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Citations for Macherey-Nagel GmbH :
2201 - 2250 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... oneistus was extracted using the NucleoSpin RNA XS Kit (Macherey-Nagel, Düren, Germany). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated using Nucleospin RNA (Macherey-nagel, Hoerdt, France) and transcribed into cDNA by Maxima First Strand cDNA synthesis Kit (Thermo scientific) ...
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bioRxiv - Microbiology 2021Quote: ... amplified DNA was purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted using either the commercial DNA extraction kits NucleoSpin Tissue (Macherey-Nagel) or DNeasy Blood & Tissue (Qiagen ...
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bioRxiv - Pathology 2021Quote: RNA was isolated from liver tissue using the NucleoSpin® RNA Plus Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Viral biodistribution analysis - Genomic DNA (gDNA) was extracted from the brain region of interest with the NucleoSpin®Tissue Kit (Macherey-Nagel) extraction kit and 100 ng of gDNA was used as a qPCR template ...
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bioRxiv - Microbiology 2021Quote: ... while RNA extraction was performed using the NucleoSpin® RNA XS Kit (MACHEREY-NAGEL) according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2021Quote: ... The NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL, Germany) were used to purify the PCR products ...
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bioRxiv - Microbiology 2021Quote: ... RNA isolation was further performed by recommended protocols (MACHEREY-NAGEL, Germany). cDNA conversion was performed from each sample by using ReverTra Ace® qPCR RT Master Mix with gDNA remover (Toyobo ...
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bioRxiv - Microbiology 2021Quote: RNA extraction was performed using Nucleospin® RNA kit (MACHEREY-NAGEL, Germany) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Amplified DNA was purified using Gel and PCR Clean-up kits (Macherey-Nagel) and single-end Sanger-sequenced with the corresponding forward primer at Eurofins Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Total RNAs were extracted from cells by using the NucleoSpin RNA isolation kit (MACHEREY-NAGEL). Eluted RNA samples were reverse transcribed by using the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: DNA was extracted using the NucleoSpin Plant II kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and following their protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... were prepared by NucleoSpin RNA kit (Macherey-Nagel). Then rRNA depletion was performed with the RiboMinus Eukaryote System v2 (Invitrogen–Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were isolated from the siRNA-treated cells using a NucleoSpin RNA kit (Macherey-Nagel). To check depletion of the siRNA-targeted proteins ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated with NucleoSpin RNA II Kit (Macherey-Nagel) according to the manufacturer’s protocol (MN ...
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bioRxiv - Systems Biology 2021Quote: ... RNA-isolation (Macherey-Nagel NucleoSpin RNA plus), reverse transcription (TaKaRa Bio PrimeScript RT with gDNA eraser ...
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bioRxiv - Synthetic Biology 2021Quote: Gel-purify the excised band using the Macherey-Nagel NucleoSpin Gel & PCR Clean-up kit (Macherey-Nagel, Germany). Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2021Quote: ... the vector pEHLYA5 in the band corresponding to 3612 bp was cleaned with Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Plasmids pCANTAB6_H107Y ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR products were cleaned with the kit NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel) and sent to Macrogen for sequencing with the oligonucleotide CDRI_Fwd ...
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bioRxiv - Synthetic Biology 2021Quote: ... MACS4EPEC and MACS5EPEC was used as template for a PCR with the primers tirDSF and tirDSR and the resulting amplicons were cleaned NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel) and sent for PacBio sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated using the NucleoSpin RNA plant isolation kit (Macherey-Nagel 740949) with on-column DNA digestion and adding Plant RNA isolation Aid (Life technologies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was extracted from GYP pre-treated BRIN-BD11 cells using NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA was prepared with a NucleoSpin™ Plasmid EasyPure kit (Macherey-Nagel). E ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Physiology 2021Quote: ... and NucleoSpin gel purification (Macherey-Nagel, Duren, Germany). The first round of RT-PCR used primers designed against untranslated regions of a predicted Acropora digitifera Rh mRNA (XP_015769291.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was extracted using the Nucleospin Tissue kit (Macherey-Nagel) and tested by PCR to screen for genomic editing events ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA of each clonal population was extracted (Macherey-Nagel) and the level of total Neat1 and Neat1_2 expression was monitored by qPCR using specific primers (Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was loaded onto a 5 mL Ni-NTA column (Macherey-Nagel). After washing with a buffer consisting of 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
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The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cellsbioRxiv - Molecular Biology 2021Quote: ... RNA was isolated by using the NucleoSpin II RNA mini kit (Macherey-Nagel) and subjected to RNA-Seq ...
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bioRxiv - Molecular Biology 2021Quote: ... californicus frozen in liquid nitrogen with NucleoSpin RNA Plus (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragments were then purified with a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The integrity of the purified DNA fragments was confirmed by 2% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were extracted from yeast cells disrupted by bead beating and purified using the Nucleospin RNA II kit (Macherey-Nagel). For RNA-seq analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted using NucleoSpin RNA Plus (Macherey-Nagel), and single-stranded cDNA was synthesized by reverse transcription of total RNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Gel-purified amplicons were then assembled using NEBuilder HiFi DNA Assembly Master Mix at a 1:1 molar ratio for each DNA fragment and incubated at 50°C for two hours ...
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bioRxiv - Microbiology 2021Quote: ... and then column purified (Macherey-Nagel). The N expression plasmid was linearized using EcoRV-HF (NEB) ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from the cells using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The intracellular and extracellular RNA from cells was isolated using respective kits (MACHEREY-NAGEL GmbH & Co. KG) and the SARS-CoV-2 RNA was quantified using a commercial kit (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Microbiology 2021Quote: The RNA was isolated using Nucleospin Viral RNA kit (MACHEREY-NAGEL GmbH & Co. KG) and the SARS-CoV-2 RNA was quantified using a commercial kit (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Microbiology 2021Quote: RNA extraction was performed for each sample using the NucleoSpin RNA kit (Macherey-Nagel). Total RNAs were quantified using Nanodrop (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: The qPCR product for hsv1-miR-H27 was first subjected to agarose gel electrophoresis and the band complementary to the size of cDNA was excised and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Germany). The purified product was cloned into T&A™ cloning vector by using T&A™ Cloning Vector (Yeastern Biotech Co. ...
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bioRxiv - Genomics 2021Quote: ... Plasmid DNA was amplified overnight and isolated using the NucleoBond Xtra Midi Plus Kit (Macherey-Nagel). The plasmid library was sequenced paired-end 50 bp on the HiSeq 2500 platform yielding approximately 1.1*107 fragments (Supplementary Table 1) ...
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bioRxiv - Bioengineering 2021Quote: ... or NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). gRNA targeting BFP (gBFP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bacterial DNA extraction was done after 48h of fermentation using Nucleospin DNA Stool Genomic DNA Purification Kit (Macherey-Nagel, Germany) and relative abundance of selected gut bacteria was determined by qRT-PCR.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using Nucleospin DNA Stool Genomic DNA Purification Kit (Cat. No. 740472; Macherey-Nagel, Germany), as per manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NuleoSpin® RNA/Protein extraction kit (740933.50) was purchased from Macherey-Nagel (Hoerdt, France). Sequencing grade modified trypsin (V5111) ...
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bioRxiv - Biochemistry 2021Quote: ... and filtered through 0.45 μm PTFE filters prior to HPLC injection (Macherey-Nagel GmbH & Co., cat. #: 729015), with each assay performed at least twice ...