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Citations for Macherey-Nagel GmbH :
951 - 1000 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... Total DNA was extracted using NucleoSpin blood mini kit (MACHEREY-NAGEL). sgRNA was amplified by PCR using mixed P5 primer and P7 primer with i7 index sequence ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA of tick organs and eggs was extracted using the NucleoSpin Tissue kit according to the manufacturer’s instructions (Macherey-Nagel, Germany). DNA was eluted with 50 μL of elution buffer and was stored at -20°C until use.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Nucleospin RNA kit (Macherey-Nagel). Assessment of rRNA integrity and RNA-sequencing was performed by the Genome Technology Access Center at Washington University in St ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted with the NucleoSpin II kit (Macherey-Nagel) and reverse-transcribed using the High- Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing was carried out on an Illumina HiSeq XTen at Macrogen ...
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bioRxiv - Bioengineering 2023Quote: ... gDNA was obtained using the NucleoBond HMW DNA kit (Macherey-Nagel) as per manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...
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bioRxiv - Biochemistry 2023Quote: ... and 51μm filters using Macherey-Nagel a NucleoMag RNA kit (Macherey-Nagel GmbH & Co.KG). Cleared lysate was loaded into a 96 deep-well plate and put on an epMotion 5075 TMX liquid handler to complete the RNA extraction following the Machery-Nagel standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting amplicons were pooled and gel purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Samples were sequenced on a MiSeq platform with ∼10 million reads per sample (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from skeletal muscle using NucleoSpin RNAXS kit (Macherey-Nagel). Single-stranded cDNA was synthesized from 1 μg of total RNA using the High Capacity RNA-to-cDNA Kit (Applied biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted and purified with Nucleobond Xtra Maxi EF kit (Macherey-Nagel) and used to produce lentiviruses with an average titer of 109 TU/ml ...
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bioRxiv - Bioengineering 2023Quote: ... 83 RNA was extracted from MCF7 cultures (NucleoSpin RNA, Macherey-Nagel) and the quality was checked using a NanoDrop One spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from fresh bacterial cultures using the NucleoSpin® 96 Tissue Core Kit (Macherey-Nagel, Germany). All DNA samples were sequenced at the Max Planck-Genome-Centre Cologne using the Illumina paired-end technology with read lengths of 150 bp ...
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bioRxiv - Bioengineering 2023Quote: ... The liquid samples were filtered over a 0.20 μm Chromafil® Xtra filter (Macherey-Nagel, PA, USA), and stored at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: The RNA extraction and conversion to cDNA of tomato leaves was carried out using columnkit based on silica membranes (Macherey-Nagel XXX GmbH, Germany) following the manufacturer’s protocol ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from the input sample and beads by adding 300 μl buffer A1 (NucleoSpin RNA kit, Macherey-Nagel) and 300 μl absolute ethanol followed by RNA extraction according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from T-REx-293 and A549 cells using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel). 500 ng of total RNA was used for cDNA synthesis using Maxima™ Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were run on bis-acrylamide gels and then transferred to PVDF membranes (Macherey-Nagel, Düren, Germany) using a Trans-blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Maxiprep was performed according to the manufacturer’s instructions (NucleoBond® Xtra Maxi Plus EF, Macherey-Nagel), and ...
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bioRxiv - Cell Biology 2023Quote: ... Medium pH was evaluated by using a pH test paper Duotest pH 5.0-8.0 (Macherey-Nagel). We found that pH was not altered by lactate addition up to 20 mM (data not shown).
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bioRxiv - Cell Biology 2023Quote: Total RNA extraction was performed using the NucleoSpinRNA kit (Macherey-Nagel) as specified by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: RNA was extracted from cultured cells using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cssDNA released from phage were then extracted with NucleoBond Xtra Midi EF kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NucleoSpin RNA kit (Macherey-Nagel) including DNase treatment according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 300 mg (JFC) of both fresh and treated frass (n = 3) using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HPLC-grade acetonitrile was purchased from Macherey-Nagel. Formvar carbon film on 200-mesh copper grids (FCF200-Cu ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
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bioRxiv - Microbiology 2023Quote: ... viminalis roots using the Nucleo Spin® Soil kit (Macherey-Nagel, Germany). Quantitative determination of the concentration (ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Microbiology 2023Quote: ... the NucleoSpin RNA Virus (Macherey-Nagel GmbH) or by semiautomated RNA extraction using the Easymag (BioMerieuxTM) ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from strains using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). The preparation of the transposon mutant library for Oxford Nanopore Sequencing has been done as described by Linder et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Synthetic Biology 2023Quote: ... or NucleoSpin Plasmid kit (Macherey-Nagel). The correct assembly of all plasmids was verified by Sanger sequencing (Microsynth Seqlab).
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bioRxiv - Synthetic Biology 2023Quote: ... or the Nucleospin gel and PCR clean-up kit (Macherey-Nagel). Restriction and ligation reactions were performed using enzymes and buffers obtained from New England Biolabs and Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... The column was an EC150/4.6 NUCLEODUR C18 gravity column (Macherey-Nagel GmbH and Co. KG, Germany), the mobile phase was Acetonitrile:0.2 % (v/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared by adding acetonitrile ...
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Systems Biology 2023Quote: ... and 230 μg of DNA was extracted using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). Efficiency of the re-circularization was checked by Sanger sequencing of eight clones ...
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bioRxiv - Systems Biology 2023Quote: ... The pellet was washed once with sterile H2O and then each library was purified over two columns of the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). A total of 17 Mio were harvested ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted using either a NucleoSpin Tissue Kit (Macherey-Nagel) or the phenol-chloroform protocol [50] ...
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bioRxiv - Genomics 2023Quote: ... with 300 µl of Lysis Buffer T1 (Macherey-Nagel, Düren, Germany). The insect was homogenized with a pestle using a vertical motion ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted using the NucleoSpin 96 Plant II Kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...