Labshake search
Citations for Bio-Rad :
3551 - 3600 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: Retrotranscription of RNA was performed using 3μg of total RNA in a 40μl final volume reaction mixture using iScript Advanced cDNA Synthesis Kit for RT-qPCR (Biorad) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... The total RNA (1.2 μg) was used to synthesize single-stranded cDNA using iScript cDNA synthesis Kit (Bio-Rad) and T100 Thermal Cycler (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... 500ng RNA was used as input for cDNA synthesis through iScript™ advanced cDNA synthesis kit (Bio-Rad 1725038) following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... The membrane was revealed with the Clarity™ Western ECL Substrates Kit (Bio-Rad Laboratories Inc, Hercules, CA, USA) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using the iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) with 2.0-20 ng input corneal RNA according to a manufacturer protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: ... The total protein content was determined using the colorimetric Bio-Rad Protein Assay Kit II (Bio-Rad Laboratories, CA). Seven dilutions of a protein standard containing 0 to 30 µg/mL of the total protein content were used ...
-
bioRxiv - Molecular Biology 2023Quote: ... and RNA was then reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) following the manufacturers guidelines ...
-
bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed using 350 ng (CD11B+) and 1,000 ng (brain) mRNA with an iScript cDNA synthesis kit (BioRad). Real-time quantitative PCR was performed on triplicate samples using SYBR Green Supermix (BioRad) ...
-
bioRxiv - Immunology 2023Quote: ... Freshly isolated mRNA was reverse transcribed into complementary DNA with random primers by iScript cDNA Synthesis Kit (Bio-Rad).
-
bioRxiv - Molecular Biology 2023Quote: ... Signal was detected using the HCL kit (ECLTM Primer Western Blotting Detection Reagent, AmershamTM) and a Chemidoc system (Biorad). Signal were quantified with the ImageLab Software.
-
bioRxiv - Developmental Biology 2023Quote: ... total RNA samples were reverse transcripted into first-strand cDNA using the iScript Select cDNA Synthesis kit (Bio-rad). 1 µl of a 10X diluted cDNA sample was then used in the qPCR reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-rad) ...
-
bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the iScript™ cDNA Synthesis Kit and iTaq Universal SYBR Green Supermix (Bio-Rad) and run on a Biorad CFX384 real-time system (C1000 Touch Thermal Cycler) ...
-
bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from 1 µg total input RNA using iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). In a qPCR plate (Bio-Rad MLL9601) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared from 500ng RNA per reaction using the iScript cDNA Synthesis Kit (#1708891, Bio-Rad, Hercules, CA). QPCR was performed using probe-based primer sets and master mix (Prime Time ...
-
bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined by a detergent compatible DC™ Protein Assay Kit II (Bio-Rad Laboratories, #5000112). For Western blot analysis ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Standard curves were run for all primer pairs to ensure high efficiencies (>97% ...
-
bioRxiv - Plant Biology 2024Quote: The AtGCN5 coding sequence amplified from Arabidopsis Col-0 cDNA using iProof™ High-Fidelity PCR Kit (Bio-Rad) was inserted into pDONR221 vector (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... 7µl nuclease-free water and 10 µl of SSo advanced universal SYBR Green qPCR kit (Bio-Rad, Ref: 1725272) for gill derived samples ...
-
bioRxiv - Bioengineering 2024Quote: ... Sample concentrations were normalized to 1 ng/µL during cDNA preparation using the iScript Synthesis Kit (Bio-Rad, 1708841), following manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1µg total RNA per sample using a Bio-Rad cDNA Synthesis Kit (Bio-Rad Laboratory). Quantitative real-time polymerase chain reaction (RT-qPCR ...
-
bioRxiv - Cell Biology 2024Quote: cDNA was synthesized from 500 ng RNA using the iScript™-cDNA Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time quantitative PCR was carried out using mRNA-specific primers (Table S1) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Gene expression was quantified using the iTaq Universal One-Step RT-qPCR Kit (cat. 1725150, BioRad, Hercules, CA, USA) in Applied Biosystems and normalized to GAPDH or Actb ...
-
bioRxiv - Bioengineering 2024Quote: ... transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) at 25V ...
-
bioRxiv - Molecular Biology 2024Quote: ... Five hundred nanograms of total RNA were used for a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA was depleted with Mouse/Rat riboPOOL (siTOOLs Biotech) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Four hundred nanograms of total RNA were used for a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
-
bioRxiv - Neuroscience 2024Quote: ... and 0.5 μg total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad; #1708891). Real-time quantitative polymerase chain reactions (RT-qPCR ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes ...
-
bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed in triplicate with a PCR starter kit in CFX96 Real-Time System (Bio-Rad, USA). hsa-miR-16-5p was used as an internal control to normalize the gene expression ...
-
bioRxiv - Plant Biology 2020Quote: ... All samples were transferred to Nitrocellulose membrane with the Trans-Blot Turbo RTA transfer kit (BIO-RAD Cat.#170-4270). The chemiluminescent signal was detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
-
mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
-
bioRxiv - Cancer Biology 2021Quote: ... 3 µg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
-
bioRxiv - Cell Biology 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). FCGRT ...
-
bioRxiv - Cell Biology 2019Quote: Total RNA was extracted using the Total RNA Kit I (EZNA) and cDNA synthesis was carried out using iScript (Biorad) according to the manufacturers instructions and using 300 ng total RNA for each sample.
-
bioRxiv - Cell Biology 2020Quote: ... RNA (1µg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2019Quote: ... We used SuperSignal West Pico or Dura chemiluminescent detection kits (Thermo Fischer) and the chemiluminescent signal was acquired using the ChemiDoc system equipped with an XRS camera (BioRad).
-
bioRxiv - Cell Biology 2020Quote: ... 500ng of RNA was reversed transcribed into cDNA in a 20 μL reaction volume using iScript cDNA synthesis kit (Biorad) in accordance to manufacturer’s guidelines ...
-
bioRxiv - Immunology 2021Quote: ... Quantity of libraries was assessed by performing digital droplet PCR (ddPCR) with Library Quantification Kit for Illumina TruSeq (BioRad, #1863040). Libraries were diluted to 2 nM and paired-end sequencing was performed on a HiSeq 2500 sequencer (Illumina) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using 600 ng input total RNA per 40 ¼l reaction with the iScript cDNA Synthesis Kit (BioRad), cycling for 5 min at 25°C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ ECL kit (Bio-Rad) according to the manufacturer’s instructions and visualized with a CCD camera of the Odyssey Fc imaging system (Li-COR Biosciences ...
-
bioRxiv - Developmental Biology 2021Quote: ... was used to isolate the total RNA followed by cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, 1708891). Real-time qPCR quantification was done on a Bio-Rad CFX96 Real-Time system using SsoFast EvaGreen Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: RNA was harvested as described above and used to create cDNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories). qPCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufactureŕs instruction and measured using the EonTM High Performance Microplate Spectrophotometer (BioTek ...
-
bioRxiv - Neuroscience 2021Quote: ... Synthesis of cDNA was performed in duplicate from 500ng RNA in 10μL reactions using the iScript Advanced cDNA Synthesis kit (Bio-Rad). Reactions were diluted 1:5 in water ...
-
bioRxiv - Neuroscience 2020Quote: ... 500 ng of RNA was used for Realtime RT-PCR analysis using iTaq universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The RNA was then cleared of genomic DNA using DNAse from an iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad).
-
bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 65 −175 ng of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Target-specific primers designed with the assistance of the free web-based PrimerQuest® Tool (IDT ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with random primers using an iScript cDNA synthesis kit (Bio-Rad) and analyzed with a Bio-Rad CFX96 real time system ...