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Citations for Macherey-Nagel GmbH :
2051 - 2100 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was diluted with 4.5 ml water and subjected to solid-phase extraction on HR-XC (Chromabond, Macherey-Nagel, Düren, Germany) column ...
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bioRxiv - Cell Biology 2021Quote: ... gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel), and cloned into the LentiORF pLEX-MCS Vector (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted using the NucleoSpin Plasmid DNA Kit (Macherey-Nagel, Germany).
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bioRxiv - Microbiology 2021Quote: ... Cells were collected in Nucleoprotect RNA solution (Macherey-Nagel, Dueren, Germany). Bacteria were gated as described above ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, ref. 740609.50). Plasmids were purified using the NZYMiniprep kit (NZYTech ...
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bioRxiv - Microbiology 2021Quote: ... The supercoiled pUC19 (pUC19sc) was extracted from 100 ml overnight cultures using the NucleoBond Xtra Midi kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... purified using a NucleoMag NGS Clean-up and Size Select kit (Macherey-Nagel Inc., USA) and indexed ...
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bioRxiv - Microbiology 2021Quote: ... 30 min) to remove cell debris and the supernatant was collected and mixed with Protino Glutathione Agarose 4B (Macherey-Nagel # 745500.10) equilibrated with PBS buffer ...
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bioRxiv - Microbiology 2021Quote: ... Samples were immediately filtered using 0.45 μm regenerated cellulose membrane (Macherey-Nagel, Germany) and stored at 4 °C for maximum 2 weeks until liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Genetics 2021Quote: ... Vacuum the EconoSpin plate on the vacuum manifold (MACHEREY-NAGEL, Dueren, Germany) until no droplet drop down ...
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bioRxiv - Genetics 2021Quote: ... The lysates were then purified to genomic DNA and RNA using NucleoSpin Tissue Kits and NucleoSpin RNA Plus kits (MACHEREY-NAGEL), respectively ...
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bioRxiv - Genetics 2021Quote: ... Each tissue sample was sonicated for a few seconds with the buffer LBP1 from NucleoSpin RNA Plus kits (MACHEREY-NAGEL) and divided into two tubes ...
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bioRxiv - Genomics 2021Quote: ... samples were homogenized in Trizol reagent (Macherey-Nagel) at a ratio of 400 μL of fluid per milliliter of reagent and total RNA was extracted according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... total cellular RNA from the pretreated cells were isolated with the NucleoMag RNA kit (Macherey-Nagel) according to the manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... PCR of genomic DNA extracted 3 days after nucleofection using NucleoSpin Tissue kit (Macherey-Nagel) was performed using specific primers and Sanger sequenced (Stabvida ...
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bioRxiv - Microbiology 2021Quote: ... viral RNA was isolated with the NucleoSpin RNA Virus kit (Macherey-Nagel, Leiden, The Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Liquid samples were filtered through a 0.2 μm membrane (Chromafil® Xtra PET-20/25, Macherey-Nagel, Germany) prior to the analysis of liquid metabolites using a Shimadzu high-performance liquid chromatograph (HPLC ...
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bioRxiv - Microbiology 2021Quote: ... or cells (NucleoSpin RNA plus kit, Macherey-Nagel) at different time post-electroporation (4 hpe – 7 dpe) ...
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bioRxiv - Microbiology 2021Quote: ... reactions were cleaned up with the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel), and ligated to pGEM-T Easy (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was prepared using a NucleoSpin kit (Macherey-Nagel) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted with the Nucleospin® Tissue kit (Macherey-Nagel) from polyene-resistant and wild-type mid-log promastigotes (1 x 107 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from 10 ml of culture using the NucleoSpin RNA kit (Macherey-Nagel Inc., PA, USA) as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Microbiology 2021Quote: ... using the NucleoSpin Plasmid Mini Kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... purified using NucleoSpin RNA Clean-up (Macherey-Nagel, Hoerdt, France) and eluted in 50 μl of nuclease-free water.
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bioRxiv - Physiology 2021Quote: ... Samples were then derivatized with N-methyl-N-(trimethylsilyl)-heptafluorobutyramide (Macherey-Nagel, Düren, Germany) for 1 h at 60°C ...
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bioRxiv - Microbiology 2021Quote: ... using commercially available kits (Macherey-Nagel). Reporter plasmids pRH037 ...
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bioRxiv - Microbiology 2021Quote: ... purified (gel and PCR clean-up kit, Macherey-Nagel) and subsequently sequenced by the use of primers oRH068 ...
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bioRxiv - Microbiology 2021Quote: ... ljungdahlii with the NucleoSpin Tissue Mini kit (Macherey-Nagel) and used as PCR-template ...
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bioRxiv - Biophysics 2021Quote: ... and then incubated with Ni-IDA matrix (Protino, Macherey-Nagel) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated with Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany) for 30 min at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
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bioRxiv - Biophysics 2021Quote: ... and purified by Ni-IDA-chromatography (Protino, Macherey-Nagel) and anion-exchange chromatography (Resource™ Q ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR amplicons were purified on NucleoSpin® Gel and PCR Clean- up (Macherey-Nagel). Template DNAs of interest (500 ng each ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted using NucleoBond Xtra Midi EF kit (Macherey-Nagel). Knockout of target proteins was verified by immunoblotting.
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bioRxiv - Microbiology 2021Quote: ... the aqueous phase was transferred to a RNA extraction column and processed according to the manufacturer’s instructions (NucleoSpin RNA; Macherey-Nagel GmbH & Co, Germany). cDNA was synthesized by reverse transcription of 500ng of total RNA using either both oligo(dT)18 (0.25µg ...
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bioRxiv - Microbiology 2021Quote: ... or the NucleoSpin Blood XL kit (Macherey-Nagel), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel, Cat #740955.250) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... produced using an endotoxin-free kit (Macherey-Nagel), were co-injected with the I-SceI meganuclease (Grabher et al. ...
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bioRxiv - Microbiology 2021Quote: ... cleaned (NucleoSpin, Macherey-Nagel), and digested with I restriction enzyme ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 12,000 ×g at 4 °C for 20 minutes and the cleared supernatant was either directly used for the reconstitution assays or subjected to purification with Ni-NTA agarose (Macherey-Nagel). The suspension was incubated for 60 minutes and transferred to a fretted column ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were purified by using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel). Oligos were synthesized by TAG Copenhagen A/S and DNA sequences were sequenced at Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: DNA and RNA extractions were performed using the RNA x DNA from soil kit (740143, Macherey-Nagel, Germany). The RNA sample was treated with Turbo DNA-free Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The DNA was purified with NucleoSpin® gDNA Clean-up (Macherey-Nagel).
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bioRxiv - Microbiology 2021Quote: ... as well as cKD TgISU1 (incubated with or without ATc at 1.5 μg/mL for 3 days) were extracted using Nucleospin RNA II Kit (Macherey-Nagel). The cDNAs were synthesized with 450 ng of total RNA per RT-PCR reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... half of the cells was lysed in RA1 lysis buffer (Macherey-Nagel) (“on ice”) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 16 h after transfection and total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Microbiology 2021Quote: ... total RNA was extracted from all cell lysates using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Microbiology 2021Quote: ... PCR products were gel-purified (NucleoSpin gel and PCR clean-up kit, Macherey-Nagel) and cloned into pCR-Blunt II-TOPO vectors using the Zero Blunt TOPO PCR Cloning Kit (Thermo) ...