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Citations for Bio-Rad :
9001 - 9050 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Both total and mitochondrial protein concentrations were determined using the Bradford method (Bio-Rad protein assay, Bio-Rad Laboratories GmbH, München, Germany). Protein extract volumes equivalent to 50–75 μg of total protein were concentrated into smaller volumes by lyophilizing the samples with a Savant Speed Vac Plus SC210A centrifugal evaporator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... TNF-β were detected by using the premixed 48-plex Bio-Plex Pro Human Cytokine Assay (Bio-Rad, Hercules, CA, USA; 12007283) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene and microRNA expression were quantified with TaqMan Expression assays on a CFX96 Touch Real-Time System thermocycler (Bio-Rad, Hercules, CA). Gene expression assays were normalized to the expression of RPS9 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was transferred to a new tube and total protein concentration was determined by Bradford protein assay (BioRad, C500-0205). For each condition ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Immunology 2021Quote: ... The expression of targeted genes and GAPDH were determined using Taqman Gene Expression assays and CFX96 Touch Real-Time System (BioRad, Hercules, CA). Relative expression values were determined by the 2-ΔCt method where samples were normalized to GAPDH expression as described previously (52).
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bioRxiv - Microbiology 2022Quote: ... The reaction rate and total protein concentration (determined by the method of Bradford with the BioRad Protein Assay reagent; BioRad, Hercules, CA) were used to determine CAT activity ...
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bioRxiv - Molecular Biology 2023Quote: ... The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (28) ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected in a 1.5 mL microtube and 10 µL of the suspension was used for live cell counting using the trypan blue exclusion assay with a TC20™ automated cell counter (Bio-Rad). BSA (0.05 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was kept and the total protein concentration was determined by standard Bradford method (Bio-Rad Protein Assay Dye Reagent #5000006) using a BSA calibration curve (MP BIomedicals #160069 ...
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bioRxiv - Microbiology 2023Quote: ... intrahepatic cccDNA values were normalized by the cell number measured by the hRPP30 copy number variation assay (Bio-Rad, Pleasanton, California, USA)[53] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by SARS-CoV-2 qRT-PCR using the Seegene Allplex™ 2019-nCoV Assay (cat. no: RP10243X) on a CFX96 Real-time PCR Detection System-IVD (Bio-Rad)
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: Maternal plasma and SI IEL proteins were assayed for cytokine levels according to manufacturer’s instructions using the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad, Mississauga, Canada) and the Luminex system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... intrahepatic cccDNA values were normalized by the cell number measured by the hRPP30 copy number variation assay (Bio-Rad, Pleasanton, California, USA)68 ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, California, USA) and concentrated to approximately 100-200 μM.
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Plant Biology 2024Quote: ... The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad; www.bio-rad.com) and bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The thermal shift assay was performed by following methods previously described,39 in a 96-well clear low-profile plate (Bio-Rad, #MLL9601) using CFX96 touch Real-Time PCR detection system ...
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bioRxiv - Immunology 2022Quote: ... aliquots of frozen mEVs were thawed at 37°C and Bio-Rad Protein Assay (Bradford 1976) was performed following manufacturer’s instructions (500-0006 Bio-Rad. München, Germany). Samples were diluted with filtered PBS 1:50 and bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration of the supernatant was determined using the BCA protein assay (Pierce™) and 15 µg was loaded on the gel (3450124, Bio-rad), which was next turbo transferred on nitrocellulose membrane (1704159 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions pooled and the concentrations of conjugated mi3 particles were determined using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA). Conjugated nanoparticles were characterized by electron microscopy imaging and SEC as shown in Figure S1C-E ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were assessed using Bio-Rad BioPlex assays in both plasma and PFC homogenates (Th1/Th2 rat cytokines Bio-Rad, #171k1002M), according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... using gene specific primers purchased from Fluidigm (DELTAgene Assay) and detected using SsoFast™ EvaGreen Supermix with Low ROX (Bio-Rad Laboratories). Results from these assays were normalized based on the values for GAPDH mRNA.
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bioRxiv - Cell Biology 2023Quote: ... The supernatants of the cell lysates were normalized for total protein concentration according to Bradford assay (#5000006, Bio-Rad, Hercules, CA, USA), mixed with SDS loading dye ...
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bioRxiv - Biochemistry 2023Quote: ... The culture medium after incubation was analyzed for the presence of secreted human cytokines using Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, USA) and Bio-Plex 200 Systems (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... ATP contents were expressed in nmol/mg protein (using the Bio-Rad Protein Assay Cat. #500-0006, Bio-Rad, Hercules, California, USA).
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bioRxiv - Cancer Biology 2023Quote: ... RNA from the FFPE clinical samples were processed through BioRad mRNA ddPCR fusion assays for RET (ID dHsaEXD81378442, Bio-Rad, Hercules, CA), ROS1 (ID dHsaEXD73338942 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were cleared by centrifugation (16000 g, 15 min) and protein concentrations determined by protein assay with Bradford dye reagent (Bio-Rad, 5000006). Proteins separated by SDS-PAGE without boiling due to the heat sensitivity of WDR62 [7] ...
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bioRxiv - Biophysics 2024Quote: ... we collected the supernatant in a new 1.5 ml reaction tube and determined the protein concentration of each sample by a Bradford protein assay (Biorad, Cat. No.: 5000006). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... The concentration was determined by a Bradford assay and the purity was estimated by SDS-polyacrylamide gel electrophoresis (PAGE) using Image Lab 6.1 software (2022, Bio-Rad Laboratories, Inc.). This expression and purification procedure was repeated for TmrA.
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bioRxiv - Molecular Biology 2024Quote: ... The size and purity of purified proteins were monitored by SDS-PAGE and protein concentrations were determined by the RC DC Protein Assay (Bio-Rad, 5000122).
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR assays were conducted on Bio-Rad CFX Manager v3.1 using the iTaq™ Universal SYBR® Green Supermix (Bio-rad, Hercules ...
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bioRxiv - Microbiology 2024Quote: ... MVs were suspended in 100 µl of phosphate buffered saline (PBS) and quantified using i) a Bradford assay (Bio-Rad 500-0006) and a bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was transferred to a clean tube and total protein concentration was measured with the DC protein assay (Bio-Rad #500-0112). 4X Laemmli Sample buffer (Bio-Rad #1610747 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants were collected and stored at −80°C for subsequent analysis of total protein content using a bicinchoninic acid (BCA) assay (Bio-rad, # 5000001). Following the lavage ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble lysate was normalized to 30 µg protein lysate in 15 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006). Proteins were then reduced and alkylated by the addition of 10x tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Cancer Biology 2024Quote: ... WCL were cleared by the centrifugation at 14,000 RPM for 15 min at 40C and protein concentration was determined using the Bradford assay (Bio-Rad Laboratories, 5000006).
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bioRxiv - Cell Biology 2020Quote: ... The membranes were treated with ECL chemiluminescence detection reagent (GE healthcare, product # RPN2106V1 and RPN2106V20 and imaged using the Bio-Rad Chemidoc system (Bio-Rad Laboratories). Densitometry was done using ImageLab software (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... samples were transferred onto Whatman® Westran® PVDF membrane 0.45 μm using the Bio-Rad Mini Trans-Blot® Wet Transfer System (Bio-Rad, Hercules, CA, USA), for 60 min at 100V in a Wet Transfer system ...