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Citations for Bio-Rad :
3601 - 3650 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1-0.2 μg of RNA was used to synthesize cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA template was mixed with iTAQ SYBR green universal super mix (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA (cDNA) was synthesized from 500 ng of purified RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) following protocols provided by the manufacturer.
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared and analyzed using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit (Bio-Rad, Hercules, CA). Assays were performed according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... RT-PCR reactions were carried out using the Bio-Rad One-Step RT-ddPCR Advanced Kit for Probes (BioRad, 1864021) following the manufacturer’s protocol with the following reaction conditions ...
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bioRxiv - Biochemistry 2019Quote: ... Protein content was measured using the BCA kit and 50 micrograms of protein per sample were loaded on a Bio Dot apparatus (Biorad) equipped with a 0.2 micron pore size nitrocellulose membrane soaked in buffer B (10 mM Tris-HCL pH 8.0 ...
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bioRxiv - Genomics 2019Quote: ... The exact molarity of the pools were assess by qPCR using the KAPA Library Quantification Kit Illumina on CFX96 system (Biorad). Then each pool was sequenced on 1 flowcell of HiSeq 2000 system (paired-end ...
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bioRxiv - Cancer Biology 2019Quote: ... according to the manufacture’s protocol and 1 µg RNA was used to convert to cDNA using iScript Select cDNA Synthesis Kit (Bio-Rad) in the presence of both oligo (dT ...
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bioRxiv - Molecular Biology 2019Quote: ... Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce) and imaged and quantified with a ChemiDoc MP Imaging System (BioRad). For quantification of Cas9 protein in dCas9-KRAB and dCas9-VPR parental clones ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription of 1 μg of total RNA per sample was performed using the iScript cDNA synthesis kit (Bio-RAD). Transcript levels were determined by quantitative real-time RT-PCR as previously reported [14] using primers provided in Supplementary Table 1 and normalizing expression to ATCB transcript levels.
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bioRxiv - Microbiology 2019Quote: ... The antigen-antibody reaction was analyzed by using Opti-4CN detection kit according to the instructions of the manufacturer (Biorad).
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bioRxiv - Physiology 2020Quote: ... The integrity of the extracted RNA samples was additionally determined by using an Experion RNA StdSens Analysis Kit (Bio-Rad). Any samples showing poor RNA quality were also excluded from further analysis ...
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bioRxiv - Neuroscience 2020Quote: cDNA was synthesized from total RNA isolated from LCLs by using a reverse transcription kit iScriptTM cDNA synthesis (1708891, Biorad). The reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The quality of the resulting RNA library was assessed using the Experion RNA Analysis Kit (Bio-Rad; Hercules, CA, USA), and only the samples with an integrity number 8-10 were selected for transcriptomic analysis ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System RTA kit following the manufacturer’s protocols (Bio-Rad). Subsequently ...
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bioRxiv - Molecular Biology 2019Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified using Qbit dsDNA HS Assay kit (Invitrogen) ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension is then embedded in 1% low-melt agarose plugs and treated using the CHEF Mammalian Genomic DNA Plug Kit (Biorad), according to the manufacturer’s protocol38,39 ...
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bioRxiv - Microbiology 2019Quote: ... 1 µg total RNA was subjected to random-primed cDNA synthesis using an iScript cDNA Synthesis kit following the manufacturer’s protocol (Bio-Rad). 1 µL of the resulting cDNA was used in triplicate 25-µL qRT-PCR reactions ...
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bioRxiv - Plant Biology 2019Quote: ... The amount of protein recovered after lysis was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad), with bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized from pre-TRAP and TRAP RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad, #170-8841). mRNA expression was measured using TaqMan Fast Advanced Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1µg of RNA from each sample was converted to double stranded cDNA using iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were removed at stated time points and secreted cytokine quantities were measured using Luminex Bead technology according to Bioplex protocols and cytokine Kit (Biorad).
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.5 µg of total RNA using a RevertAid Premium Kit (Fermentas) and subjected to RT-qPCR using iTaq Universal SYBR Green Supermix (BioRad) and 10 ng of cDNA per reaction ...
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bioRxiv - Neuroscience 2020Quote: ... RNA (1 μg) was reverse-transcribed to cDNA with the iScript ™ cDNA Synthesis Kit (Bio-Rad Laboratories, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of viral RNA using one-step real-time reversetranscription PCR was performed according to (Corman et al., 2020) with iTaq Universal Probes One-Step Kit (Biorad) using primers and probes against the N-gene (N_Sarbeco_F ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was synthesized from 1 μg of isolated RNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05 ...
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bioRxiv - Biochemistry 2021Quote: ... Total RNA from ddHEKs and ddMEFs were harvested utilizing the Aurum Total RNA Mini kit (Bio-Rad, Hercules, CA, USA) and DNase treated on-column ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The purified RNA was quantified and an equal amount of RNA was used for cDNA preparation using iScript cDNA synthesis kit (Biorad). GAPDH was used as a housekeeping gene was used ...
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bioRxiv - Neuroscience 2020Quote: ... We used 1 microgram of the total RNA for cDNA synthesis (iScript cDNA Synthesis Kit, Cat. #170-8891, Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Mini-PROTEAN TDX Precast Protein Gels and Trans-Blot Turbo RTA Mini PVDF Transfer Kit were purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrations of the extracts were determined using the Bio-Rad Protein assay kit (Bio-Rad Laboratories Inc. Hercules, CA). The extracts were then aliquoted into individual tubes ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was developed by using ECL prime western blotting detection kit (Amersham) and images were captured using Versadoc (Biorad).
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bioRxiv - Cell Biology 2021Quote: ... and the RNA was reverse transcribed into cDNA using an iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). Quantitative PCR was performed with RT2 SYBR Green qPCR Master Mix (QIAGEN ...
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bioRxiv - Plant Biology 2021Quote: ... Up to 500 ng of RNA was used for reverse transcription using either the iScriptTM cDNA synthesis kit (Bio-Rad) or the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Plant Biology 2020Quote: ... the soybean AATRhg1 and GmRBOHC2 (Glyma.06G162300.1) ORFs were PCR-amplified from Williams82 cDNA generated by the iScript cDNA Synthesis Kit(Bio-Rad) and KAPA HiFi polymerase (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized from 1 µg DNA-free RNA using the iSCRIPT cDNA synthesis kit (BioRad, Hercules, CA, U.S.A). Real-time PCR master mixes were prepared using iQ sybr Green Mix (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... The purified total RNAs were converted to first-strand cDNAs by using a reverse transcription kit (Bio-Rad, Cat#1708891). The relative levels of HIV-1 un-spliced transcript and human MMP-2 mRNA were measured by qRT-PCR using the primers 5’AGGGACCTGAAAGCGAAAG3’ (HIV-1 un-spliced-forward ...
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bioRxiv - Genetics 2021Quote: ... RNA quantity and quality were determined on a NanoDrop 2000c (PeqLab) and cDNA was prepared using the iScript cDNA Synthesis Kit (BioRad). To quantify RNA expression ...
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bioRxiv - Microbiology 2021Quote: ... purified RNA (1000 ng) was converted to cDNA by using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s standard procedure ...
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bioRxiv - Microbiology 2021Quote: ... and relative transcript levels were measured for each sample in triplicate by quantitative qRT-PCR using SYBR Green PCR Kit (Biorad), on a MyiQ™ Real time PCR system (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... IL-10) responses were measured using a customized Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay kit (Biorad, USA) with the BioPlex 200 platform according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2021Quote: ... DNAse treatment were performed as in (Gigli-Bisceglia et al., 2018; Savatin et al., 2014) and cDNA synthetized with iScript cDNA Synthesis Kit (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: cDNA synthesis and quantitative reverse transcription PCR (qRT-PCR) were performed using the iTaq Universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) from extracted RNA was obtained using the iScript™ cDNA Synthesis Kit (Bio-rad, CAT No.:1708890) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... DNAase treatment and cDNA synthesis were done with the iSript gDNA clear cDNA synthesis kit (BIO-RAD, Hercules, CA, USA). Quantitative reverse transcription-polymerase chain reaction (RT-PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of total RNA was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Feldkirchen, Germany). Real-time PCR was performed using Platinum SYBR Green qPCR Super Mix-UDG (Life Technologies ...