Labshake search
Citations for Macherey-Nagel GmbH :
2601 - 2650 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitated DNA was purified with NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was then loaded on a Protino® GST/4B column 1 ml (Macherey-Nagel) for pull-down experiments ...
-
bioRxiv - Biochemistry 2020Quote: ... Solanum tuberosum and Manihot esculenta using NucleoSpin® Food kit (MACHEREY-NAGEL GmbH & Co. KG) according to the manufacturer’s instruction.
-
bioRxiv - Biochemistry 2020Quote: ... Contaminating genomic DNA was removed using DNase and the total RNA sample was further purified with a NucleoSpin RNA Clean-up kit (Macherey-Nagel). Sequencing was then performed on an Illumina HiSeq 1500 instrument as 101 bp single read runs.
-
bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
-
bioRxiv - Biochemistry 2020Quote: ... After proteolytic cleavage the protein was incubated with 1.5 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) for 25 min at 4°C to remove His6-SUMO (for purification of SUMO-Hsc70 overnight digestion with Ulp1 and second incubation with Protino Ni-IDA was omitted) ...
-
bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
-
Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The extract was filtered and concentrated in vacuo using rotary evaporation, and the concentrated crude extract was applied to a C18 column (Polygoprep C18, 25–40 μm, 100 Å (Macherey-Nagel), 40 i.d ...
-
Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The concentrate was applied to a C18 column (Polygoprep C18, 25–40 μm, 100 Å (Macherey-Nagel), 40 i.d ...
-
bioRxiv - Biochemistry 2020Quote: ... The bound fraction was transferred to a gravity flow column (Macherey-Nagel, Cat. No. 745250.10) and washed with 15 CV washing buffer (25 mM Imidazole in PBS ...
-
bioRxiv - Immunology 2020Quote: ... and 975 µL of Buffer NPB2 (NucleoMag Pathogen kit, Macherey-Nagel). After centrifugation ...
-
bioRxiv - Immunology 2020Quote: ... were prepared with 20 µL of NucleoMag B-Beads (NucleoMag Pathogen kit, Macherey-Nagel) and 975 µL of Buffer NPB2 (NucleoMag Pathogen kit ...
-
bioRxiv - Neuroscience 2020Quote: RNA extraction was performed with NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France) in two-pooled biopsies (see Western Blot section ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extractions were performed with NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
-
bioRxiv - Neuroscience 2020Quote: ... All plasmids were purified using NucleoBond Xtra purification kit (MACHEREY-NAGEL Inc., Bethlehem, PA).
-
bioRxiv - Neuroscience 2020Quote: ... The PCR products from genotyping were purified (Macherey-Nagel) and sequenced (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: RNA was isolated from cultured cells using the NucleoSpin® RNA XS kit (Macherey-Nagel). M-MLV reverse transcriptase and random hexamers (both from Promega ...
-
bioRxiv - Cancer Biology 2020Quote: ... 50 μg/ml proteinase K (PK, Macherey-Nagel, Düren, Germany, 740506)) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Input and ChIP sample crosslinking were reversed at 65°C overnight in 50 μl TE + 200 mM NaCl + 0.1% SDS + 20-40 μg PK (Macherey-Nagel 740506). For sequencing ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was isolated from sorted E14.5 fetal liver hematopoietic progenitor cells (KL; CD45+/Lineage−/cKit+/Sca1−) using the NucleoSpin RNA Plus XS kit (Macherey-Nagel). The RNA-sequencing libraries were prepared using KAPA RNA Hyper Prep Kit with RiboErase ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated using either NucleoSpin RNA Plus purification kit (Macherey-Nagel) or ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was extracted using the RNA NucleoSpin II kit (Macherey-Nagel) and treated with DNase (Ambion Turbo DNA-free ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were lysed with Nucleozol™ (Macherey-Nagel, 740404) and RNA extraction was conducted according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA from the lysed cells was extracted with Nucleospin RNA II Kit (Macherey-Nagel). The tumors were first lysed with Trisure reagent (Bioline ...
-
bioRxiv - Cancer Biology 2020Quote: ... and RNA was further purified with the Nucleospin RNA II Kit (Macherey-Nagel). cDNA synthesis was performed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA from cell lines was extracted using the Nucleospin RNAII kit (Macherey-Nagel) and 1μg was reverse-transcribed using the iScript cDNA Synthesis kit (BioRad) ...
-
bioRxiv - Cancer Biology 2020Quote: ... All amplified PCR products were isolated using silica membrane spin column technique (NucleoSpin® Gel, PCR clean up kit Macherey-Nagel, Germany) and were eluted in 20 µl of nuclease free water ...
-
bioRxiv - Cancer Biology 2020Quote: ... NucleoSpin® Tissue kit (Macherey-Nagel, Germany) was used to process genomic DNA from 25 mg of brain tumour biopsies and from 0.5ml of PBMC samples from each patient ...
-
bioRxiv - Cancer Biology 2020Quote: ... or NucleoSpin Tissue kit (routine genotyping; Macherey-Nagel #740952.25). OneTaq DNA polymerase (New England BioLabs #M0480 ...
-
bioRxiv - Evolutionary Biology 2020Quote: Fifteen microliters of extract were injected into an Agilent 1100 series HPLC and compounds were separated on an EC 150/4.6 NUCLEODUR® C18 Gravity column (3 μm, 150 mm x 4.6 mm, Macherey-Nagel, Düren, Germany). Cardenolides and bufadienolides were eluted at a constant flow of 0.7 ml/min at 30°C with an acetonitrile–H2O gradient as follows ...
-
bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from leaves harvested at the indicated time point using the NucleoSpin® RNA Plus kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR products were purified using NucleoSpin® kit (Macherey-Nagel). Plasmids generated ...
-
bioRxiv - Biochemistry 2020Quote: ... After gel extraction of the DNA fragments with the PCR Clean-up Gel Extraction Kit (Macherey-Nagel, Düren, Germany), the DNA fragments and the vector DNA were digested by the same restriction enzymes (Supplemental Table S4 ...
-
bioRxiv - Biochemistry 2020Quote: ... Cleared extract was batch incubated with Ni-NTA beads (Macherey-Nagel) for 1 hour on a rotating wheel at room temperature for His-tag affinity purification ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was isolated using NucleoSpin® RNA from Macherey-Nagel (Düren, Germany). cDNA was generated with 500 ng RNA using High Capacity cDNA Reverse Transcription Kit from Applied Biosystems (www.appliedbiosystems.com ...
-
bioRxiv - Plant Biology 2020Quote: ... DNA recovered after ChIP and DNA from input chromatin was extracted by DNA isolation kit (Macherey-Nagel, Germany) and then analysed by qPCR ...
-
bioRxiv - Neuroscience 2020Quote: ... All plasmids used in this study were purified by NucleoBond Xtra purification kit (MACHEREY-NAGEL). pLenti-CMV-Itga6-Myc-DDK-P2A-Puro vector was purchased from Origene Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... total RNA was extracted (Macherey-Nagel) and cDNA was synthesized (Quanta Biosciences ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR products were purified by PCR clean-up/Gel extraction kit (MACHEREY-NAGEL) and cloned into pBluescript SK (+) ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR product was purified with anion exchange column AX2000 (Macherey-Nagel, Cat. No. 740525) and precipitated by IPA (IBI Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... The NucleoSpin Soil kit (Macherey-Nagel) was used to extract DNA/RNA from sediment ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted using Trizol followed by clean-up on RNeasy Plant Mini kit columns (Macherey-Nagel). 3’end librairies were prepared using the QuantSeq 3’ Fwd library prep kit (Lexogen) ...
-
bioRxiv - Microbiology 2020Quote: ... ChIP-DNA and input DNA were purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA concentration of the input DNA was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).
-
bioRxiv - Plant Biology 2020Quote: ... The fragment was purified using a NucleoSpin® kit (Macherey-Nagel, Düren, Germany) and cloned into the pENTR/SD/D-Topo vector (Invitrogen ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR products of Acc1 were column-purified with the NucleoSpin Extract ΙΙ Kit (Macherey-Nagel).
-
bioRxiv - Plant Biology 2020Quote: ... The ready-to-use NucleoSpin Plant Kit (Macherey-Nagel, Düren, Germany) was used for extraction.
-
bioRxiv - Plant Biology 2020Quote: ... we decided to stay with NUCLEOSPIN Plant II Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) which seems to be is a good trade-off between efficiency and simplicity ...
-
bioRxiv - Plant Biology 2020Quote: ... DNA was extracted using either a MO BIO PowerPlant DNA Isolation Kit (MO BIO Laboratories, Carlsbad, California, USA) or NUCLEOSPIN Plant II Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). Dry plant leaf material (typically ...