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Citations for Macherey-Nagel GmbH :
2151 - 2200 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from three independent experiments of the mock- and KSHV-infected lymphatic ECFCs isolated as an adherent subpopulation from donor 1 using the NucleoSpin RNA extraction kit (Macherey-Nagel), after which purity and concentration was determined with NanoDrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from mock- and KSHV-infected blood or lymphatic ECFCs using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). RNA was further concentrated and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified with the Gel and PCR cleanup kit (Macherey-Nagel, Dueren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... mounted on a vacuum device (NucleoVac 96, Macherey-Nagel). Alternatively ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cat#M0491S/L) and gel extractions were done with the Macherey-Nagel NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Cat#740609). DNA assemblies were carried out by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 x 109 cells were used for extraction of genomic DNA using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). PCR-free libraries (microbial short insert libraries ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from leaves using NucleoSpin RNA Plant (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted desulfoglucosinolates were separated on a reversed phase C-18 column (Nucleodur Sphinx RP, 250 x 4.6 mm, 5µm, Macherey-Nagel, Düren, Germany) with a water (A)-acetonitrile (B ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel), cDNA was synthesized using the PrimeScript RT Reagent Kit (Perfect Real Time ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from the cells with a PGTX solution42 and purified using NucleoSpin RNA (MACHEREY-NAGEL). cDNA was synthesized from 1 μg of total RNA using ReverTra Ace qPCR RT Master Mix (TOYOBO) ...
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bioRxiv - Plant Biology 2022Quote: ... NucleoSpin RNA Plant (MACHEREY-NAGEL) was used for total RNA extraction from roots or shoots ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted using Nucleospin Plant RNA kit (#740949.250 Macherey-Nagel). RNA was used to generate Lexogen Quantseq 3’ RNA seq libraries at Cornell University Institute of Biotechnology Genomics Facility ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... The culture medium was centrifuged at 6000 rpm for 20 min and then vacuum-filtrated through a filter paper (MN 615, Macherey-Nagel) and subjected to a two-step cation exchange chromatography protocol using an ÄKTA Explorer FPLC system (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... purified with the NucleoSpin RNA Clean-up XS (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and then used as the template for reverse transcription with the ReverTra Ace qPCR RT kit (Toyobo ...
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bioRxiv - Plant Biology 2022Quote: ... and sealed with an N 9 PP screw cap with a center hole and silicone white/PTFE red septum (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Plant Biology 2022Quote: ... purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and circularized using T4 DNA ligase.
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of homogenate was set aside as total cell (T) and mixed with NucleoZOL (Macherey-Nagel, #740404.200) before storage at -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... the pellet was resuspended in 500 µL of NucleoZOL (Macherey-Nagel, #740404.200) before storage at - 80°C.
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted according to NucleoZOL (Macherey-Nagel, #740404.200) manufacturer instructions and immediately processed as follows ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was amplified by PCR methods using the following oligo-DNA primers and the products were purified with micro spin columns (MACHEREY-NAGEL, 74060910). Each reverse primer also possesses T3 sequence for transcription.
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bioRxiv - Plant Biology 2022Quote: ... with the difference that a Nucleodur 100-5 NH2 125x4 mm column (Macherey-Nagel, #760730.40) and an eluent consisting of 8.5% acetonitrile and 25mM formic acid pH 4 were used (18) ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted using the RNA NucleoSpin Plant kit (Macherey-Nagel) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of 5 days old seedlings (both controls and treated with NaCl) using the NucleoSpin RNA Plus (Macherey-Nagel). Reverse-transcription was performed using using the SuperScript III First-Strand VILO cDNA Synthesis Kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... a NucleoSpin Plant II kit (Macherey-Nagel) for the Seven-parent-MAGIC and NORFAB panels ...
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bioRxiv - Genomics 2022Quote: ... After 20 hours at 30 ºC bacteria were harvested and plasmid preparations were performed using Macherey-Nagel™ NucleoBond™ Xtra Midi EF (Macherey-Nagel #740420.50).
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and cloned into the plasmid pCM009 (Schell et al. ...
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bioRxiv - Microbiology 2022Quote: ... Cloning was performed according to standard protocols and plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel). DNA fragments were amplified using Phusion High Fidelity DNA polymerase (NEB ...
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bioRxiv - Microbiology 2022Quote: ... coli clones using a Miniprep kit (Macherey-Nagel, 740588) and verified by single pass primer extension sequencing (ACGT Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... The cssDNA released from the phage were then extracted with NucleoBond Xtra Midi EF kit (Macherey-Nagel) following the manufacturer’ s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 20 µL of proteinase K (20 mg/mL; MACHEREY-NAGEL # 740506) was added and the mixture incubated at 70°C for 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
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bioRxiv - Microbiology 2022Quote: DNA extraction was performed as described in [21] using the Nucleospin tissue kit (Macherey-Nagel). DNA concentration and purity were checked with a NanoDrop One (Thermo Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: Cultured cells were lysed in Macherey-Nagel RA1 buffer containing 1% β-mercaptoethanol and total RNAs were isolated using NucleoSpin RNA isolation kit (Macherey-Nagel 740955). Reverse transcription and PCR amplification were performed as described before21 ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using a NucleoSpin Gel and PCR purification kit (Macherey-Nagel) and indexed using a TruSeq Nano DNA Library Prep kit (Illumina ...
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bioRxiv - Biophysics 2022Quote: ... Ligated DNA constructs were separated with agarose gels from other reaction products and purified by gel extraction (NucleoSpin, Macherey-Nagel) by avoiding any exposure to ethidium bromide or UV light.
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bioRxiv - Genomics 2022Quote: ... Maxiprep was performed with the Nucleobond Xtra endotoxin-free maxiprep kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from cell pellets using the RNA extraction kit (Macherey-Nagel) and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The library stock was prepared by scraping plates followed by purification using the Midi Prep kit (Macherey-Nagel). Library sequencing was performed on a MiSeq run to ensure sgRNA uniformity ...
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bioRxiv - Cancer Biology 2022Quote: ... and excised from a 0.8% agarose gel for purification using the NucleoSpin Gel Cleanup Kit (Macherey-Nagel). The insert pool and digested backbone were then ligated overnight at 16°C using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2022Quote: RNA was extracted from retinal samples using the NucleoSpin® RNA kit (Macherey-Nagel) using the manufacturer’s protocol ...