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Citations for Bio-Rad :
3501 - 3550 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was purified from dissected tissues using an Aurum Total RNA Fatty and Fibrous Tissue kit (Biorad 7326830) and then quantified with a Nanodrop 1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng - 1μg DNase-treated RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instruction and the resulting cDNA was used for reverse transcription-polymerase chain reaction (RT-PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Molecular Biology 2022Quote: ... and a calibration curve of protein content was created from the BSA protein standard kit (Bio-Rad Laboratories, CA). An equal amount of protein (50 ng ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis for qRT-PCR was done with iScript® gDNA Clear cDNA Synthesis kit (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Synthesis of cDNA from 100 ng input RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories). Primers were pre-designed (PrimeTime qPCR Primers ...
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bioRxiv - Biochemistry 2023Quote: ... and cells were lysed in 350 μl/well lysis buffer from the AurumTM total RNA Mini Kit (Biorad, 7326820). The lysates were immediately frozen at −80 °C until RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... Only samples with an RNA integrity score (RIN) greater than 8.0 were selected for the preparation of cDNA libraries using iScript cDNA Synthesis Kit (BioRad). One nanogram of total RNA was amplified to several micrograms of cDNA.
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bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA was synthesized from 500 ng of total RNA with the iScript cDNA synthesis kit (Bio-Rad) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were separated by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-PROTEAN kit (1658001FC, Bio-Rad). Between 20 and 50 μg of protein per well were loaded ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was transcribed into cDNA using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, Hercules, CA). Real time PCR (Polymerase Chain Reaction ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA titers and infections were assessed via qRT-PCR using an iTaq Universal Probes One-Step Kit (BioRad) in a 20 µl sample volume ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of the purified RNA was employed to synthesize cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). RT-qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... using 11 μL undiluted RNA and the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Cat# 1864021). Droplets were generated ...
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bioRxiv - Cell Biology 2023Quote: ... following the procedure of Stoeckius et al.[28] using the LYNX Rapid Streptavidin Antibody Conjugation Kit (Biorad, Ref #LNK163STR). Briefly ...
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bioRxiv - Pathology 2024Quote: ... First strand cDNA was synthesized from 500 ng RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Real-time PCR was carried out using TaqMan assays in a 10 μl reaction mixture (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto membranes using Trans-Blot® TurboTM RTA Midi 0.2 µm Nitrocellulose Transfer Kit (Biorad, #1704271). The membranes were subsequently blocked in TBST solution (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... according to manufacturer’s protocol and used for reverse transcription with iScript cDNA synthesis kit (Bio-rad, cat #170-8890) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Microbiology 2023Quote: ... NS1 antigen levels were measured using the Platelia™ Dengue NS1 Antigen kit (Cat No. 72830, Bio-Rad, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mRNA was isolated with TRIzol RNA Isolation protocol and used to make cDNA using iScriptTM cDNA Synthesis Kit (BioRad). The StepOneTM Real-Time PCR System was used to analyze the samples under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5 μg total RNA was reverse transcribed to generate cDNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription PCR was performed on the sample to generate cDNA using iScriptTM cDNA Synthesis Kit (BioRad, Hercules, USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The cDNA obtained was subjected to qRT-PCR using the itaq™ Universal SYBR green kit (Bio-Rad, #1725122) and the CFX 96 Real-Time System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was carried out with 500 ng of total RNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad Cat# 1708891). qPCR was conducted using PowerUp™ SYBRTM Green Master Mix (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Genetics 2023Quote: ... One μg of total RNA was reverse transcribed using a mix of oligo(dT) and random hexamer primers following the manufacturer’s instructions of the iScriptTM cDNA synthesis kit (BioRad). Primers to detect endogenous transcript levels of MSH2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplification of WHY1 for each Corallorhiza species was performed using the Taq RED PCR Kit from Bio-Rad Industries (Hercules ...
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bioRxiv - Genetics 2023Quote: ... Approximately 700 ng total purified RNA was used to generate cDNA with the iScript cDNA Synthesis kit (Bio-Rad). cDNA was used for PCR to quantify gene expression for SLC15A4 ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized from 1 µg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed using the SsoAdvanced™ Universal SYBR® Green RT-PCR Kit (Bio-Rad) on a CFX96 thermocycler (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: Cortical and hippocampal brain tissue sections were lysed using the Bio-Plex cell lysis kit (Bio-Rad Laboratories #171304011) and protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher #23225) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 ng of the extracted RNA was reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was then synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (1708890, Bio-Rad) and Bio-Rad T100 thermocycler ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Complementary DNA was synthesized from 0.5μg total RNA for all in vivo and in vitro experiments using the iScript cDNA kit (1708891, Bio-Rad). Five µL qPCR reactions were carried out using iTaq Universal SYBR Green Supermix (1725124 ...
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bioRxiv - Neuroscience 2023Quote: Fractionation of nuclear and cytoplasmic fractions from Drosophila lysates was conducted with the ReadyPrep Protein Extraction Kit (Bio-Rad). Seven whole flies per group were homogenized in cytoplasmic extraction buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Molecular Biology 2023Quote: ... the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).
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bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...
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bioRxiv - Microbiology 2023Quote: ... Products were purified using the ssDNA clean and concentrate kit and loaded on 10% TBE-Urea gels (BioRad #4566033) with sample buffer (Thomas Scientific #C995V39 ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: qRT-PCR was performed on a C1000 Thermal Cycler using SsoFast™ EvaGreen Supermix kit (Bio-Rad, Hercules, CA) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... The protein bands were visualized using an ECL kit (Meilunbio, CHN) and imaged on ChemiDoc XRS+ (Bio-Rad, USA).