Macherey-Nagel) was converted to the first-strand cDNA following the method described earlier [80] ...
-
bioRxiv - Molecular Biology 2020Quote: ... the total genomic DNA from plasmids was extracted from fresh transformed bacteria using a plasmid DNA Maxi Prep kit (Nucleobond XtraMaxi EF, Macherey-Nagel). Transgenic control GFP fluorescent marker expressing line (NB30 ...
-
bioRxiv - Zoology 2020Quote: ... urticae (one butterfly individual per nest) collected across 8 sites spread across the latitudinal gradient using the NucleoSpin® 96 Tissue kit (Macherey-Nagel) (Figure 1) ...
-
bioRxiv - Zoology 2020Quote: Total RNA was isolated from specific tissues using a kit from Macherey-Nagel (Hoerdt, France). Moloney murine leukemia virus reverse transcriptase (New England Biolabs ...
-
bioRxiv - Plant Biology 2020Quote: Leaf material of soil grown plants was collected and homogenized in liquid nitrogen prior to extraction of RNA with the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s advice ...