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Citations for Bio-Rad :
3801 - 3850 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and IFNγ) by the Bio-Plex Pro Mouse Cytokine Assay kit with the Bio-Plex 200 systems from Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2023Quote: The total protein concentration of the ECM digests was measured using a DC protein assay kit (Bio-Rad, Watford, UK) following the manufacturer’s instruction (n = 4) ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Neuroscience 2023Quote: 500ng of RNA/sample was used to make cDNA using iScript cDNA synthesis kit according to manufacturer’s protocol (Bio-Rad). cDNA abundance was measured using 300nM of indicated primers and a iQ5 qPCR system (Bio-Rad).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... Two μg of total RNA each was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Oligonucleotide primers used for RT-PCR for each gene are listed in Supplementary Table S4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was measured using the modified Lowry assay (DCTM Protein Assay Kit, BIO-RAD, 5000111). The same amount of total protein was mixed with Laemmli sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Plant Biology 2023Quote: ... leaf tissue was ground in liquid N2 and total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Genetics 2023Quote: ... 800 ng of total RNA were used to perform the RT step using the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... The total protein concentration for each supernatant was measured with a DC protein assay kit (5000113, 5000114, 5000115; Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Total RNA was reverse transcribed with oligo (dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad). The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA used for ddPCR was transcribed using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Biorad, Munich, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified with a dsDNA HS Assay kit on a Qubit fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... TX) and 750ng of RNA was reverse transcribed to generate cDNAs using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Relative transcript levels were analyzed by real-time qPCR using SYBR Green (SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2023Quote: ... All gels were transferred to PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kits (Bio-Rad, 1704274).
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using iScriptTM cDNA synthesis kit and qRT-PCR was performed using a CFX96 Touch Real-Time PCR Detection System (BioRad). Data were analyzed using the following equations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA (1μg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Each embryo was injected with 100 pg memGFP or 200 pg mCherry mRNA ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) was created for each sample from equals amounts of total RNA using the iScript cDNA synthesis kit (1708890 BioRad). Real-time PCR was performed from cDNA on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... A part of the samples was then analyzed by a BioRad Fish Barcoding Kit (Bio-Rad Laboratories Inc., Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in the lysates was determined using the Bio-Rad Protein Assay Kit – BSA (Cat#5000002, Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... HRP is detected by a chemiluminescence assay using the kit Clarity Western ECL Substrate (Bio-Rad, Marnes-la-Coquette, France), whose light emission is captured by the camera of the device ChemiDoc Touch (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: HepG2 protein lysate was separated by SDS-Page (TGX Stain-FreeTM FastCastTM Acrylamide Kit, Bio-Rad Laboratories, Inc., Hercules, US) and blotted on nitrocellulose membranes (Trans-Blot® TurboTM ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... were transferred to a 0.45μM nitrocellulose membrane (Trans-Blot Turbo RTA Transfer kit nitrocellulose midi #1704271) using Trans-Blot Turbo Transfer System (Bio-Rad #1704150 ...
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bioRxiv - Pathology 2024Quote: ... We used 200ng of total RNA to synthesize single-stranded cDNA using iScript cDNA synthesis kit based on manufacturer’s directions (Bio-Rad). cDNA was then used for qRT-PCR analysis using SYBR Green qPCR master mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 1µg of DNase I treated RNA was converted into cDNA using an iScript cDNA synthesis kit (BIO-RAD, Cat:1708891) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed using ECL Advance Western Blotting Detection Kit (Amersham) and the chemiluminescence was detected using an ChemiDoc Imaging System (BioRad).
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bioRxiv - Microbiology 2024Quote: Cytokine/chemokine expression levels were further confirmed using the Bio-Plex Multi-Plex Assay Kit (Bio-Rad Laboratories, Hercules, CA). Supernatants from organ homogenates were used to determine presence of 22 proteins including eotaxin ...
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bioRxiv - Immunology 2024Quote: The concentration of total protein in each processed sample was quantified using the DC Protein Assay Kit II (Bio-Rad) with a standard curve (R2 > 0.99 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA samples were obtained from 1 µg RNA using the iScriptTM cDNA synthesis kit (Bio-Rad, Cat no. 170-8891) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was detected using the Clarity Western ECL kit following manufacturer protocol and imaged using a ChemiDoc XRS+ apparatus (BioRad). Pixel density for each dot was quantified using the volume tool in ImageLabTM software (BioRad).
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bioRxiv - Pathology 2024Quote: ... Total reaction volume was 10 µl and contained 5 µl of iTaq Universal SYBR green one-step kit (Bio-Rad), 300 nM of forward and reverse primers (Table S5 ...
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bioRxiv - Pathology 2024Quote: ... Hrd1 and GAPDH was quantified through one-step RT-qPCR using iTaq Universal SYBR green one-step kit (Bio-Rad) with the corresponding primiers (Table S5 ...
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bioRxiv - Microbiology 2024Quote: TNF-α and IL-10 were quantified in the collected supernatants using Bio-Plex Pro Human Luminex kit (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer) using Bio-Rad CFX Maestro 1.1 software (Bio-Rad). The primers used are listed in Supplementary table 6.
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bioRxiv - Molecular Biology 2024Quote: ... Gene-specific cDNA synthesis was carried out with 2 µg of mtRNA using the iScript Select cDNA Synthesis Kit (BioRad) and oligo 2278 (see Supplementary Table ST2) ...
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bioRxiv - Genomics 2024Quote: ... 500 ng of RNA was further reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). Semi-quantitative PCR was carried out using specific primers for the following genes ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg of total RNA were processed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; www.bio-rad.com), to achieve genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... and the RNA was reverse transcribed into cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad), as per the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was converted to cDNA using iScript advanced cDNA synthesis kit for RT-qPCR (Cat# 1725037, Biorad) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...
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bioRxiv - Immunology 2024Quote: ... cDNA was made from equivalent starting amounts for each sample and synthesized using the iScript cDNA Synthesis Kit (Bio-Rad). Total cDNA was diluted 1:10 in water and amplified by real-time polymerase chain reaction using a BioRad CFX-96 iCycler and SYBER Green Supermix ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Biorad, Hercules, California) in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...