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Citations for Macherey-Nagel GmbH :
2251 - 2300 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA extraction was done with the NucleomagTM 96 Tissue kit (Macherey-Nagel) in combination with a Kingfisher FlexTM extraction robot (serial number 711-920 ...
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bioRxiv - Evolutionary Biology 2021Quote: We extracted both DNA and RNA from the RNAlater samples using the Nucleospin XS Kit in combination with the Nucleospin RNA/DNA Buffer Set (Macherey-Nagel), and we extracted DNA from the ethanol samples using the DNeasy Blood and Tissue kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted using NucleoSpin RNA Plus kit (#740984.50, Macherey-Nagel). Reverse transcription was performed with the High capacity cDNA Reverse Transcription kit (#4368814 ...
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bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
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bioRxiv - Genomics 2021Quote: ... by loading 400 ng of each pooled sample on an agarose gel followed by a cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Pathology 2021Quote: Amplicons were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and submitted to cycle-sequencing reaction by BigDye Terminator v.3.1 Cycle Sequencing kit (Ther-mo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified with the NucleoSpin RNA kit (Cat# 740955.50, Macherey-Nagel). For the bulk scRNA-seq samples and the cell lines ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted from each time point samples using the NucleoSpin RNA kit (Macherey-Nagel) as described in our earlier publications (Oláh et al ...
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bioRxiv - Immunology 2021Quote: Total mRNA was isolated using the NucleoSpin kit (Macherey-Nagel) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: RNA was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 either using a commercial kit (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Microbiology 2021Quote: Transformants genomic DNA was extracted with the NucleoSpin® Tissue kit (Macherey-Nagel, Düren, Germany) and further analyzed by (i ...
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bioRxiv - Microbiology 2021Quote: ... total cellular RNA was isolated using the NucleoSpin RNA extraction kit (Macherey-Nagel/Takara, San Jose, California). cDNA was generated from bulk RNA with the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... PCR products were purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and sequenced with appropriated primers (Suppl ...
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bioRxiv - Genomics 2021Quote: DNA was purified from collected cells using the Nucleospin Tissue XS kit (Macherey-Nagel) according to the manufacturer instructions and genotyped by nested PCR using primers described in Suppl ...
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bioRxiv - Genomics 2021Quote: ... BACs were extracted from bacterial culture using the NucleoBond® Xtra Midi plasmid extraction kit (Macherey-Nagel) according to the manufacturer’s provided instructions ...
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bioRxiv - Genomics 2021Quote: ... the DNA was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Genetics 2021Quote: ... BACs purified using the NucleoBond BAC kit (Macherey-Nagel) and spanning genomic regions of HuweI (RP24-157H12 ...
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bioRxiv - Genetics 2021Quote: ... 500ml of LB media with Ampicillin were inoculated with the rest of the mixture and incubated overnight for subsequent plasmid purification using the NucleoBond Xtra Maxi Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Genetics 2021Quote: ... purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries from the primary screen were sequenced 2×50bp on the HiSeq 2500 Platform (18 pM loading concentration ...
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bioRxiv - Genetics 2021Quote: ... The PCR amplicon was gel-purified by using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Mini kit (Macherey-Nagel, Düren, Germany) and verified by Sanger sequencing (Genewiz ...
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bioRxiv - Evolutionary Biology 2021Quote: ... placed in 100 μl of T1 extraction buffer (Nucleo-Spin Tissue, Macherey-Nagel), and stored at −80°C until DNA extraction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... equipped with a photodiode array detector and a semi-preparative RP-Nucleodur C18 Htec column (5 µm, 250 × 10 mm, Macherey-Nagel, Düren, Germany) using a water-acetonitrile gradient ...
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bioRxiv - Genetics 2021Quote: DNA extraction from fresh and scale connective tissues was performed using the NucleoSpin® Tissue Kit (Macherey-Nagel, Hoerdt, France), following manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: RNA was extracted using a Nucleospin RNA XS (Macherey-Nagel, Germany) kit according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and a ∼500 bp portion around the predicted CRISPR cut site was amplified by PCR and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). This fragment was then ligated into an EGFP-N vector using Xho1 and Apa1 restriction sites and transformed into DH5-a competent cells ...
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bioRxiv - Cell Biology 2021Quote: ... flanking Cas9 targeted site was amplified from genomic DNA extracted from wild type MCF10A cells (NucleoSpin tissue extraction kit, Macherey-Nagel), Puro-T2A were amplified from the custom-made plasmid MXS Puro bGHpA using primers containing T2A sequence ...
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bioRxiv - Genomics 2021Quote: ... the DNA was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Physiology 2021Quote: ... Ak1 cDNA and plasmids were digested with SpeI and BglII and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Ligation was performed using Ligation High Ver ...
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bioRxiv - Biochemistry 2021Quote: ... total RNA from HEK293 cells were isolated using the NucleoSpin RNA kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using the iScript kit (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). For each sample ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was prepared from tissues or cells using the NucleoSpin RNA II kit (Macherey-Nagel), or prepared from the isolated islets using the NucleoSpin RNA XS kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2021Quote: ... or prepared from the isolated islets using the NucleoSpin RNA XS kit (Macherey-Nagel). 1 μg of RNA was reversely transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Biochemistry 2021Quote: ... The Ni2+-NTA column (4 ml; Macherey-Nagel, Düren) was equilibrated with ten column volumes of the solubilization buffer before loading the sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Released DNA was purified (Macherey-Nagel ™ NucleoSpin ™ gel and PCR cleaning kit ...
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bioRxiv - Microbiology 2021Quote: ... After sequence verification plasmids were isolated using NucleoBond Xtra Midi plasmid DNA purification (Macherey-Nagel). For recombinant antibody expression ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting SORL1-202 amplicons were spin-column purified using a Nucleospin Gel and PCR Clean-up Kit (#740609, Macherey-Nagel) prior to validation by DNA sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using Nucleospin® 96 Tissue Kit according to the manufacturer’s protocol (Macherey-Nagel, Germany). Individual double-digest RAD-seq libraries were constructed with PstI and MseI according to the protocol detailed in Brelsford ...
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bioRxiv - Neuroscience 2021Quote: ... the probes were purified using the Nucleospin RNA clean-up kit (Macherey-Nagel, Hoerdt, France) and the concentration was measured using the Epoch Spectrophotometer System (BioTek ...
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bioRxiv - Neuroscience 2021Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissues with a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers (forward 5’ to 3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... for NucleoSpin® DNA FFPE XS (Macherey-Nagel) and QIAamp DNA Micro (Qiagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we used the Nucleospin Kit (Macherey-Nagel, USA) following manufacturer guidelines but with 60 µl instead of 100 µl of elution buffer added in the final step ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were previously purified by Midiprep using the NucleoBond Xtra Midi Kit (Macherey-Nagel, Düren, Germany) and resuspended in distilled water ...
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bioRxiv - Cell Biology 2021Quote: ... and the mRNA was purified using a NucleoSpin RNA mini kit (Macherey-Nagel), following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... PCR amplicons were purified using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel, Düren, Germany) and subjected to Sanger sequencing for identifying mutations ...
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bioRxiv - Developmental Biology 2021Quote: ... gRNA constructs were linearized by BamHI digestion and purified by column (Macherey-Nagel). gRNA was synthesized by in vitro transcription using MEGAshortscript T7 Transcription kit (ThermoFisher Scientific) ...