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Citations for Macherey-Nagel GmbH :
851 - 900 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: The extracted RPE or retina tissue was sonicated with lysis buffer from NucleoSpin RNA Plus Kits (MACHEREY-NAGEL, 740,984.250). Then ...
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bioRxiv - Genetics 2024Quote: ... one-half of the lysates was purified to prepare genomic DNA using NucleoSpin Tissue Kits (MACHEREY-NAGEL) and the other half was purified to prepare RNA using NucleoSpin RNA Plus Kits (MACHEREY-NAGEL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We grew plants in the greenhouse and extracted DNA from young leaf tissue using NucleoSpin Plant II kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Colonies were scraped and plasmid DNA was prepared (NucleoBond Xtra Maxi EF, Macherey-Nagel). To confirm library representation and distribution ...
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bioRxiv - Cell Biology 2024Quote: ... Ligated plasmid product was purified first by PCR-purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and then by isopropanol precipitation in the following reaction ...
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bioRxiv - Cell Biology 2024Quote: ... and gel-purified (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). The following library vectors were used ...
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bioRxiv - Cell Biology 2024Quote: ... was purchased from Thermo Fisher and TSIM (cat. #MN701310.110) was purchased from Macherey-Nagel. The triterpenoid standards diplopterol (cat ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from cells using the Macherey-Nagel Nucleospin RNA purification kit (catalogue#: 740955.50, Macherey-Nagel) following the manufacturers methods ...
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bioRxiv - Cell Biology 2024Quote: ... Amplicons were PCR-purified (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Library vector was pre-digested with FastDigest Esp3I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Amplicons were first gel-purified and then re-purified by PCR-purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Samples were sequenced on a NextSeq2000 (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or NucleoSpin Gel & PCR Clean-up (Macherey-Nagel) (Tbx2/3 pCREs ...
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bioRxiv - Evolutionary Biology 2024Quote: Viral RNA was extracted using the NucleoSpin® RNA Kit (Macherey-Nagel, Düren, Germany) directly from nasal swab supernatants ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Size selection was performed using NucleoMag™ magnetic separation beads (Macherey-Nagel, Düren, Germany) targeting an insert size of 300 bp ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA was extracted from individual frozen tissue samples using the NucleoSpin tissue kit (Macherey-Nagel®) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: All final plasmids were purified using the NucleoBond Xtra Maxi kit (Macherey-Nagel) and confirmed by restriction digests and Sanger sequencing and are listed in Table S12 ...
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bioRxiv - Genetics 2024Quote: ... and suspended in tubes in a rack of 96 tube strips (Macherey-Nagel Ref 740637) containing 375 µL of ddH20 with 5mg/mL 20T zymolyase (Euromedex ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated from cell pellets using the RNA extraction kit (Macherey-Nagel) and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Cell Biology 2024Quote: RNA from cell culture supernatant was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative PCR (RT-PCR ...
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bioRxiv - Cell Biology 2024Quote: RNA extracts were collected using the NucleoSpin RNA Plus kit (740984.250; Macherey-Nagel), and the concentration was measured using a NanoDrop Lite Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... Mini kit for RNA from plants and fungi (Macherey-Nagel, Düren, Germany). Strand-specific 3-prime mRNA libraries were prepared using BrAD-seq (Townsley et al. ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted from fresh leaves using the NucleoSpin Plant II Mini kit for DNA from plants (Macherey-Nagel, Duren, Germany) and sequenced using the HiSeq 4000 sequencing system (Illumina Inc. ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Dü ren, Germany) and send to Eurofins Genomics for Sanger sequencing of both strands ...
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bioRxiv - Developmental Biology 2024Quote: Cell total RNA was extracted using the RNANucleoSpin RNA extraction kit (Macherey-Nagel). After the cell lysis in the RA1 solution + 1% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2024Quote: ... The linearized product was purified according to manufacturer specifications via gel selection using NucleoSpin (Macherey-Nagel, 740588.50). 500 ng of DNA was used in an overnight IVT reaction using mMESSAGE mMACHINE T7 ULTRA Transcription Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated using NucleoSpin RNA Plus kit (Macherey-Nagel, 740984), and reverse-transcribed using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were purified using Nucleobond Xtra Midi EF kit (Macherey-Nagel, #740420) before transfection.
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bioRxiv - Evolutionary Biology 2024Quote: ... For the final purification with magnetic beads (NucleoMag NGS kit, Macherey-Nagel, Düren, Germany), we used a ratio of 0.65:1 bead solution to the library to discard small fragments originating from heteroduplexes that had passed the size selection ...
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bioRxiv - Developmental Biology 2024Quote: ... dsDNA repair templates were amplified by PCR using primers with containing 5′ SP9 modifications (IDT) and purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.250) prior to injections ...
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bioRxiv - Developmental Biology 2024Quote: ... A large quantity of DNA plasmids was purified using the NucleoBond Xtra Midi DNA preparation kit (MACHEREY-NAGEL).
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bioRxiv - Cell Biology 2024Quote: ... The cloned gRNA + pSpCas9(BB)-2A-Puro V2.0 constructs were then isolated from the bacterial culture using a plasmid isolation kit (Macherey-Nagel, #740588.50). Following isolation ...
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bioRxiv - Microbiology 2023Quote: ... all isolates were grown overnight at 20 °C in liquid Zobell medium and bacterial DNA was extracted using the Nucleospin tissue kit following the manufacturer’s instructions (Macherey-Nagel).
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bioRxiv - Plant Biology 2023Quote: The Protino® Ni-NTA column (Macherey-Nagel, Düren, Germany) was used for affinity chromatography of recombinant CAM2-His6 from E ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid library was finally purified by NucleoBond Midi-prep Kit (Macherey-Nagel #740410) and stored at –20°C before use ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid library was finally purified by NucleoBond Midi-prep Kit (Macherey-Nagel #740410) and stored at –20°C before use ...
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bioRxiv - Systems Biology 2023Quote: ... resuspended in sterilized water and filtered under vacuum using a 0.2 µm pore size filter (predried and preweighted; PORAFIL® MV; Macherey-Nagel). The filter was once more dried (overnight 12-24 h in an oven at 60⁰C ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Protino® Glutathione Agarose 4B (Macherey-Nagel) was used ...
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bioRxiv - Biophysics 2023Quote: ... Clean up of the PCR products was done using a standard cleanup protocol (NucleoSpin Gel and PCR cleanup, Macherey-Nagel GmbH). 2 ...
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bioRxiv - Biophysics 2023Quote: ... The 600 bp DNA was extracted from the gel using a standard NucleoSpin Gel and PCR cleanup (Macherey-Nagel GmbH). The fluorescence of the doubly labelled 600 bp band was visualized using a Typhoon 9400 scanner (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... using the PCR and gel clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturer’s protocol (Macherey-Nagel). Sanger sequencing was carried out on purified plasmid vector to validate the sgRNA incorporation ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA was extracted according to the manufacturer’s instructions (NucleoSpin® RNA, MACHEREY-NAGEL) and measured by NanoPhotometer®N60 (IMPLEN) ...
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bioRxiv - Physiology 2023Quote: ... Tissues were harvested and directly transferred into RNA stabilization solution (NucleoProtect® RNA, MACHEREY-NAGEL) overnight at 4 degrees and then stored in -80 degrees ...
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bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
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bioRxiv - Cell Biology 2023Quote: ... and purified with a silica-based resin column (MACHEREY-NAGEL, protino Ni-IDA). Aliquots were flash frozen in liquid nitrogen and stored at –80°C ...
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bioRxiv - Immunology 2023Quote: ... and ADSCs were lysed in the plate using in lysis buffer containing TCEP (Macherey-Nagel). Lysed cells were snap frozen in nitrogen and stored at -80°C until use.
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bioRxiv - Immunology 2023Quote: ... and neutrophils were harvested by gentle pipetting to asses activation status and phosphorylation of STAT3 or lysed in lysis buffer containing TCEP (Macherey-Nagel) for RNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated with the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA extraction was performed using NucleoSpin RNA Kit (Macherey-Nagel) following the manufacturer’s instructions ...