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Citations for Macherey-Nagel GmbH :
1051 - 1100 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... purified using Nucleospin kit (Macherey-Nagel, Düren, Germany), and subcloned into pGEM-T plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified using Nucleospin kit according to manufacturer’s instructions (Macherey-Nagel, Düren, Germany), and visualized by electrophoresis using 2.5 % LE agarose gel ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: Minipreps were performed using NucleoSpin® Plasmid EasyPure Kits (Macherey-Nagel, Germany). 1 % agarose gels were run at 100 V for 20-30 min using 6x DNA Gel Loading Dye and GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... and the resultant powder was transferred and redissolved in lysis buffer provided by the Nucleospin Plant II kit (Macherey-Nagel, Clonetech, CA, USA). Genome isolation was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from the resulting immunopurified material was extracted using NucleoSpin RNA XS kit (Macherey-Nagel, 740902) in 100 μL RA1 lysis buffer and purified according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified using magnetic beads (NucleoMag NGS Clean-up and Size Select, Macherey-Nagel) according to the manufacturer’s instructions in a DNA to beads volume ratio of 1:1.8 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified using NucleoBond PC 2000 plasmid megaprep kit (Macherey-Nagel, #740549). Recombinant protein expression was carried out at the Protein Expression Laboratory ...
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bioRxiv - Microbiology 2023Quote: ... or the mucosal lining using a NucleoSpin RNA isolation kit (Macherey-Nagel), 0.5-1 μg was reverse transcribed into cDNA (SuperScript IV VILO Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... and 2.5 µl of RNAse (Macherey-Nagel) at 1000 unit and 3.5 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was purified with the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). The binding of cAMP-CRP (kindly provided by Annie Kolb ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Microbiology 2023Quote: ... Both products were gel-purified using the NucleoSpin Gel and PCR clean-up system (Macherey-Nagel) and stored in 5 mM Tris-HCl pH 8.5 at-30°C ...
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bioRxiv - Immunology 2023Quote: RNA extraction of in vitro cultured Th17 cells was performed with the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... PCR products were cleaned using PCR clean-up kit (Macherey-Nagel REF#740609) and validated after migration on BioAnalyser (Agilent) ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was extracted using the Nucleo-Spin RNA II kit (Macherey-Nagel). 1,000 ng of total RNA was used as input for cDNA synthesis using High- capacity cDNA Reverse Transcription kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... one of which was treated with 20 µg/mL of Proteinase K (740506.75, Macherey-Nagel), and incubated at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA of progeny virions was extracted from supernatants using the NucleoSpin RNA virus kit (Macherey-Nagel, #740956) according to the manufacturer’s instructions and stored at −80 °C until PCR-based analysis.
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bioRxiv - Cell Biology 2023Quote: ... medium-scale plasmid preparations were produced using the NucleoBond Xtra Midi Kit (Macherey-Nagel).
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bioRxiv - Immunology 2023Quote: ... Samples were later processed for total RNA isolation with NucleoSpin RNA (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from the isolated organs and conserved in 1ml NucleoProtect RNA (Macherey-Nagel, Germany). Samples were later processed for total RNA isolation with NucleoSpin RNA (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µg of ground mycelium was used for extraction of high molecular weight DNA with the NucleoBond HMW-Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... total RNA was extracted from two pools of four entire emerging female wasps using the NucleoSpin®RNA kit (Macherey-Nagel). Total RNA was finally resuspended in 40µL of RNAse free water ...
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bioRxiv - Biochemistry 2023Quote: ... The RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel). For quantitative PCR (qPCR) ...
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bioRxiv - Plant Biology 2023Quote: RNA samples were extracted using the Nucleospin plant RNA extraction kit (Macherey-Nagel GmbH & Co. KG, Germany). cDNAs were synthesized using PrimeScript RT Mastermix (Takara Bio Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted with a NucleoSpin (Macherey-Nagel) RNA extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... coli bacterial cultures using NucleoSpin Plasmid QuickPure or NucleoBond Xtra MidiPrep kits (Macherey-Nagel, Düren, Germany) according to manufactureŕs instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and NucleoBond Xtra Midi kit (Catalog no. 740410.50, Macherey-Nagel).
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bioRxiv - Evolutionary Biology 2023Quote: ... around 20 mg was digested in 200 µL of lysis buffer (NucleoMag™ Tissue from Macherey-Nagel) and 25 µL of proteinase K at 56°C ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from cells using Nucleospin RNA (Macherey-Nagel; 740955) and cDNA was synthesized from 1 μg of total RNA using the M-MLV (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Large RNA above 200 nucleotides were isolated from macrophage samples using the NucleoSpin miRNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... we extracted small RNA using the NucleoSpin® miRNA Kit (Macherey-Nagel, DuiJren, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA were extracted at different germination timepoints and in different tissues using the NucleoSpin® RNA Plant and Fungi Kit (Macherey Nagel, DuiJren, Germany) as described above but including a DNAse treatment (Macherey-Nagel, rDNAse set, DuiJren, Germany). RNA were quantified using a using a NanoDrop ND-1000 (NanoDrop Technologies ...
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bioRxiv - Plant Biology 2023Quote: RNA was isolated from 6-days-old seedlings using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The total RNA of the brain samples was extracted according to the manufacturer’s protocol by applying the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The quality and quantity of RNA were assessed at 260 nm using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Pathology 2023Quote: ... total RNA preparation was performed using the NucleoSpin RNA Plus kit (MACHEREY-NAGEL). cDNA was synthesized using random hexamer and SuperScript III (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Maxi kit for DNA from blood (Macherey-Nagel) or QIAamp® DNA Mini (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with rDNAse (Macherey-Nagel). RNA purity ...
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bioRxiv - Plant Biology 2023Quote: ... Established plasmids were extracted by NucleoBond Xtra Maxi (Macherey-Nagel, Düren, Germany). Barley cultivar GP was employed as a recipient of transgenes because barley epidermis could be peeled off more easily than wheat epidermis for releasing protoplasts ...
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bioRxiv - Plant Biology 2023Quote: ... then added 1.2 µL of RNase (MN, Macherey-Nagel, Ref. 740505, 1200 U/mL, 10 mg/mL), and transferred the DNA to a 96-well KingFisher Flex plate (ThermoFischer Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Evolutionary Biology 2023Quote: Whole DNA was extracted from fecal samples following the optimized protocol of (79) using the Genomic DNA from soil kit (NucleoSpin, Macherey-Nagel). Two successive extractions were done and a purification step was added to retrieve high molecular weight DNA suitable for long-read sequencing (79) ...
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bioRxiv - Immunology 2023Quote: ... PCR fragments were cleaned up using NucleoSpin gel and PCR clean-up kit (740609.50, MACHEREY-NAGEL) according to the users’ manual and subjected to next generation sequencing (NGS) ...