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Citations for Macherey-Nagel GmbH :
1101 - 1150 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted with the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, DE) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified with NucleoSpin RNA kit (740955; Macherey-Nagel).
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bioRxiv - Cancer Biology 2023Quote: Total RNA is isolated using NucleoSpin RNAII kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted genomic DNA from faeces using the NucleoSpin®Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted DNA was eluted in NE buffer (Macherey-Nagel) and quantified with dsDNA HS assay kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... before RNA was purified using the NucleoSpin miRNA Mini kit for miRNA and RNA purification (Macherey-Nagel) without size fractionation ...
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Cell Biology 2023Quote: ... Selected colonies were cultured up and DNA was isolated from them (Macherey-Nagel, 740420.50). Successful creation of SNAP-Cldn4ECS2-mut was verified with sequencing (LGC Genomics GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... pX458 plasmid was digested with BpiI (BbsI; Thermo, #ER1011) and gel-purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel); gRNA oligonucleotides were ligated into pX458 plasmid with T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), and cloned into pGEM-T Easy Vector (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from the resulting pellets using the NucleoMag DNA Kit (Macherey-Nagel, Allentown, PA, USA) with the aid of the AutoPure96 robot for the purification step ...
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bioRxiv - Microbiology 2023Quote: mRNA of total colonic tissue or small intestinal organoids was isolated using the NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Pull-down experiments using GST (Glutathion-S-Transferase)-fused proteins were performed using glutathione agarose beads (Macherey-Nagel, Düren, Germany). The beads were incubated with the GST-fused proteins for 1 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplified samples were purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and quantified on the NanoDrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified via gel purification or column purification using the NucleoSpin Gel and PCR Clean–up kit (Macherey-Nagel, Cat.#740609.50). Amplicon sequences were verified by Sanger sequencing using the Overnight Mix2Seq Kit service (Eurofins Genomics) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cultures using the NucleoSpin Plasmid kit (Macherey-Nagel, Cat.#740588.50). All plasmids contained ampicillin resistance (ampR ...
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bioRxiv - Cell Biology 2023Quote: ... medium-scale plasmid preparations were produced using the NucleoBond Xtra Midi Kit (Macherey-Nagel).
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were purified using NucleoSpin columns (Macherey-Nagel) and eluted into 33 μL water ...
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bioRxiv - Synthetic Biology 2023Quote: ... and transferred into HPLC vials (Flat N9 vials, screw neck, 1.5 mL, clear, Macherey-Nagel, Germany) and stored at 4 ℃ until HPLC analysis ...
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bioRxiv - Synthetic Biology 2023Quote: ... for the ON-state selection was prepared by dissolving appropriate amounts of kanamycin in deionised water and filter-sterilising it through a 0.2 µm cellulose acetate filter (MN Steriliser CA, Macherey-Nagel GmbH & Co. KG, Düren, Germany). The L-arabinose (Tokyo Chemical Industry Co. ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR fragments were gel-purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL) by following manufacturer’s protocol ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Pathology 2023Quote: ... total RNA preparation was performed using the NucleoSpin RNA Plus kit (MACHEREY-NAGEL). cDNA was synthesized using random hexamer and SuperScript III (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... coli bacterial cultures using NucleoSpin Plasmid QuickPure or NucleoBond Xtra MidiPrep kits (Macherey-Nagel, Düren, Germany) according to manufactureŕs instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted with a NucleoSpin (Macherey-Nagel) RNA extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cell pellets using NucleoSpin RNA kit (Macherey-Nagel). First-Strand cDNA synthesis was performed using the SuperScript III Reverse Transcriptase kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was prepared with the NucleoSpin Microbial DNA Kit (Macherey-Nagel) and treated with 1 µL of RNase Cocktail™ Enzyme Mix (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of Glutathione Sepharose 4B (Macherey-Nagel) (75% ...
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bioRxiv - Molecular Biology 2023Quote: ... and column-purified using NucleoSpin RNA kit (Macherey-Nagel). 500ng of total RNA was used for reverse transcription using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs) ...
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bioRxiv - Plant Biology 2023Quote: ... Established plasmids were extracted by NucleoBond Xtra Maxi (Macherey-Nagel, Düren, Germany). Barley cultivar GP was employed as a recipient of transgenes because barley epidermis could be peeled off more easily than wheat epidermis for releasing protoplasts ...
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bioRxiv - Plant Biology 2023Quote: ... then added 1.2 µL of RNase (MN, Macherey-Nagel, Ref. 740505, 1200 U/mL, 10 mg/mL), and transferred the DNA to a 96-well KingFisher Flex plate (ThermoFischer Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... coli was done using NucleoSpin Plasmid (MACHEREY-NAGEL). The mutations were verified by sequencing (Microsynth ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA from CD11B+ cells was extracted using an RNA XS Plus extraction kit and that from brains using a NucleoSpin RNA extraction kit according to the manufacturers’ protocol (Macherey-Nagel®). Reverse transcription was performed using 350 ng (CD11B+ ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was isolated from cells using the NucleoSpin Tissue kit (Macherey-Nagel). 500 ng of genomic DNA was subject to bisulfite convertion using the EZ DNA Methylation-Gold kit (Zymo) ...
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA was purified from frozen hippocampi using NucleoSpin Tissue columns (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Immunology 2023Quote: ... PCR fragments were cleaned up using NucleoSpin gel and PCR clean-up kit (740609.50, MACHEREY-NAGEL) according to the users’ manual and subjected to next generation sequencing (NGS) ...
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bioRxiv - Evolutionary Biology 2023Quote: Whole DNA was extracted from fecal samples following the optimized protocol of (79) using the Genomic DNA from soil kit (NucleoSpin, Macherey-Nagel). Two successive extractions were done and a purification step was added to retrieve high molecular weight DNA suitable for long-read sequencing (79) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The total RNA of the brain samples was extracted according to the manufacturer’s protocol by applying the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The quality and quantity of RNA were assessed at 260 nm using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Neuroscience 2023Quote: ... Maxi kit for DNA from blood (Macherey-Nagel) or QIAamp® DNA Mini (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on 1% agarose gel and purified by NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel). 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel # 740902 ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction mixture was diluted 5-fold with the NTC buffer (Macherey-Nagel), and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2023Quote: Total RNAs from cell lysates were extracted using the NucleoSpin RNA II kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA were isolated for each brain area or lung tissues from the selected animal tissues using the NucleoSpin TriPrep kit (Cat# 740966.50, Macherey-Nagel GmbH & Co.KG, Germany), following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... After adding 30 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (Macherey-Nagel, cat. no 701270.510) samples were further incubated for 30 minutes at 45 °C under continuous shaking followed by GC-MS analysis.
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bioRxiv - Microbiology 2023Quote: ... RNA was purified with RNA clean-up kit (Macherey-Nagel).