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Citations for Macherey-Nagel GmbH :
751 - 800 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: We extracted genomic DNA using the NucleoSpin Plant II kit (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... the NucleoSpin RNA kit from Macherey-Nagel (cat. 740990.50) was used ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA was extracted from 2.5 grams of the two youngest leaves from a single plant of each accession using the NucleoBond HMW DNA extraction kit (Macherey-Nagel, Germany). The kit protocols were followed according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: DNA was extracted from whole insect larvae using the Nucleospin Tissue kit (Macherey-Nagel), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Plasmids were isolated using the Nucleo Spin Plasmid Quick Pure protocol (Macherey-Nagel) and PCR purified using the Nucleo Spin Gel and PCR Clean-up Kit (Macherey-Nagel ...
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bioRxiv - Immunology 2024Quote: ... the band of correct size was excised under 70% UV light using a clean scalpel and DNA was extracted using Nucleo Spin Gel and PCR Clean-up Kit (Macherey-Nagel). DNA sequences were confirmed by sequencing ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultivated in LB medium containing 50 µg/ml Kanamycin and plasmids were isolated using the Nucleo Spin Plasmid Quick Pure protocol (Macherey-Nagel).
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bioRxiv - Immunology 2024Quote: ... and PCR purified using the Nucleo Spin Gel and PCR Clean-up Kit (Macherey-Nagel) before microinjection.
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bioRxiv - Genomics 2024Quote: The embryos used for RT-qPCR were directly transferred to the RA1 buffer supplemented with 2-Mercaptoethanol provided with the NucleoSpin RNA kit (Macherey-Nagel) and stored at -80°C for further RNA extraction.
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bioRxiv - Genomics 2024Quote: ... followed by RNA purification using the Nucleospin RNA kit (Macherey-Nagel). Reverse transcription of 1 µg of RNA was performed using the RevertAid First Strand cDNA Synthesis Kit (Life technologies ...
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bioRxiv - Genomics 2024Quote: ... The plasmid library was extracted using the NucleoBond Xtra Midi kit (740410.50, Macherey-Nagel).
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bioRxiv - Molecular Biology 2024Quote: ... and RNA NucleoSpin® (Macherey-Nagel GmbH & Co) Kit ...
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bioRxiv - Biochemistry 2024Quote: ... HPLC analyses were performed using 90 µL injection volume on a Dionex Ultimate 3000 equipped with a diode array detector (DAD) using a reversed-phase C18ec column (Macherey-Nagel, 5 µm particle size ...
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bioRxiv - Biochemistry 2024Quote: ... or glutathione agarose 4B beads (Macherey-Nagel). PBS supplemented with 200 mM imidazole or 10 mM reduced glutathione was used as the elution buffer for proteins with His6 or GST tags ...
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bioRxiv - Biochemistry 2024Quote: ... soluble proteins were purified by affinity chromatography on corresponding beads: Ni-NTA agarose beads (Macherey-Nagel) or glutathione agarose 4B beads (Macherey-Nagel) ...
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bioRxiv - Bioengineering 2024Quote: ... DNA was extracted using NucleoSpin Tissue Kit (Macherey-Nagel) according to the manufacturer’s protocol and conserved at -80°C for further experiments.
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bioRxiv - Biochemistry 2024Quote: All GST-fusion proteins were purified with soluble protein fractions bound to Protino Glutathione Agarose resin 4B (GSH resin, Macherey-Nagel). For Isothermal Titration Calorimetry (ITC) ...
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bioRxiv - Biophysics 2024Quote: ... according to the manufacturer’s instructions (Macherey-Nagel). Standard PCR reactions were performed using KAPATaq DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We then dissected the antennae and extracted RNA using the Nucleospin miRNA kit (Macherey-Nagel; Duren, Germany), which extracts both large RNA (>200 bp ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant plasmids were prepared from overnight cultures and purified with NucleoSpin Plasmid columns (Macherey-Nagel). The plasmids were linearized by restriction digestion with BspHI (NEB) ...
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Investigating the effects of prednisolone on behavior in mouse models of Duchenne muscular dystrophybioRxiv - Animal Behavior and Cognition 2024Quote: ... RNA was further purified using RA1 buffer (Macherey-Nagel, Düren, Germany) and 100% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... purified and cleaned (Macherey-Nagel, #740609), and 200 ng of each laccase mutant gene were transformed along with 100 ng of linearized vector into S ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were then extracted by NucleoSpin Plasmid kit (Macherey-Nagel, #740588) and sent for sequencing (Eurofins genomics) ...
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bioRxiv - Bioengineering 2024Quote: ... and lysed with RA1 buffer (Macherey-Nagel; 740955.250). RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel; 740955.250) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and the other half was purified to prepare RNA using NucleoSpin RNA Plus Kits (MACHEREY-NAGEL, 740,984.250), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from tissues or cells using NucleoSpin™ RNA isolation kit (Macherey-Nagel) with DNase treatment ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid DNA was extracted using the NucleoBond Xtra Maxi kit for endotoxin-free plasmid DNA (Macherey-Nagel, 740424.50) and eluted in 400 µL of nuclease-free water ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2024Quote: ... following one stimulation (Acute) and after six stimulations (Chronic) using NucleoSpin Blood kits (Macherey-Nagel) (Extended Data Fig ...
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bioRxiv - Genetics 2024Quote: Genomic DNA was extracted from whole blood cells using the NucleoSpin Blood kit (Macherey-Nagel) or from tissue samples by a standard method involving proteinase K digestion and isopropanol precipitation ...
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bioRxiv - Genetics 2024Quote: ... coli using Nucleospin Plasmid Mini kits (#740588.50, Macherey-Nagel). All plasmids were sequenced by whole-plasmid sequencing performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation ...
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bioRxiv - Biophysics 2024Quote: Total RNA was extracted from multicellular aggregates with the NucleoSpin RNA Kit (740955, Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel, catalog number: 740955.50) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmid was purified using a NucleoBond® Xtra Maxiprep kit (Macherey-Nagel, Düren, Germany). The plasmid was transformed into DH5⍺ competent cells (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Mini kit for RNA purification with DNA removal column from Macherey-Nagel (Ref: 740984.50) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Gel purified (Macherey-Nagel) amplicons were assembled as in-frame fusions with emGFP PCR product into a pLenti6/V5-D-TOPO vector using NEBuilder HiFi DNA Assembly (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: Total cellular RNA was extracted from near-confluent cells in 12-well plates using NucleoZOL (Macherey-Nagel), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... total cellular RNA was isolated from Huh-7.5 cells using NucleoZOL (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: RNA from PCa cells was isolated by NucleoSpin RNA isolation kit (Macherey-Nagel, Germany). Reverse transcription was done using the PrimeScript™ RT reagent Kit (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from A375-C and A375-Cx43 untreated cell lines and under BRAF/MEKi (dabrafenib and trametinib) treatment (n=4, each condition) using the Kit RNA isolation: NucleoSpin RNA (Macherey-Nagel). Quality and integrity of each RNA sample was checked using a Bioanalyzer 2100 instrument (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... RP11-1033i2-R38-R39 was prepared for transfection using Large Costruct Kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2024Quote: ... cells were used for functional assays or RNA isolation by NucleoSpin RNA isolation kit (Macherey-Nagel, Germany). In total ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using a Nucleospin RNA PlusTM Kit (Macherey-Nagel), and RNA integrity was assessed using agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... and CAP samples using NucleoSpin Blood XL kit (Macherey-Nagel) and prepared for sequencing on an Illumina HiSeq4000 as previously described21 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products (with approximately 500-600 bp in length) were then purified with NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, MN) or with NZYGelpure kit (NZYtech ...
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bioRxiv - Biochemistry 2024Quote: ... and the desired band was excised and purified using the NucleoSpin Gel and PCR Cleanup Kit (Macherey-Nagel, catalog no. 740609) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA from the cells was isolated using NucleoSpin RNA Plus (Macherey-Nagel, Düren, Germany) and was reverse transcribed using ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Cell Biology 2024Quote: ... and column purified with standard kits (NucleoSpin from Macherey-Nagel or PureLink from Invitrogen) before being assembled by homologous recombination and then transformed in electrocompetent Stbl4 or Dh5a bacteria (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenized with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...