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Citations for Macherey-Nagel GmbH :
1251 - 1300 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Plant Biology 2023Quote: The RNA extraction and conversion to cDNA of tomato leaves was carried out using columnkit based on silica membranes (Macherey-Nagel XXX GmbH, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted with the NucleoSpin II kit (Macherey-Nagel) and reverse-transcribed using the High- Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... DNA and RNA were extracted from the powder using NucleoSpin TriPrep mini kit columns (Macherey-Nagel), according to the manufacturer’s main instructions but with a few modifications ...
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bioRxiv - Systems Biology 2023Quote: Total RNA was isolated using NucleoSpin RNA Plus (Macherey-Nagel, Duren, Germany, Cat. No. 740984.250). Library construction and RNALseq were performed using the Illumina sequencing platform (Rhelixa) ...
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bioRxiv - Systems Biology 2023Quote: ... equipped with a 30-m OPTIMA® 1301-MS column (6% cyanopropylphenyl, 94% dimethylpolysiloxane, 0.25 mm internal diameter, 0.25 µm film thickness; Macherey-Nagel, Düren, Germany). Injections of 1 µL aliquots of sample solution were performed in splitless or split mode depending on the peak intensities (n = 2 ...
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bioRxiv - Microbiology 2023Quote: ... and purified with the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel). The DNA binding reactions were performed in interaction buffer (10 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Immunology 2023Quote: Total RNA from cell lysates was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... or NucleoSpin Plasmid kit (Macherey-Nagel). The correct assembly of all plasmids was verified by Sanger sequencing (Microsynth Seqlab).
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bioRxiv - Synthetic Biology 2023Quote: ... or the Nucleospin gel and PCR clean-up kit (Macherey-Nagel). Restriction and ligation reactions were performed using enzymes and buffers obtained from New England Biolabs and Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Plant Biology 2023Quote: ... performed with the NucleoSpin Plasmid kit (Macherey-Nagel, Germany); the successful cloning was verified by Sanger sequencing.
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bioRxiv - Genetics 2023Quote: ... DNA was extracted using the NucleoSpin 96 Plant II Kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNAs from whole lungs or BM of naive or IAV-infected mice were extracted and purified using the NucleoSpin RNA Extraction Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated using an extraction kit according to the manufacturer’s instructions (Macherey-Nagel). The RNA concentration was assayed with a NanoDrop (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were propagated in cultures of terrific broth medium with appropriate antibiotics overnight at 37 °C and purified with NucleoSpin miniprep kit (Macherey-Nagel) following the manufacturer’s prototol ...
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bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Correct products were isolated with gel electrophoresis and gel extraction (Macherey-Nagel, F40609.250), and were ligated with T4 DNA ligase (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing was carried out on an Illumina HiSeq XTen at Macrogen ...
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bioRxiv - Microbiology 2023Quote: ... and DNA bands of interest were isolated using the NucleoSpin® Gel & PCR Cleanup Kit from Macherey-Nagel. The extracted DNA underwent Sanger sequencing at Eurofins Scientific ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA of tick organs and eggs was extracted using the NucleoSpin Tissue kit according to the manufacturer’s instructions (Macherey-Nagel, Germany). DNA was eluted with 50 μL of elution buffer and was stored at -20°C until use.
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted using either a NucleoSpin Tissue Kit (Macherey-Nagel) or the phenol-chloroform protocol [50] ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and lysed with RNA extraction buffer (Nucleo spin RNA extraction kit, Macherey-Nagel, Cat# 740955). Total RNA was extracted according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... PCR samples were then purified using following the manufacturer’s instructions (NucleoSpin® PCR and gel kit, Macherey-Nagel). Purified amplicons were verified by electrophoresis on a 1% agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA and mRNA were extracted using NucleoSpin Tissue and NucleoSpin RNA kits (Macherey-Nagel), respectively.
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50mg of root using the NucleoSpin® RNA Kit (Macherey-Nagel). cDNAs were synthesized with the M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid purification was performed using NucleoBond Xtra Midi EF Kit from Macherey-Nagel. Inserts were sequenced at Eurofins MWG Operon ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted from purified astrocyte preparations using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250). RNA was analyzed using TapeStation ...
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bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
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bioRxiv - Neuroscience 2022Quote: ... purified (Macherey-Nagel #740416.10), and packaged into AAV8 vector (packaging was done by University of Helsinki AAV core unit) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthetized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthesized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA extraction was carried out using the nucleospin RNA kit (Macherey-Nagel). The RNAs were quantified using a Nanodrop and the retro-transcription made using the high-capacity cDNA reverse transcription kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... extracellular viral RNA was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and intracellular viral RNA using the E.Z.N.A Total RNA Kit I (Omega Bio-tek) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA in each sample was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and quantified by qRT-PCR.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse cross-linked chromatin was purified using the NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were isolated using an endotoxin-free plasmid midiprep kit (Macherey-Nagel) for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50 ...
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bioRxiv - Cell Biology 2022Quote: DNA from electroporated HSC was extracted using the tissue XS kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The resulting PCR product was ‘cleaned up’ using a NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Capped and Poly(A ...
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bioRxiv - Biochemistry 2022Quote: ... Coli using NuceloBond Xtra Maxi Kit per the manufacturers recommendations (Macherey-Nagel). All scFv-ACE2 ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Bioengineering 2022Quote: ... Plasmid extraction and polymerase chain reaction (PCR) purification kits were ordered from Macherey-Nagel GmbH & Co ...
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bioRxiv - Bioengineering 2022Quote: ... purified using PCR clean-up kit (Macherey-Nagel), and transformed in electrocompetent E ...