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Citations for Macherey-Nagel GmbH :
851 - 900 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from strains using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). The preparation of the transposon mutant library for Oxford Nanopore Sequencing has been done as described by Linder et al ...
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bioRxiv - Microbiology 2023Quote: ... The filters were then individually placed into 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel), added with 1,200 µL MWA1 lysis buffer and stored at - 80°C until nucleic acids extraction as specified in the corresponding section below.
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoMag® DNA/RNA Water kit for water and air sample (Macherey-Nagel, Hoerdt, France), referred to as the “Water” ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted in triplicate for mixed cell suspensions using either NucleoMag® DNA/RNA Water (Macherey-Nagel, Hoerdt, France), i.e. ...
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bioRxiv - Molecular Biology 2023Quote: ... and column-purified using NucleoSpin RNA kit (Macherey-Nagel). 500ng of total RNA was used for reverse transcription using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cell pellets using NucleoSpin RNA kit (Macherey-Nagel). First-Strand cDNA synthesis was performed using the SuperScript III Reverse Transcriptase kit (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA from CD11B+ cells was extracted using an RNA XS Plus extraction kit and that from brains using a NucleoSpin RNA extraction kit according to the manufacturers’ protocol (Macherey-Nagel®). Reverse transcription was performed using 350 ng (CD11B+ ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was isolated from cells using the NucleoSpin Tissue kit (Macherey-Nagel). 500 ng of genomic DNA was subject to bisulfite convertion using the EZ DNA Methylation-Gold kit (Zymo) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was prepared with the NucleoSpin Microbial DNA Kit (Macherey-Nagel) and treated with 1 µL of RNase Cocktail™ Enzyme Mix (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of Glutathione Sepharose 4B (Macherey-Nagel) (75% ...
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bioRxiv - Genomics 2023Quote: High molecular weight DNA was extracted from blood cells using a NucleoBond AXG column (Macherey-Nagel, Düren, Germany), which was followed by purification with phenol-chloroform ...
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bioRxiv - Cancer Biology 2023Quote: Cells were washed with PBS and mRNAs were extracted using NucleoZOL (MACHEREY-NAGEL, 740404.200) according to the manufacturer’s instructions but the step with water was repeated twice to avoid contamination by genomic DNA ...
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bioRxiv - Bioengineering 2023Quote: ... supernatant was removed and cell pellets were used for plasmid isolation by using NucleoBond□ Xtra Midi Plasmid DNA Purification Kit (Macherey-Nagel) according to the protocol supplied by the kit.
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bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...
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bioRxiv - Biochemistry 2023Quote: ... and 51μm filters using Macherey-Nagel a NucleoMag RNA kit (Macherey-Nagel GmbH & Co.KG). Cleared lysate was loaded into a 96 deep-well plate and put on an epMotion 5075 TMX liquid handler to complete the RNA extraction following the Machery-Nagel standard protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled library plasmids were purified using NucleoBond PC 500 Maxi kit (Macherey-Nagel). The same protocol was applied to generate the library for the second round of selection ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion proteins were purified with Protino Glutathione Agarose 4B (Macherey-Nagel) and GST was cleaved by human thrombin (Biopharm laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... The thin layer chromatography was done with silica gel on aluminum foils (60 Å pore diameter) obtained from Macherey-Nagel and visualized with ultraviolet light (λ = 254 and 365 nm) ...
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bioRxiv - Biochemistry 2023Quote: ... The preparation was purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA preparation was used for transformation into chemically competent E ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids were isolated using the NucleoSpin Plasmid Kit (Macherey-Nagel) and sequenced by Eurofins using pT7-7_Seq_F and pT7-7_Seq_R as sequencing primers (Table S4) ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Bioengineering 2023Quote: ... The ligation was purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) and used for the electroporation of ElectroMAX DH5α-E Competent Cells (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... gDNA was obtained using the NucleoBond HMW DNA kit (Macherey-Nagel) as per manufacturer’s guidelines ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... Total DNA was extracted using NucleoSpin blood mini kit (MACHEREY-NAGEL). sgRNA was amplified by PCR using mixed P5 primer and P7 primer with i7 index sequence ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from fresh bacterial cultures using the NucleoSpin® 96 Tissue Core Kit (Macherey-Nagel, Germany). All DNA samples were sequenced at the Max Planck-Genome-Centre Cologne using the Illumina paired-end technology with read lengths of 150 bp ...
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bioRxiv - Bioengineering 2023Quote: ... The liquid samples were filtered over a 0.20 μm Chromafil® Xtra filter (Macherey-Nagel, PA, USA), and stored at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted using NucleoSpin (Macherey-Nagel™ NucleoSpin™ RNA, Mini Kit) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 6His-AtREM1.21–118 was produced like GST-CPK and purified on Protino® Ni-TED column following manufacturer’s instructions (Macherey-Nagel). After elution with 40-250 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: RNA isolation was performed by using the NucleoSpin® RNA kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was purified using Nucleospin® Gel and PCR clean-up kit (Macherey-Nagel # 740609), quantified by Qubit (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Extraction of genomic DNA was performed using the NucleoSpin@ Tissue kit by Macherey-Nagel, following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated using the Nucleospin RNA plus kit (Macherey-Nagel, cat# 740955.50). Library generation was performed using KAPA RNA HyperPrep Kit with RiboErase (HMR ...
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bioRxiv - Cancer Biology 2023Quote: ... The library plasmids were extracted using the NucleoBond Xtra Maxi EF Kit (Macherey-Nagel) to avoid endotoxin contamination ...
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bioRxiv - Bioengineering 2023Quote: ... 83 RNA was extracted from MCF7 cultures (NucleoSpin RNA, Macherey-Nagel) and the quality was checked using a NanoDrop One spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Nucleospin RNA kit (Macherey-Nagel). Assessment of rRNA integrity and RNA-sequencing was performed by the Genome Technology Access Center at Washington University in St ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting amplicons were pooled and gel purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Samples were sequenced on a MiSeq platform with ∼10 million reads per sample (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from skeletal muscle using NucleoSpin RNAXS kit (Macherey-Nagel). Single-stranded cDNA was synthesized from 1 μg of total RNA using the High Capacity RNA-to-cDNA Kit (Applied biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted and purified with Nucleobond Xtra Maxi EF kit (Macherey-Nagel) and used to produce lentiviruses with an average titer of 109 TU/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated (Macherey-Nagel, 740420.50) and the sequences were checked with sequencing (LGC Genomics GmbH ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted (NucleoSpin RNA-purification kit, Macherey-Nagel Dueren, Germany) and retrotranscribed cDNA quantified using the Universal Human Probe Roche library (Roche-Diagnostics ...
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bioRxiv - Cell Biology 2023Quote: ... Viral RNA in supernatant cell culture was isolated with the Nucleospin Dx virus (Macherey-Nagel). Reverse transcription was performed with random primers and Superscript IV (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: Isolation of total RNA was performed using the NucleoSpin RNA II kit (Macherey-Nagel, cat. # 740955.50) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total cellular RNA was extracted with the nucleospin RNA plus XS (Macherey-Nagel). Viral RNA in supernatant cell culture was isolated with the Nucleospin Dx virus (Macherey-Nagel) ...
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bioRxiv - Microbiology 2023Quote: ... We filtered the cultures through regenerated cellulose membrane filters (CHROMAFIL RC, 0,2 µm, Macherey-Nagel, Düren, Germany) by centrifugation (6’200 rpm for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from the resulting pellets using the NucleoMag DNA Kit (Macherey-Nagel, Allentown, PA, USA) with the aid of the AutoPure96 robot for the purification step ...