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Citations for Bio-Rad :
3851 - 3900 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from 40 w1118 or de2f1b 80-85 hr AEL salivary glands using the Aurum Total RNA Mini Kit (Bio-Rad) with treatment of DNase I to remove genomic DNA contamination ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA and oligo (dT) primers were reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed as a two-step RT-qPCR by first synthesizing oligo(dT) primed cDNA using the iScript™ Select cDNA Synthesis Kit (Biorad), then running ddPCR via the standard EvaGreen program on a Biorad QX200 ddPCR system and analyzing in Quantasoft software (see Table S2 for primer sequences).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and TNF-α were quantified using real-time PCR and the SsoAdvanced Universal SYBR Green Supermix Kit (BIO-RAD, Hercules, CA). β-actin was used as an internal control ...
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (280 ng per sample) was used to generate cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). The cDNA was used as a template for qRT-PCR with SsoFast EvaGreen Supermix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μL of DNase-treated samples were reverse transcribed in a final volume of 10 μL using the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol and diluted 5-fold in nuclease-free water before qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume of BioID lysis buffer added was based on protein concentration determination using the DC Protein Assay Kit from Bio-Rad on the 10% aliquot samples lysed in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR expression analysis of selected candidates was performed using cDNAs produced from the iScript cDNA Synthesis Kit (Bio-Rad) in a LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was produced using the High Capacity RNA-to-cDNA Kit (Applied Biosystem) and the cDNA was pre-amplified with SsoAdvanced PreAmp Supermix (Bio-Rad) using primers from Integrated DNA Technologies or Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: Membrane and cytosolic proteins fractions from MS were extracted using ReadyPrep™ Protein Extraction Kit (Membrane I) (Bio-Rad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA samples were used in a qRT-PCR experiment employing the iQ SYBR Green Supermix Kit (Bio-Rad, Munich, Germany). PCR was conducted in a CFX96 RT-PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: Agarose plug embedded nuclei were Proteinase K treated for two days followed by RNAse treatment (Biorad CHEF Genomic DNA Plug Kit). DNA was recovered from agarose plugs according to IrysPrep™ Plug Lysis Long DNA Isolation guidelines (BioNano Genomics) ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Concentration of protein lysates was determined using either the Pierce BCA protein assay kit (Pierce) or the DC Protein Assay (Bio-Rad) following the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... An amount of 500-1000 ng of purified total RNA was then subjected to reverse transcription using the iScript cDNA synthesis kit (Bio-Rad), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 800 ng of purified RNA was used as a template to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). End-point PCR was then conducted using primer sets EH13/EH14 and EH1/EH2 ...
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bioRxiv - Neuroscience 2019Quote: ... total protein concentration of the supernatant was assayed by using Bio-Rad protein assay kit (Bio-Rad, Marnes-la-Coquette, France). Equal amounts of each protein sample were separated on NuPAGE Bis-Tris or Tris-Acetate gels and transferred to nitrocellulose or PVDF membranes ...
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bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
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bioRxiv - Physiology 2020Quote: ... Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules, CA). Antibodies against ERα (F-10 mouse – for WB) ...
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Quantitative real-time (qRT)-PCR was performed using the iCycler-iQ system and the iQ SYBR Green Supermix Kit from Bio-Rad. The primer pairs used to amplify the target genes were designed using the Primer3 software (http://bioinfo.ut.ee/primer3/ ...
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bioRxiv - Plant Biology 2019Quote: ... and the Takyon No ROX SYBR 2X MasterMix blue dTTP qPCR Kit (both Eurogentech) for quantitative real time PCR using a CFX96 detection system (Bio-Rad). The previously described reference genes Protein Phosphatase 2A (PP2A ...
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bioRxiv - Cell Biology 2019Quote: ... RNA concentration and quality were determined using Nanodrop (PeqLab) and cDNA was synthesized with the iScript cDNA synthesis kit (Bio-Rad) following manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: Total RNA from SIM-A9 cells with varying treatment conditions was reverse transcribed to cDNA libraries using the iScript cDNA Synthesis Kit (Bio-Rad) with a 1 μg RNA input ...
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bioRxiv - Physiology 2019Quote: ... The cDNA synthesis was performed with 1μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2021Quote: ... The HRP conjugated secondary antibodies were visualized by using an enhanced chemiluminescence detection kit and ChemiDoc Touch Imaging System (Image Lab, Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNase-treated RNA was used to perform cDNA synthesis in a final volume of 20 μl using the iScript cDNA synthesis kit (Biorad, USA). The reaction was performed at 46°C for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The chemiluminescence of the membranes was developed using Super Signal Femto Substrate Kit (Pierce) and developed using ChemiDoc Touch Imager (Bio-Rad). Primary antibodies and conditions used are as follows ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed using the TaqMan Fast Advanced Cells-to-CT kit with the FX96 Real-Time PCR Detection System (Bio-Rad) with the TaqMan probes listed in table S15 ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK). Spots were enumerated using an AID ELISpot reader and software (AID).
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bioRxiv - Systems Biology 2021Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of purified RNA were used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, Irvine, CA), and real-time quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2021Quote: ... A preamplification step that amplifies the amount of cDNA available for downstream RT-qPCR analysis was conducted using SsoAdvanced PreAmp Kit (BioRad, 1725160). RT-qPCR was performed using TaqMan Gene expression assays (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were boiled for 10 min and quantified with the DC protein assay kit following the microplate protocol provided by the company (Bio-Rad). Whole cell lysates (15-40μg protein ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined using the BioRad DC microplate assay protocol (BioRad DC Protein Assay Kit, BioRad, Hercules, California #5000112). Protein Standards and samples were run on NuPAGE 10% Bis-Tris Gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... N2a cells were harvested at the same time points for reverse-transcriptase (RT) PCR generation using iScript cDNA Synthesis kit (Bio-Rad), and quantitative PCR (QPCR ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and measured as detailed above and then converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad #1708891). Quantitative RT-PCR was carried out with TaqMan Gene Expression Assay reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.