Labshake search
Citations for Macherey-Nagel GmbH :
801 - 850 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
-
bioRxiv - Plant Biology 2023Quote: ... thaliana 5-days-old seedlings was isolated using NucleoSpinTM RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... 700ng of the plasmid library was isolated from the liquid culture using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany).
-
bioRxiv - Physiology 2023Quote: ... We prepared a large amount of these plasmid vectors using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2023Quote: RNA was extracted from 50 mg of frozen and fine ground rosette leaf material from four biological replicates per genotype and growth condition using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmids were isolated using NucleoBond Xtra Midi EF kit (Macherey-Nagel) according to the manufacturer’s instruction ...
-
The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
-
bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted from various mouse organs using the NucleoSpin Tissue kit (Macherey-Nagel Inc., Allentown, PA, USA). The DNAs were used for species-specific PCR with primers against human α-satellite and mouse plakoglobin genomic regions [35] ...
-
bioRxiv - Developmental Biology 2023Quote: RNA was isolated with Nucleozol™ (Macherey-Nagel, Düren, Germany) following the extraction protocol suggested by the manufacturer ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Synthons were purified with the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) following the manufacturer’s protocol using a vacuum manifold ...
-
bioRxiv - Genomics 2023Quote: Total RNA was extracted from immature inflorescences (stages 1-12) or aerial parts of 16- day-old seedlings using NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2023Quote: ... Test kits TN 220 and COD 1500 (Macherey-Nagel, Pennsylvania, USA) were used to measure the TN and COD concentration ...
-
bioRxiv - Plant Biology 2023Quote: ... using the NucleoMag® Plant kit (MACHEREY-NAGEL, Düren, German), and concentration was adjusted to 10 ng/μl before the PCR and KASP assays ...
-
bioRxiv - Microbiology 2023Quote: ... and the backbone band was cut out and isolated using NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel). Backbone and insert were ligated using T4 Ligase (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
-
bioRxiv - Biochemistry 2023Quote: ... and plasmid DNA was extracted and purified using the NucleoSpinPlasmid kit (Macherey-Nagel). Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega ...
-
bioRxiv - Microbiology 2023Quote: ... manual protocol 5.2 (Macherey-Nagel; Düren, Germany).
-
bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel, Germany) followed by a RVFV-specific RT-qPCR amplification [32] in a Lightcycler L96 (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... The cloned BAC DNA was extracted using NucleoBond Xtra BAC kit for large construct plasmid DNA (Macherey-Nagel, catalog number 740436.25). For library cloning ...
-
bioRxiv - Microbiology 2023Quote: ... the PCR product was purified using a NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel) and subjected to Sanger sequencing at Eurofins Genomics GmbH (Ebersberg ...
-
bioRxiv - Microbiology 2023Quote: ... Chromatographic separations were carried out on a reversed-phase C18 column (50×2.1 mm, 1.8-µm particle size; Macherey-Nagel GmbH, Dueren, Germany) using a CH3OH:H2O (both supplemented with 0.1% acetic acid ...
-
bioRxiv - Microbiology 2023Quote: ... Initial purification step was realized on a Protino Ni-NTA column (Macherey-Nagel) then on a butyl-Sepharose FF (Cytiva ...
-
bioRxiv - Microbiology 2023Quote: ... followed by the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany). The second 16S amplification was performed with the primers 799 forward (5’-AACMGGA TTA GAT ACC CKG-3’ ...
-
bioRxiv - Microbiology 2023Quote: ... and 16S amplicons (500 bp amplicon size) were purified by agarose gel separation using the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel). Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... NucleoSpin Soil kit (Macherey-Nagel) was used on 2.5 g sample following the kit instruction ...
-
bioRxiv - Microbiology 2023Quote: ... DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel). PCRs were performed with Phusion DNA polymerase (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... In vitro transcribed RNA was purified using the NucleoSpin RNA Clean-up kit (Macherey-Nagel). RNA concentration and purity was evaluated using the NanoDrop One (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
-
bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 350 µl of RA1 buffer of the NucleoSpin RNA kit (Macherey-Nagel) supplemented with 1 % beta-mercaptoethanol followed by passing ten times through a narrow-bore syringe (Omican-F ...
-
bioRxiv - Microbiology 2023Quote: ... DNA purification used the Nucleospin® extract II kit (Macherey-Nagel®), according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
-
bioRxiv - Microbiology 2023Quote: ... viminalis roots using the Nucleo Spin® Soil kit (Macherey-Nagel, Germany). Quantitative determination of the concentration (ng/μl ...
-
bioRxiv - Microbiology 2023Quote: Total RNA was extracted from murine splenocytes using NuceloSpin RNA plus kit (Macherey-Nagel, France) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2023Quote: ... and extracted with the NucleoBond High Molecular Weight DNA Kit (Macherey-Nagel, Düren, Germany). The extraction yielded 16.5 μg of high-molecular weight DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
-
bioRxiv - Genomics 2023Quote: ... and construct isolation with the NucleoSpin Plasmid kit (Macherey-Nagel, REF 740588.250), plasmids were verified with sanger sequencing.
-
bioRxiv - Neuroscience 2023Quote: ... coli was done using NucleoSpin Plasmid (MACHEREY-NAGEL). The mutations were verified by sequencing (Microsynth ...
-
bioRxiv - Molecular Biology 2023Quote: ... or Nucleobond Xtra Midi (Macherey-Nagel, Germany) and each construction was verified by Sanger sequencing (Mix2seq ...
-
bioRxiv - Molecular Biology 2023Quote: ... HPLC-grade acetonitrile was purchased from Macherey-Nagel. Formvar carbon film on 200-mesh copper grids (FCF200-Cu ...
-
bioRxiv - Molecular Biology 2023Quote: Genomic DNA was isolated from cells using the NucleoSpin Tissue kit (Macherey-Nagel). 500 ng-1 μg of genomic DNA was bisulfite converted using the EZ DNA Methylation-Gold kit (Zymo) ...
-
bioRxiv - Molecular Biology 2023Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
-
bioRxiv - Microbiology 2023Quote: ... the NucleoSpin RNA Virus (Macherey-Nagel GmbH) or by semiautomated RNA extraction using the Easymag (BioMerieuxTM) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and processed using the NucleoSpin RNA kit (Macherey-Nagel) or the PureLink RNA Mini Kit (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... The correct bacterial cultures were processed the following day using the NucleoBond Xtra Midi kit for transfection-grade plasmid DNA (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and 300 mg (JFC) of both fresh and treated frass (n = 3) using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) and following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
-
bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...