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Citations for Bio-Rad :
3701 - 3750 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of RNA (1.5 µg per sample) were reverse transcribed using an iScript Advanced cDNA synthesis kit (Bio-Rad). Resulting cDNA was diluted 1:50 in ultrapure water ...
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bioRxiv - Developmental Biology 2019Quote: ... Clarified protein lysates were measured according to standard methods using a DC protein assay kit (Bio-rad, Mississauga, Ontario, Canada). Bradford assays (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... RNA quality was assessed with an Experion Automated Electrophoresis Station using the Experion RNA StdSens analysis kit (BioRad, Hercules, CA) and yield was quantified with an ND-1000 spectrophotometer (Nanodrop ...
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Genetics 2020Quote: ... Cell lysates were clarified by centrifugation at 6,000 rpm for 7 min and protein concentration was determined using a DC protein assay kit (Bio-Rad). Samples containing equal amounts of protein were brought to a total volume of 1 ml with appropriate buffer.
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bioRxiv - Physiology 2021Quote: RNA was isolated using a Macherey-Nagel NucleoSpin RNA kit and cDNA was synthesized using iScript RT Supermix (Bio-Rad). Samples were run on a Bio-Rad CFX96 Touch to assess mRNA levels of BNP ...
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bioRxiv - Biochemistry 2021Quote: The 100µl plasma was inactivated by heating at 56°C for 30 min 10 .60µl plasma was used for albumin and globulin depletion using an Aurum serum mini kit (BioRad, USA). The six depleted plasma samples were used for SWATH-MS analysis ...
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bioRxiv - Physiology 2020Quote: ... and circulating factor concentrations were measured using the following kits according to the respective manufacturer’s protocols: Bio-Plex protein array system (Biorad, UK) with one multiplex panel was used to measure total Glucagon-like peptide-1 (GLP-1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45 mM LF PVDF Transfer Kit (Bio-Rad), and blotted with anti-HA antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein transfer to the PVDF membrane was performed following manufacturer’s instructions for the membrane kit (Trans-Blot Turbo Midi 0.2 μm PVDF, 1704157, BioRad, Hercules, CA) and the transfer apparatus (Trans-Blot® Turbo™ Transfer System ...
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bioRxiv - Genetics 2021Quote: ... Immunolabelling was visualized using chemiluminescence kits (SuperSignal West Pico PLUS Chemiluminescent Substrate; ThermoScientific) on a Chemidoc MP Imaging System (Biorad) and analyzed with the ImageLab Software (version 5.2 ...
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bioRxiv - Neuroscience 2021Quote: ... A qScript cDNA Synthesis kit (QuantaBio, Beverly, MA, USA) and T100 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) were used to generate the cDNA ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was used for RNA-seq analysis or prepared for qRT-PCR using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was used as template in SYBR green qPCR supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: qPCR was performed using the iTaq universal SYBRGreen® kit following the manufacturer protocol (Bio-Rad Laboratories Inc, Hercules, CA) on an Agilent Mx3005P qPCR Systems (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... was added to the samples according to the manufacturer’s instruction and equivalent amounts of protein (30 μg as measured using the DC Protein Assay Kit (Bio-Rad) and proteins were separated on a 4 to 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Thymi isolated from Foxp3hCD2 x Rag1GFP reporter mice were homogenized and lysed using Bio-Plex Cell Lysis Kit (BioRad Laboratories), and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 100-200 ng of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad) on an Arktik thermal cycler (Thermo scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes went through a final TBST wash and then visualized on the ChemiDoc XRS+ using the Clarity Western ECL kit (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... elegans cDNA was amplified from extracted mRNA from wild type worms using the iScript Select cDNA Synthesis kit (Bio-Rad). KLP-18 cDNA was amplified from whole worm cDNA with gene specific primers and Q5 DNA polymerase ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was reverse transcribed with oligo(dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad). The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using 1 µg of total RNA and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNAs were then quantified by real time quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufacturer’s instruction and measured using the Eon High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Microbiology 2022Quote: The secretome samples were fractionated by one-dimensional SDS-PAGE with the Mini-protean® 3 kit (Bio-Rad, USA) using a 12% resolving gel and a 5% stacking gel at 200 V for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and cDNA amplification were done using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA) and a CFX96 real-time system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... according to manufacturer’s instructions and 200 ng RNA was used for cDNA synthesis using the iScript cDNA Synthesis kit (Bio-Rad). Quantitative real-time PCR was performed in technical triplicates using Power SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... viable cells were enriched using a dead cell removal magnetic kit (130-090-101, Miltenyi Biotech) and cellular viability was assessed using Trypan blue staining (0.4%; 1450013, Bio-Rad) and an automated cell counter (TC20 ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting supernatants were normalized by total protein concentration measured by the RC DC Protein Assay kit (Bio-Rad). Equal volumes of retinal lysate containing 10 μg total protein from each mouse genotype were subjected to SDS/PAGE ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... One half microgram of total RNA was reverse transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, 1708891). All TaqMan primers and probes were obtained from Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The agarose gel plugs were prepared and processed with the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). Total RNAs used to construct RNA-seq libraries were extracted from various tissues of a female juvenile zebra shark (total length ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from kidney and cells using Aurum TM Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad) or RNAqueous™ Micro Total RNA Isolation Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... One µg of total RNA was used for first-strand cDNA synthesis with the iScript cDNA Synthesis Kit (Bio-Rad) and diluted 8 times afterwards ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Microbiology 2022Quote: ... in 1x TBS buffer for 30 minutes at 250 V and transferred using the Trans-Blot turbo Mini 0.2 μm PDVF transfer kit (Bio-Rad) with a Transblot Turbo transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 50 μL of 1X PBS and protein concentration was using The DC Protein Assay Kit (Biorad).
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng DNase-treated RNA was used as template for cDNA synthesis using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad). The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA utilized in the PCR experiments was generated via the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules California #1708890). PCR Reactions were then performed in triplicate (3n ...
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bioRxiv - Neuroscience 2023Quote: ... a cDNA library was generated using 500 ng of total RNA using the iScript cDNA synthesis kit from Bio-Rad. The concentration of the cDNA was then quantified using the Qubit 2.0 assay for oligos per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were hybridised with an appropriate DNA probe— as indicated in figure legends and Table S2—radiolabelled with P32 prepared via random priming using the High Prime kit (BioRad). Pre-hybridisation solution was discarded ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed and converted to complementary DNA (cDNA) using the iScript cDNA synthesis kit (1708891, Bio-Rad, Hercules, CA). Each cDNA sample was amplified with messenger RNA (mRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of up to 1μg of total RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad, #1725038).
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse transcribed to generate cDNA using the iScript Reverse Transcriptions SuperMix for RT-qPCR kit (Bio-Rad Laboratories). Real-time PCR was performed using the SYBR select Master Mix for CFX (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellets were re-dissolved in 1 M NaOH and quantified by Bradford assay (Quick StartTM Bradford Kit, BIO-RAD), and the supernatants containing cADPR were neutralized with Chloroform/Tri-n-octylamine mixture (volume ratio 3:1 ...