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Citations for Bio-Rad :
3451 - 3500 of 4485 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from total RNA (0.1 – 1.0 µg) with iScript Advanced cDNA Synthesis Kit (BioRad, Hercules, CA, USA). QRT-PCR was performed using PowerUp SYBR Green Master Mix reagent (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... The extracted RNA was amplified by iTaq universal SYBR Green one-step PCR kit (Bio-Rad, Hercules, CA, USA) and 10μM primers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: The qPCR mixture of 10 μl was prepared using the iQ™ SYBR® Green PCR Kit (Bio-Rad): 5 μl of 2x iQ™ SYBR® Green Master Mix ...
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bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted and reverse-transcribed according to the manufacturer’s protocol (iScript™ cDNA Synthesis Kit, BIO-RAD). cDNA was loaded in duplicate and qPCRs were performed using the BIO-RAD CFX System (BIO-RAD ...
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bioRxiv - Genomics 2021Quote: The single nucleus ATAC-seq experiment was performed using the SureCell ATAC-Seq Library Prep Kit (Bio-Rad, 17004620) following the manufacturer’s protocol for the OMNI-ATAC version (45) ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... The protein lysate concentration was quantified using Pierce BCA Protein Assay Kit (ThermoScientific) and10μg of soluble protein lysates were separated on a 4-12% Bis-Tris gel (BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... 1μg of purified RNA was used to make cDNA using the iScript™ cDNA synthesis kit (Bio-Rad, USA) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... or 15% SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45μM LF PVDF Transfer Kit (Bio-Rad). Membranes were incubated for 1h at room temperature with blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestion of genomic DNA was carried out using Turbo DNase treatment (TURBO DNA-free™ Kit, Bio-Rad, USA) per the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Physiology 2021Quote: ... Same amount of total RNA was used for reverse transcription by using iScript™ cDNA Synthesis Kit (Bio-Rad). RT-qPCR was done in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... The cDNA for each sample was reverse-transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA) from 1 □g of input RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA synthesis kit (BIORAD, CAT#1708891). Quantitative real-time PCR reactions were set up in technical triplicates with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... One microgram of extracted RNA was reverse transcribed into cDNA using iScript cDNA Synthesis Kit (BIO-RAD, Cat #1708890). Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward ...
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bioRxiv - Cell Biology 2021Quote: ... each sample was used for the cDNA synthesis carried out by the iScript cDNA synthesis kit (BioRAD, CA, USA). The real-time PCR reactions were performed by the KAPA SYBR FAST qPCR Master Mix Kit (KAPABIOSYSTEMS ...
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bioRxiv - Cancer Biology 2021Quote: ... The RNA samples (1μg) were thereafter reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, 1708890) according to the accompanying instructions ...
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bioRxiv - Microbiology 2021Quote: ... the RNA was transcribed into cDNA using the iScript™ Select cDNA Synthesis Kit with random primers (Biorad, Germany). The amount of speC transcripts was quantified using primers 29 and 30 (Tab ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were developed using Chemiluminescent Substrate kit (Pierce) and visualized using ChemiDoc XRS+ system (Bio-Rad, Hercules, CA, USA). All densitometric bands were quantified using ImageJ (NIH).
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bioRxiv - Immunology 2021Quote: ... 100ng-1μg RNA was used to synthesize cDNA by reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad). Quantitative PCR reactions were run with cDNA template in the presence of 0.625μM forward and reverse primer and 1x solution of iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Immunology 2020Quote: cDNA was synthesized from the indicated tissue RNA using iScript cDNA synthesis kit (#170-8891) acquired from Bio-Rad according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2020Quote: Cytokine concentrations in the serum of mice were determined using a Bio-Plex Pro assay kit (Bio-Rad Laboratories). A total of 50 μl of antibody-conjugated beads was added to the assay plate ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysate was spun down at 14000 rpm and protein concentration was determined using the Bradford assay as detailed in the manufacturer’s kit (Biorad). Samples were denatured with SDS and methylene blue ...
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bioRxiv - Cell Biology 2021Quote: ... and total cellular protein content was determined using the DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA). Then ...
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bioRxiv - Genomics 2021Quote: ... Protein concentrations of the lysates were determined using a Bio-Rad DC protein assay kit (Bio-Rad, catalog # 5000111), the lysates were denatured in the SDS-PAGE loading buffer at 100°C for 10 min ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... 500 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit according to manufacturer instructions (BioRad). Primers used for RT-qPCR are shown in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of RNA were converted into cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA; 1708891) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR analysis was performed using the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed to cDNA using the iScript™ cDNA Synthesis kit (Bio-Rad #1708890, Hercules, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated from total RNA via reverse transcription reaction using an iScript RNA-to-cDNA kit according to the manufacturer’s instructions (BioRad). cDNA was then amplified using the iQ SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... RNA templates were then utilized for complimentary DNA (cDNA) synthesis utilizing the iScript cDNA synthesis kit protocol (Bio-Rad).
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bioRxiv - Genomics 2022Quote: ... 0.5 μg of purified RNA was reverse transcribed using either the iScript gDNA Clear cDNA Synthesis Kit (BioRad, #1725034) or Maxima H Minus First Stand cDNA Synthesis Kit (Thermo Fisher Scientific M1681) ...
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bioRxiv - Physiology 2022Quote: ... and the first-strand cDNA was prepared using the iScriptTM cDNA synthesis kit (Bio-Rad, #1708890, Hercules, CA, USA). qPCR analysis was performed with SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants were used for protein quantification via the use of the DC(tm) Protein Assay Kit (Bio-Rad). Equal loading of proteins (10–20 µg ...
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bioRxiv - Plant Biology 2022Quote: One microgram of RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative real-time PCR analysis was performed on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... One-step RT-qPCR was performed using an iTaq Universal SYBR Green One-Step RT-qPCR Kit (BIO-RAD). The reaction was performed in a total volume of 20 μL by mixing 10 μL iTaq universal SYBR Green reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was analyzed by TaqMan qRT-PCR one-step RT-PCR kit for probes (Bio-Rad laboratories, Italy) with a TaqMan RT-PCR instrument (CFX384 real time system ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 μ of total RNA using iScript gDNA clear cDNA synthesis kit (Bio-Rad, 1725035) with random primers ...
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bioRxiv - Microbiology 2023Quote: ... was used to purify RNA followed by cDNA synthesis (0.5 μg of RNA) using iScript RT kit (Bio-Rad) and qPCR was performed using the Brilliant II SYBR® Green QPCR master mix (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were blotted onto a nitrocellulose membrane using the Trans-Blot Turbo TRA Transfer Kit (Bio-Rad, Feldkirchen, Germany). Following protein transfer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunoblot was performed by transferring protein to a PVDF membrane using Trans-Blot Turbo RTA Transfer Kit (Biorad, 1704272) and the Biorad Transblot Turbo System ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was then incubated in a transfer buffer from the trans-blot turbo RTA transfer kit (Biorad, 1704272) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... at 100 V and then transferred using the Trans-Blot Turbo transfer kit onto nitrocellulose membranes (Bio-Rad, #1704270). Membranes were blocked with 5% nonfat dry milk prepared in TBST (Tris-buffered saline with Tween 20 ...
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bioRxiv - Immunology 2023Quote: ... and 500 ng of total RNA was reverse transcribed using the iScript™ gDNA Clear cDNA Synthesis Kit (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized using oligo(dT)/random primer mediated reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad) with 1 μg of DNA-free total RNA as input ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...