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Citations for Macherey-Nagel GmbH :
1151 - 1200 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Supernatants collected were processed for RNA preparation using Nucleospin Viral RNA isolation kit (Macherey-Nagel GmbH & Co. KG), and infectious titer assay (plaque formation assay (PFA)) ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from cell culture supernatants using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted using a NucleoSpin Soil DNA extraction kit (Macherey-Nagel GmbH, Düren, Germany).
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitated DNA samples were purified using PCR purification column (MACHEREY-NAGEL Inc.) and eluted in 80 μL double-deionized water ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were batch-purified using Ni-NTA agarose resin (Macherey-Nagel) and eluted using the abovementioned lysis buffer with increased imidazole concentration (250 mM) ...
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bioRxiv - Pathology 2022Quote: We extracted total RNA from 10 mg liver tissue using NucleoZOL reagent (Macherey-Nagel GmbH & Co. KG, Germany). First ...
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bioRxiv - Microbiology 2022Quote: ... then in the same column the RNA was concentrated to 15 µL using the MN NucleoSpin RNA Clean-up XS (Macherey-Nagel). The same process was conducted on 2 x 200 µL of sterile water to create two blank control libraries ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated with the Nucleospin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Isolation of total RNA was performed using NucleoSpin RNA isolation kit (NucleoSpin, MACHEREY-NAGEL). cDNA was synthesized from total RNA by SuperScript® IV Reverse Transcriptase (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... excess egg water was removed and 350 μl lysis buffer added (NucleoSpin, MACHEREY-NAGEL). The larvae were homogenized by repeatedly drawing through a 26-gauge needle into a syringe and expelled ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted using the NucleoSpin Blood kit (Macherey-Nagel). The sgRNA expression cassettes were amplified from gDNA in a two-step nested PCR using Q5 High-Fidelity 2X Master Mix (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... faeces and buccal swabs were homogenized in a bead mill (Precellys) in lysis buffer (Macherey-Nagel; cat no. 740984.10). After homogenization ...
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bioRxiv - Microbiology 2022Quote: ... and total RNA was extracted by using NucleoSpin Plus RNA virus Kit (Macherey-Nagel, cat no. 740984.10). For serum (50 μl) ...
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bioRxiv - Microbiology 2022Quote: ... NucleoSpin RNA virus kit (Macherey-Nagel; cat no. 740956.250) was used for RNA extraction.
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using the Nucleospin Gel and PCR Clean up kit (Macherey-Nagel) by following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA from hiPSC-derived cells were isolated using NucleoSpin Plus RNA kit (Macherey-Nagel). A total of 1.5 μg RNA was reversely transcribed using Moloney murine leukemia virus reverse transcriptase (M1701 ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from Ana2014 cells using NucleoSpin® RNA plus (Macherey-Nagel, Germany) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified using PCR purification kit (Macherey-Nagel) and digested with NdeI and BamHI restriction enzymes ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA isolation was performed using NucleoSpin RNA kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas) according to manufacturer guidelines ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments were purified with the Gel and PCR cleanup kit (Cat. n° 740609.250, Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed with Nucleozol (Macherey-Nagel, 740404) and RNA extractions were conducted according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were purified using Macherey-Nagel Nucleospin® RNA Clean-up kit (Macherey-Nagel, Bethlehem, PA) prior to injections.
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted by using the Macherey-Nagel Nucleospin® RNA Clean-up XS kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: The genomic DNA of Rhodococcus strain IGTS8 was isolated using the NucleoSpin Tissue DNA Extraction kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid preparation and DNA gel extraction were performed using the Nucleospin Plasmid kit and the Nucleospin Extract II kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). DNA sequences were determined by Eurofins-Genomics (Vienna ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from the transformants using NucleoSpin plasmid purification kit (Macherey-Nagel). Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa) ...
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bioRxiv - Microbiology 2022Quote: ... All V3-V4 amplicons were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) and pooled in equimolar ratios ...
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bioRxiv - Microbiology 2022Quote: ... The anolyte samples were filtered using 0.2 μm membrane filters (CHROMAFIL® Xtra, Macherey-Nagel, Germany) and stored at -20°C for further analysis ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was extracted by phenol-chloroform and cleaned up using the NucleoSpin® RNA XS extraction kit (Macherey-Nagel, Germany). Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR products were isolated using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Standard molecular cloning techniques were further employed to insert the genes of interest into target vectors using restriction nucleases (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The final library was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Microbiology 2022Quote: ... overlap PCR products were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to the manufacturer’s instructions and DNA concentration was quantified by using NanoDrop spectrophotometer (A 260 nm).
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted using a Nucleospin RNA micro kit (Macherey-Nagel). cDNA synthesis and adapter ligation were performed with the SMARTer cDNA synthesis kit (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: ... and purified by NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL).
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bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted using NucleoSpin® RNA mini kit (Macherey-Nagel, Germany) including a 15 min DNAse-treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... Mini- and MidiPreps (Macherey-Nagel) were performed according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: For bacterial DNA extraction 700µL of SL1 lysis buffer (NucleoSpin Soil kit, Macherey-Nagel) was added to the stool samples and tubes were heated at 95°C for 2h to inactivate SARS-CoV-2 ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted with NuceloSpin RNA Plant and Fungi kit (Macherey-Nagel). cDNA was synthesized using RevertAid RT reverse transcription kit (Thermo Fisher) ...
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bioRxiv - Genetics 2022Quote: ... and total RNA was isolated using NucleoSpin RNA (Macherey-Nagel 740955.50, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Endotoxin free DNA was prepared using respective kits (Macherey-Nagel GmbH & Co. KG, Düren, Germany), quantified with a NanoDrop Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were cloned into the DH5a strain (Enzynomics) and purified with a NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... Dissected tissue was immediately placed into lysis buffer (Macherey-Nagel) on dry ice and stored at -80C ...
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bioRxiv - Cell Biology 2022Quote: ... ssDNA was column-purified using Buffer NTC (Macherey-Nagel) as a binding buffer ...
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bioRxiv - Cancer Biology 2022Quote: Total DNA extraction was performed using NucleoSpin® Tissue mini kit (Macherey-Nagel, Germany). RNA was extracted using NucleoSpin® RNA mini kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid DNA was isolated using the NucleoSpin Plasmid kit (Macherey-Nagel) and analysed by Sanger sequencing (Eurofins Genomics).
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Nucleo Spin RNA Blood (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and purified directly from the PCR mix or from an agarose gel (Gel and PCR clean-up kit, Macherey-Nagel); the two fragments were fused together thanks to a primer-encoded 20-30bps overlap during a second PCR amplification round and the product cloned into the pEMG suicide vector [20] using EcoRI and BamHI ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was isolated from extracts obtained from different fractions using NucleoSpin TriPrep Kit (Macherey-Nagel) following the requirements of the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial genomic DNA was extracted from faeces by using NucleoSpin 96 Soil kits (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...