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Citations for Bio-Rad :
4451 - 4500 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RNA was quantified by NanoDrop and 1 µg of RNA for each sample was used to generate cDNA using the iScript cDNA synthesis kit (Biorad cat. no. 1708890). Real-time PCR was performed with PowerUp SYBR Green Master Mix (Applied Biosystems cat ...
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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bioRxiv - Molecular Biology 2023Quote: ... and optic density of the bands corresponding to mouse PRM1 and PRM2 was quantified using Quantity One 1-D analysis software (BioRad, Hercules, CA, USA) to calculate the PRM1/PRM2 ratio ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1:10 dilution of cDNA was mixed with SsoAdvanced Universal SYBR Green Supermix (cat no 1725271, Bio-Rad, Hercules, CA) and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... total RNA (1 µg) was taken from the cDNA preparation using iScript™ cDNA Synthesis Kit (#1708891, Bio-Rad Laboratories, Hercules, CA, USA). Prior to cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM DTT) to a final KOAc concentration of 75 mM and incubated with hydroxyapatite Bio gel HTP (Bio-Rad #130-0402) for 45 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg RNA was synthesized to cDNA by reverse-transcription using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA; item # 1708891). Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 60 μl 1x Laemmli buffer (diluted by mixing same volume of 1 x PBS with 2x Laemmli buffer, Bio-Rad, Catalog number: #1610737EDU) was added to the beads and incubated for 10 min at 70°C ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Immunology 2024Quote: ... The suspended cells were then diluted 1:100 in PBS and counted using a TC20 automated cell counter (Bio-Rad Laboratories, Hercules CA). For the first vaccination with cda1Δ2Δ3Δ (given to CBA/J ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Plant Biology 2024Quote: ... Diluted cDNA (1:50) was used as a template for quantitative PCRs using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Real-time detection of fluorescence emission was performed on a CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used (see also Supplementary Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Eggs were subjected to experimental electroporation parameters (voltage = 50 V; pulse = 25 ms; number of pulses = 1) using the Gene Pulser Xcell Eukaryotic System (Bio-Rad, cat. 1652661). Immediately ...
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bioRxiv - Neuroscience 2024Quote: ... Then washed and incubated with the following peroxidase-conjugated secondary antibodies at room temperature for 45 min: goat anti-rabbit IgG HRP (1:3000; Bio-Rad, California, USA) and goat anti-mouse IgG HRP (1:3000 ...
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bioRxiv - Physiology 2024Quote: ... Protein was transferred onto a 0.2 µm nitrocellulose membrane in prepared 1 × Towbin buffer with ethanol (EtOH) at 1 A and 25 V for 30 min using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA). Membranes were blocked in 1:1 Odyssey blocking buffer (Li-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... linearized with SacII restriction endonuclease was electroporated into 1 × 107 ES R1 cells using the Gene Pulser II system (Bio-Rad, CA, US) (settings ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblots were probed with the primary antibodies (see table 1) followed by corresponding HRP-conjugated secondary antibody and detected using Clarity Western ECL substrate kit (Bio-Rad, Hercules, CA). Immunoblots were quantified using Image studio software by Odyssey Fc imaging system (LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... After being dried at room temperature (RT) for 1 h and gDNA was crosslinked to the membrane using GS Gene Linker (Bio-Rad, Hercules, CA), then blocked with 5% nonfat milk for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The purified RNA was quantified by NanoDrop and 1 µg of RNA for each sample was used to generate cDNA using the iScript cDNA synthesis kit (Biorad cat. no.1708890). Real-time PCR was performed with PowerUp SYBR Green Master Mix (Applied Biosystems cat ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer to nitrocellulose membrane was carried out at 25 V and 1 A for 30 min in a Trans-Blot Turbo system (Bio-Rad, Hercules, USA). Membranes were blocked in 5% milk powder/PBS for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... which then heated at 90°C for 5 min before loading onto 4-20% gel (Bio-Rad). Proteins were separated using running buffer (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μl were loaded on a Mini-PROTEAN TGX 5%–20% Tris-Glycine gel (Bio-Rad) and run in 1X Tris Glycine-SDS (KD Medical ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted with 250 μL of 5% Chelex 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... Proteins were also run on a sodium dodecyl sulphate (SDS) polyacrylamide gels (5–20% gradient; Bio-Rad) to check for purity39,40 ...
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bioRxiv - Genetics 2020Quote: ... was added to the membrane for 5 minutes before imaging using the ChemiDoc XRS+ system (Bio-Rad) or ImageQuant 800 (AMERSHAM).
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bioRxiv - Evolutionary Biology 2020Quote: ... qPCR mixes consisted of 5 μL of Sso Advanced SYBR Green Supermix (Bio-Rad, Hercules, California, USA), 1 μL of water ...
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bioRxiv - Genomics 2021Quote: ... DNA was visualized by staining in ethidium bromide (5 µg/mL) and imaging (Gel Doc XR+; BioRad).
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bioRxiv - Neuroscience 2020Quote: ... Denatured protein lysates (5 μg) were loaded in 4–20% Criterion TGX Precast Gels (Bio-Rad, 5678095) and transferred to Immobilon-FL PVDF membrane (0.45 μm pore ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated overnight at 4°C with primary antibodies dissolved in 5% Blotting-Grade Blocker (Bio-Rad). All primary antibodies and dilutions used are listed in Supplementary Table STm.1 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 to 40μg of protein were boiled at 95°C for 5 minutes in Laemmli buffer (Biorad), separated on a SDS-PAGE gel and then electrotransfered (100V ...
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bioRxiv - Microbiology 2020Quote: ... separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis on 5– 20% gels (Bio-Rad Ready Gel J), and transferred to polyvinylidene fluoride membranes (Immobilon-PSQ ...
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bioRxiv - Plant Biology 2022Quote: ... The qPCR reaction contained 5 µl of iTaq™ Universal SYBR® Green Supermix (Bio-Rad, USA), 1 µl of 5 µM forward primer ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was extracted using 250 μL of 5% Chelex 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/mL ...