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Citations for Bio-Rad :
8851 - 8900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... IL-28A/IFN-λ2 and IL-29/IFN-λ1) using a Bio-Plex ProTM Human Inflammation Panel 1 Express assay (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purity was analyzed by SDS-PAGE and the protein concentration was determined using the Bradford assay (QuickStartTM, Bio-Rad, Germany) with BSA as the protein standard (Zor & Selinger ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR assays were conducted using gene specific primers (Supplementary table X. Primer list) using SYBR green master mix (Bio-Rad). The reaction was run for a total of 40 cycles ...
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bioRxiv - Microbiology 2024Quote: ... The copy number of stx2 in each cellular DNA sample was determined by droplet digital PCR using the EvaGreen assay (Bio-Rad) with the stx2-specific primers described above ...
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bioRxiv - Biochemistry 2024Quote: Thermal unfolding of GCase was followed by thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad)48 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was used to quantify the total protein concentration in the sample by using the DC protein assay (Bio-Rad) and a BSA standard curve [54] ...
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bioRxiv - Immunology 2022Quote: ... the beads were re-suspended in assay buffer and the plates were read on a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein amount in each lysate sample was measured using a DC™ protein assay (Bio-Rad, Cat#5000113, #5000114, #5000115). Caspase-3 activity in each sample was calculated as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of extracts (quantified using Lowry assay) were subjected to SDS-PAGE analysis on 4-15% Criterion TX gels (Bio-Rad), transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Two microliters of each RNA sample were amplified in duplicate assays with a CFX detection system (Bio-Rad, Hercules, CA, USA) using TaqMan RT-PCR master mix (Quanta Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... cleared supernatant was transferred to the new clean Eppendorf tubes and total protein concentration was measured using Bradford assay by Bio-Rad Protein Assay kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The post-nuclear fraction was collected and protein concentration was determined by the Bradford protein assay (Bio-Rad Laboratories, Hercules, USA) using bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... The qRT-PCR assay was performed using ChamQ SYBR Master Mix (Vazyme, Q311-02) on Bio-rad CFX96 real-time PCR system (Biorad, USA). All primers used in the present study were listed as following ...
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bioRxiv - Cell Biology 2023Quote: ... gene expression of tdTomato [TaqMan probe FAM - Mr07319439_mr (Thermofisher)] and GAPDH (Rufino-ramos et al. 2022)(Al Ali et al. 2021) was analyzed using ddPCR following the PrimePCR ddPCR Gene Expression Probe Assay (Bio-Rad). Using the manufacturer’s protocol droplets were generated with DG8 Cartridge using a QX200 droplet generator and PCR was performed with thermal cycling conditions using QX200 Droplet Reader and QuantaSoft Software (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Microbiology 2023Quote: Cytokine and chemokine levels were measured in mouse serum using the Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad). Cytokines and chemokines were recorded on a MAGPIX machine (Luminex ...
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bioRxiv - Immunology 2023Quote: ... the supernatant containing extracted soluble protein was collected and protein concentration was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (5000006, Bio-Rad) according to the standard protocol provided by the manufacturer.
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bioRxiv - Immunology 2023Quote: ... PrimePCRTM PCR Primers assay plates were custom made with validated primers for use with EvaGreen dye-based chemistry (Bio-RAD, USA). Samples were run on CFX96 Touch Real-Time Detection System (Bio-RAD ...
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bioRxiv - Physiology 2023Quote: ... The resulting lysates were used for quantification of the total protein content with the DC protein assay (Bio-Rad, Hercules, USA) according to Lowry et al ...
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bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Bioengineering 2023Quote: ... followed by an additional wash and resuspension in Assay Buffer for readout by high-throughput cytometry (Bio-Rad ZE5 cell analyzer). Results were decoded using Nomic software ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μg aliquots of mitochondria were flash-frozen in liquid nitrogen after measuring the protein concentration by a Bradford assay (Bio-Rad) and stored at −80°C.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR assay was carried out using gene-specific fluorescently labelled probes and using CFX96 PCR system by Bio-Rad. The nucleotide sequences used for real-time qPCR analysis are showed in Table 1 ...
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bioRxiv - Bioengineering 2023Quote: ... Assay temperature kinetics study was performed in PCR tubes at 20 μL volume with the same assay concentrations as discussed above in a CFX Connect Real-Time System (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from beads using 400 mM Imidazole and their concentrations were determined using a Bradford Assay (BioRad, Hercules, CA) with BSA standard ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of extracted protein was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006) following manufacturer’ s protocol.
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
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bioRxiv - Plant Biology 2024Quote: The results of RNA-seq were validated by quantitative real-time PCR (qRT-PCR) assay of selected genes using a CFX96 Touch Real-Time PCR system (BioRad, USA) as described previously20.
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bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the Bio-Rad protein assay according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA). The proteins were then separated on a 10% polyacrylamide-SDS gel (90 µg of protein/lane ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracted from the samples were quantified using RC DC Protein Assay Reagents Package (Bio-Rad Laboratories Ltd, Cat. No. 5000120,).
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bioRxiv - Microbiology 2024Quote: ... the beads were re-suspended in assay buffer and the plates read on a Bio-Plex 3D Suspension Array System (Bio-Rad).
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Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damagebioRxiv - Microbiology 2024Quote: Telomere length of placental DNA was measured using an Absolute Telomere Length (aTL) assay based on qPCR technique (using primers from IDT and SYBR Green from Bio-Rad), as described previously.15
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bioRxiv - Immunology 2024Quote: Primary T cell culture supernatants were analyzed for cytokine content six days after activation with Bio-Plex Pro™ Human Cytokine 27-Plex Assay (#M500KCAF0Y, BioRad) according to the manufacturer’s protocol.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA samples were screened for vertebrate DNA using vertebrate-specific qPCR assay targeting a fragment of the 16s rRNA on a CFX Duet PCR system (Bio-Rad) [16] ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture supernatants from 72hr direct co-cultures were diluted 1:2 and used to determine the expression of 23 soluble proteins with the Bio-Plex Pro Human Cytokine 17-plex Assay (Biorad #M5000031YV) and a custom 6-plex Luminex array (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... or the RNeasy Micro Kit (Quiagen) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). Quantitative realtime PCR was performed using the GoTaq SYBR Green qPCR Master Mix (Promega ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was produced using a cDNA Synthesis kit (iScript™ cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA). The diluted cDNA was used for real-time PCR quantification of mRNA using iTaq SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 500ng of RNA was reverse transcribed using the reverse transcription kit (iScript cDNA synthesis kit, Biorad, Cat# 1708891). The resultant cDNA was diluted ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-solubilized proteins (0.2-0.3 OD600 cell equivalents) were resolved by 10% SDS-PAGE prior to transfer to nitrocellulose membranes using the Trans-Blot Turbo™ system (Bio-Rad Laboratories, Hercules, CA) and blocked with Western Blocking Reagent (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed in a Bio-Rad Criterion TM Vertical Electrophoresis Cell at 90-120V for 1-2 hours and then transferred to a PVDF Transfer Membrane (#88518, Bio-Rad, Hercules, CA, United States) using the Bio-Rad Trans-Blot Turbo Transfer System ...
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bioRxiv - Biochemistry 2021Quote: ... bands on the membrane were visualized using Chemi-Lumi One Ultra (NACALAI TESQUE, INC., Japan) and a ChemiDocTM Imaging System (Bio-Rad Laboratories, Inc., CA, USA). Signal intensity was quantified using ImageLab software (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (GE-Healthcare, Chicago, IL, USA) for 1 hour at 15V using the Trans-Blot semi-dry transfer system (Bio-Rad Laboratories, Hercules, CA, USA). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... proteins within the gel were partially transferred to polyvinylidene difluoride (PVDF) membrane (250 mA current flow, 1.5 h) using wet transfer apparatus (Bio-Rad Laboratories Inc., Hercules, CA, USA). Blocking of the PVDF membranes were done for overnight at 4°C temperature in shaking condition using blocking buffer (0.019 M Tris ...
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bioRxiv - Neuroscience 2021Quote: ... and half of each biotinylated fraction were separated into 10% SDS-PAGE gels electrophoresis and transferred to nitrocellulose membranes (Bio-Rad Laboratory, Hercules, CA, USA). Subsequently ...