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Citations for Macherey-Nagel GmbH :
2101 - 2150 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from cells with the NucleoSpin RNA II kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... RNA extraction was performed using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen Carlsbad ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from frozen plant samples using the NucleoSpin RNA Plant kit (MACHEREY-NAGEL). cDNAs were synthesized using SuperScript VILO (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... coli and purified using Protino Ni-IDA Resin (Macherey-Nagel, Düren, Germany). The EMSA experiments were conducted as described previously (Wu et al ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted using the NucleoSpin 96 kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 107 cells were inoculated in 50 ml LB medium supplemented with 100 µg/ml spectinomycin and grown overnight at 37 °C for purification of the plasmid library DNA by miniprep (NucleoSpin Plasmid, Macherey-Nagel). The efficiency of fragment insertion (100% ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from tissues using a NucleoSpin RNA Virus mini kit (Macherey-Nagel, 740956) at SCVL ...
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bioRxiv - Microbiology 2021Quote: ... and column purified (Macherey-Nagel, Germany). Primers contained a 5’-overhang compatible with Illumina NGS library preparation ...
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bioRxiv - Microbiology 2021Quote: ... and 2,5μL of RNAse (Macherey-Nagel) at 1000 unit and 3,5mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted using a DNA Extraction Kit (Macherey-Nagel, #740952.50) and used for qPCR as described before [56] ...
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bioRxiv - Molecular Biology 2021Quote: ... and NucleoSpin® RNA kit (Macherey-Nagel, #740955.250) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were analyzed via thin-layer chromatography (Polygram CEL 300, Macherey-Nagel) using 0.5 M potassium phosphate (pH 3.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Genomic DNA from overnight cultures was extracted using the NucleoSpin Microbial DNA kit (Macherey-Nagel, Düren, Germany). Construction of libraries for single-nucleotide variant detection and generation of 150 bp paired-end reads on an Illumina Novaseq 6000 platform ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We purified PCR products with a NucleoFast 96 PCR kit (Macherey-Nagel, USA) or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare ...
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bioRxiv - Zoology 2021Quote: ... standard DNA extraction was performed using the NucleoSpin™ Plant II DNA extraction kit (Macherey-Nagel GmbH and Co., Düren NRW, Germany). A reasonable number (usually 30–70 ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from U2OS and HeLa cell pellets using a Nucleospin RNA isolation kit (Macherey-Nagel) according to the manufacturer’s instructions and the RNA concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from liver samples using the NucleoSpin RNA Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The amplified rDNA gene fragments were purified using nucleobond AX 100 columns for Miniprep System (Macherey-Nagel, ref 740521.100). The rDNA sequences were then labeled with Digoxigenin-11-dUTP by nick translation according to the manufacturer’s protocol (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... organoids were harvested and RNA was extracted using the NucleoSpin® RNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated using the nucleospin RNA kit (Macherey-Nagel) and reverse-transcribed to cDNA (kit ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were purified using Plasmid DNA Mini Kit I (Omega Biotech, cat# D6943-02) or Nucleobond Xtra Mid kit (Macherey-Nagel, cat# 740410.50), according to protocols supplied by the manufacturers ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was isolated using the NucleoSpin RNA XS kit (Macherey-Nagel). RT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel Cat#: 740955.250). The cDNA was synthesized by RT-PCR from 1 μg of purified RNA with the M-MLV reverse transcriptase (Thermo Fisher Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... BAC DNA amplified in DH10b is extracted (Nucleobond MIDI XTRA, Macherey-Nagel), checked by Acc65I-NotI complex restriction profile ...
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in 10mM Tris-HCL pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in injection buffer (10mM Tris-HCL pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... extraction protocol or with the NucleoSpin RNA extraction kit (Macherey-Nagel, Filterservices) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA were extracted from HUVEC using the NucleoSpin RNA II kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from MEFs following treatment with 4µM thapsiragin over 24hrs with the NucleoSpin RNA plus from MACHEREY-NAGEL, following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... followed by purification using NucleoSpin RNA Clean-Up kit (Macherey-Nagel). The cDNA was synthesized from 0.5 and 1 μg of total RNA for cells and tissues ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA was collected using NucleoSpin kits (Macherey-Nagel, #740955). First-strand synthesis was carried out with the SuperScript III system (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from cultured cells using Mini spin kit (Macherey-Nagel). RNA extracted from canine fibroblasts was obtained from Prof ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stimulated with 15% FBS for 45 min and total RNA was extracted using Nucleospin RNA II kit according to the manufacturer’s protocol (Macherey-Nagel). Five hundred micrograms of total RNA wasused for complementary DNA synthesis using Thermo Scientific RT–PCR kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... We extracted the plasmids using the Plasmid Maxi prep kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2021Quote: ... Cells were either fixed with 4% paraformaldehyde or lysed with NucleoZOL (MACHEREY-NAGEL) 17 days post infection ...
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bioRxiv - Cell Biology 2021Quote: Total DNA was extracted using NucleoSpin Tissue (MACHEREY-NAGEL, Düren, Germany) from the mouse hearts or the cells cultured in 6-well plate following manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: mRNA from encysting and non-encysting KO strains was isolated by NucleoTrap mRNA Mini Column kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Total RNA Isolation was carried out with the NucleoSpin RNA Kit from Macherey-Nagel. All steps were performed according to the manufactures description ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments and plasmids were respectively purified with NucleoSpin® Gel and PCR cleanup (Macherey-nagel # 740609) and NucleoSpin® Plasmid (Macherey-Nagel #740499) ...
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bioRxiv - Cell Biology 2021Quote: ... and NucleoSpin® Plasmid (Macherey-Nagel #740499). All the entry vectors were sequenced ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µL of extracts were injected into a Nucleoshell RP 18 column (2.7 µm x 150 mm x 2 mm, Macherey-Nagel GmbH, Düren, Germany). A solvent system composed of A ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from root tissues of DKC-9144 and PG-2475 at three time points (15, 25 and 35 DAS) with or without PBZ using Nucleospin RNA plant kit (Pal et al., 2017) (Macherey-Nagel, Germany). cDNA synthesis and qPCR was performed as described previously (Pal et al. ...
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bioRxiv - Microbiology 2021Quote: ... the PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Purified PCR products were cloned into pGEM®-T Easy Vector (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The total RNA was extracted using the NucleoSpin RNA Mini kit (Macherey-Nagel, Germany) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... and then purified by column (Macherey-Nagel) as template ...
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bioRxiv - Immunology 2021Quote: RNA of naïve and polyclonally activated CD8+ T cells was purified using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol and submitted to the Scientific Genomics Platform (Max Delbrück Center for Molecular Medicine ...