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Citations for Bio-Rad :
3901 - 3950 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad). GAPDH was used as the invariant housekeeping gene internal control.
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg of each RNA sample was used in a 10-µL reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2020Quote: ... and the Takyon No ROX SYBR 2X MasterMix Blue dTTP qPCR Kit (both Eurogentech) for quantitative real-time PCR using a CFX96 detection system (Bio-Rad). Transcript data was normalized to UBQ10 ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were stored at −80°C until cDNA synthesis using the iScript™ gDNA Clear cDNA Synthesis Kit according to the manufacture’s guidelines (BIO-RAD). A qPCR-based assay was used to detect and quantify PfCSP transcripts (Kefi et al. ...
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bioRxiv - Genomics 2021Quote: ... 30-40 ng of RNA was used to make cDNA using the iScript Adv cDNA Syn Kit (Bio-Rad life Sciences). The RNA was diluted 1:50 in water for ddPCR analysis ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM or 3.2 μM of SAM-3H3 were used and the reaction was cleaned with either a PCR purification kit or Bio-Spin P30 columns (Bio-rad). The reactions were incubated for 2 hrs at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected using an ECL detection kit (ATTO, EzWestLumi plus, WSE-7120) with a ChemiDoc imaging system (BIO-RAD).
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bioRxiv - Microbiology 2021Quote: ... Additional one-ml samples were used for total protein determination using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). β-galactosidase assays were performed as described previously (38) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SYBR Green qPCR was performed using a ViiA7 (A&B Biosystems, Waltham, MA) with iTaq Universal SYBR Green One Step Kit (BioRad, #1725151) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... The blots were developed using enhanced chemiluminescence using Cynagen Westar Supernova detection kit and imaged using ChemiDocTM system from Bio-Rad. The blots were then stripped and re-probed for total proteins ...
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScriptTM One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR (qPCR) was carried out by using SYBR Green Supermix kit with the iCycler QTX detection system (Bio-Rad). Primers are listed in Table EV5 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was established using BCA kits (Pierce) and 10µg of protein was loaded into each well of a 4-20% pre-cast gel (BioRad, MiniProtean TGX), flanked with ladder at either end of the gel (PageRuler ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Neuroscience 2022Quote: ... we synthetized cDNA using immunoprecipitated samples from Npas1-Cre-RT and RT mice (see above for detailed procedure) using the iScript cDNA synthesis kit (Bio-Rad). cDNA was preamplified using TaqMan Gene Expression Assay system (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Three to four hundred nanograms of complementary DNA was synthesized using a reverse transcriptase complementary DNA synthesis kit (iScript, Bio-Rad). Changes in mRNA expression were measured using quantitative PCR (Biorad ...
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bioRxiv - Plant Biology 2021Quote: ... and 2 μg of the RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative RT- qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Immunology 2020Quote: ... Measurements and analyses of secreted cytokines from a murine 35-plex kit were performed using a multiplex bead-based technology (Luminex) assay with a Bio-Plex 200 instrument (Bio-Rad) after 2-fold dilution of supernatants.
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bioRxiv - Biochemistry 2021Quote: The details of the protein purification protocol for human plasma sample are described in the Aurum serum mini kit leaflet and a detailed was followed(BioRad, USA). The sample was prepared for 2-D gel electrophoresis following passive rehydration for 16-24 h ...
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bioRxiv - Plant Biology 2021Quote: ... Quality and quantity of RNA samples were assessed using the Bio-Rad Experion analyser and Experion RNA high-sense analysis kit (Bio-Rad). Total RNA from each biological replicate was amplified using the MessageAmp II aRNA amplification kit (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and the protein concentration was measured using a DC protein assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of protein (5 μg ...
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bioRxiv - Microbiology 2021Quote: ... and 30 ng of RNA from each sample was reverse transcribed using the iScript™ cDNA Synthesis Kit (1708891; Bio-Rad). Real-time PCR was performed using Applied Biosystems™ TaqMan™ Gene Expression Master Mix (4369016 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA was prepared in a 10 μl volume containing 350 ng of plant total RNA that was reverse-transcribed with an iScriptTM cDNA Synthesis Kit using modified MMLV-derived reverse transcriptase (Bio-Rad), a blend of oligo-d(T ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was quantified on a Bio-Rad T100 96-Well PCR Gradient Thermal Cycler with the SYBR Green kit (Bio-Rad, SsoAdvanced™ Universal SYBR® Green Supermix) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Lysates from the ribopuromycylation assays were loaded into gels prepared using the TGX Stain-Free FastCast Acrylamide Kit (Bio-Rad, 1610183) (Fig ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then reverse transcription was performed by HiScript III RT SuperMix for qPCR kit (R323, Vazyme, Nanjing, China) by BIO-RAD iCycler(BIO-RAD ...
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bioRxiv - Systems Biology 2022Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2022Quote: One ng of total RNA was used for single-tube reverse transcription (RT) and ddPCR using the One-Step RT-ddPCR Advanced kit (Bio-Rad). Droplets were generated and subsequently analyzed using the QX100 system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of total RNAs was used to generate cDNA using the iScript cDNA synthesis kit (Bio-Rad, Richmond, CA, USA). Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Immunology 2022Quote: ... was converted to cDNA with the C1000 Touch Thermal Cycler using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad, 1708890), with the following modifications ...
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bioRxiv - Microbiology 2022Quote: ... about 25 ng of DNA-free total RNA for each lncRNA was reverse-transcribed using iTaq cDNA Synthesis Kit (Bio-Rad) and then analyzed with ABI 7500 Fast Real-time PCR System (Applied Biosystem ...
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bioRxiv - Plant Biology 2019Quote: ... RNA samples confirmed as free of genomic DNA were diluted to equivalent concentrations and subjected to cDNA synthesis with iScript cDNA Synthesis Kit according to the manufacturer’s instructions (BioRad, Hercules, CA). All cDNA synthesis reactions for a single time-point were performed simultaneously to decrease any inherent variability in the synthesis process ...
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bioRxiv - Genomics 2020Quote: ... Concentration and 260/280 ratios were quantified using a NanoDrop 1000 spectrophotometer before cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad). Primers were designed to span exon-exon boundaries wherever possible (Supplementary Table 2) ...
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bioRxiv - Genetics 2019Quote: ... Reverse transcription of 400ng of RNA into cDNA was completed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and the Veriti 96-well thermal cycler according to the manufacturer’s instructions (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA from Astyanax embryos of various stages (2-24 hpf) was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) and amplified using the following primers:
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated with secondary antibodies for one hour at room temperature and developed using Clarity ECL Detection Kit (Bio-Rad).
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bioRxiv - Genomics 2019Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2019Quote: ... Cells were collected and resuspended in OMNI Tagmentation Mix containing ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme that are parts of the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad). The OMNI Tagmentation Mix was buffered with 1X PBS supplemented with 0.1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... was performed using 300 ng RNA with the CFx Connect Real-Time system with the iTaq Universal SYBR Green One Step Kit (Bio-Rad) according to manufacturer’s instructions ...