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Citations for Bio-Rad :
7501 - 7550 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was performed at 100 V for ∼60 minutes and proteins transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad) which utilizes Trans-Blot Turbo Midi PVDF transfer packs (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated on a 10% SDS-PAGE and transferred to 0.2 µm nitrocellulose membrane using the Trans-blot SD semi-dry transfer cell (Bio-Rad). Blots were blocked for one hour with Odyssey Blocking Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then transferred to a Nytran SuPerCharge (Cytiva) nylon membrane using a Bio-Rad Trans-Blot Turbo (Bio-Rad) at 200mA for 60 minutes ...
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bioRxiv - Microbiology 2024Quote: ... separated by SDS-PAGE on a 10% acrylamide gel and transferred onto nitrocellulose membrane using a Mini-Transblot system (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE using homemade 10% polyacrylamide gels and semi-dry transferred to a 0.2 µm nitrocellulose membrane using the Trans-Blot Turbo Transfer System (BioRad, #1704270). Membranes were blocked using intercept blocking solution (Licor Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Gels were transferred onto polyvinylidene fluoride membrane using a semi-dry transfer apparatus (Trans-Blot Turbo Transfer System, Bio-Rad) and were blocked for 45 minutes in 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed three times in TBS/0.05% TritonX-100 and developed using Clarity Western ECL Substrate (Bio-Rad, 1705061). Blots were imaged on an Amersham Imager 680 ...
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bioRxiv - Microbiology 2024Quote: ... membrane for 30 min at 2.5 V and 25 mA using a semi-dry Trans-Blot Turbo system (Bio-Rad). Membranes were blocked by incubating them for two hours at room temperature (RT ...
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bioRxiv - Microbiology 2024Quote: ... DD-HA-BFD2Δeif1.2 were subjected to electrophoresis using 10% polyacrylamide SDS-PAGE gels and transferred onto 0.45-µm nitrocellulose membranes (Bio-Rad) with wet/tank transfer system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysates were applied to a cellulose acetate membrane with a pore size of 0.2 μm (Advantec Toyo, Tokyo, Japan) using a Bio-Dot SF blotter (Bio-Rad). The membrane was blocked in PBS-T buffer containing 5% skim milk for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a nitrocellulose membrane with a Trans-Blot®Turbo™ Transfer Starter System (BioRad, 170-4155). For high molecular weight samples (SYNJ1-BirA* ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were loaded onto 10% bis-Tris gels and transferred to Immun-Blot PVDF membranes (1620177, Bio-Rad, Hercules, CA), which were then blocked in 5% blocking grade skim milk (170–6404 ...
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bioRxiv - Plant Biology 2024Quote: ... was run at 120 V for 1:30 h and blotted to polyvinylidene fluoride (PVDF) membrane (Hybond P 0.45, Amersham, Cytiva, Marlborough, USA) in a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) for 1.15 h with 1.5 mA/cm2 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 µL per sample was separated on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Pack (Bio-Rad) for 10 min at 25 V ...
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bioRxiv - Molecular Biology 2024Quote: ... 20% SDS and transferred using Transfer Buffer (Running Buffer, 20% Methanol) for 1 h and 30’ on Nitrocellulose membrane (BioRad). Membranes were blocked in 5 % milk ((W/V ...
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bioRxiv - Biochemistry 2024Quote: Proteins were separated by SDS-PAGE using 10% polyacrylamide gels and transferred electrophoretically to nitrocellulose membranes (Bio-Rad, Ref 1704271). Membranes were incubated in blocking buffer (Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (0.2 μm, Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) in transfer buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... with XT MES buffer and was transferred to polyvinylidene difluoride membranes using the Trans-Blot Turbo Transfer system (Bio-Rad). Membranes were blocked in 5% milk for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts were resolved on 18% SDS-PAGE and proteins were transferred onto the 0.45µm nitrocellulose membrane (Bio-Rad, 1620115). The blots were blocked by using 2.5 % bovine serum albumin (Himedia ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred using a Trans-blot semi-dry system and Trans-blot Turbo Midi 0.2 µm PVDF pre-cut membranes (Bio-Rad). Membranes were blocked in either PBS + 0.5% Tween-20 (PBST ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred onto 0.2 µm polyvinylidene difluoride membranes (PDVF) via turboblot transfer (Trans-Blot Turbo Mini PVDF Transfer Packs, Bio-rad), at 2.5A ...
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bioRxiv - Neuroscience 2024Quote: ... For preparation of western bot: eluted proteins were separated by SDS-PAGE and subsequent transfer to nitrocellulose membrane (Bio-Rad). Membranes were then blocked in tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... Samples were analyzed in 6 % Tris-glycine SDS-polyacrylamide gels, transferred on nitrocellulose membranes (Protran 0.45 μm, Amersham) using a semi-dry cell (Trans-Blot SD, Bio-Rad) in transfer buffer (39 mM glycine ...
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bioRxiv - Neuroscience 2024Quote: ... were electrophoresed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and transferred proteins from gel onto polyvinylidene difluoride fluoride (PVDF) membranes (BioRad). After transformation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated with appropriate primary antibodies in Intercept blocking buffer supplemented with 0.1% TWEEN 20 (Bio-Rad, 927-70001). Membranes were washed 3 times with PBS supplemented with 0.1% TWEEN 20 and incubated with secondary antibodies for 2 h at 25 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were separated on SDS-polyacrylamide gels and transferred to ClearTrans SP PVDF Membranes (Fujifilm Wako Pure Chemical) using a Trans-Blot Turbo Transfer System (BIORAD). After blocking with 3% skim milk or 5% BSA (for anti-phospho-S6K and anti-phospho-ERK1/2 ...
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bioRxiv - Immunology 2024Quote: ... the protein signals in the membrane were visualized and recorded using SuperSignal West Pico PLUS Chemiluminescent Substrate by Bio-Rad chemiluminescence system ...
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bioRxiv - Immunology 2024Quote: ... Membranes were then blocked with 5% non-fat milk for one hour at room temperature (Bio-Rad, Hercules, CA, USA). After blocking ...
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bioRxiv - Immunology 2024Quote: ... the SDS-PAGE gels were transferred to an Amersham Hybond P Low fluorescence 0.2 μm PVDF membrane (Cytiva) using the Trans-blot SD semidry transfer cell (Bio-Rad) (25 V ...
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bioRxiv - Plant Biology 2024Quote: ... The extracted samples were resolved on 10% (w/v) SDS-PAGE gels and were subsequently transferred onto PVDF membranes with the TransBlot (Biorad). The membrane was probed with 1:10000 dilution of either anti-FLAG (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto 0.45 um nitrocellulose membranes at 300 mA for 1.5 hr in a Criterion blotter (Bio-Rad, USA). After 1 hr blocking at room temperature in TBST (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred on nitrocellulose membrane at 100V for 2 h using the Mini trans-blot cell (Bio-Rad, #1703930). Membranes were blocked with 5% skim milk diluted in PBST (PBS + 0.1% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... proteins were transferred to 0.2 µm nitrocellulose membranes by semi-dry electrotransfer in a Transblot turbo transfer system using recommended standard settings (Bio-Rad). Membranes were blocked with 5% w/v non-fat milk powder in PBS for a minimum of 3 h with continuous rocking ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a methanol-activated PVDF membrane (Cytiva) using a Mini Trans-Blot Transfer Cell (Bio-Rad) at a constant 200 mA and 4 °C for 2.5 hours in cold transfer buffer ...
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bioRxiv - Cell Biology 2024Quote: ... was followed by transfer at 66 mV for 120 minutes onto Immun-Blot Low Fluorescence PVDF Membrane (1620264, Bio-Rad). Membranes were blocked using Intercept Blocking Buffer (927-70003 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Amersham Hybond PVDF, #10600023 – 0.45 µm) using a wet transfer system (Bio-Rad) at constant 80V for 2 h (1x transfer buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto PVDF membranes (Amersham Hybond PVDF, #10600023 – 0.45 µM (300mm)) using a wet transfer system (Bio-Rad) at constant 80V for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to nitrocellulose membranes (Amersham Protran 0.45 NC, Cytiva) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Primary antibodies were ...
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bioRxiv - Cell Biology 2024Quote: ... The separated proteins were then transferred to Trans-Blot Turbo Midi 0.2 μm PVDF membranes (1704157, Bio-rad, the U.S.A) using the Trans-Blot Turbo System (2,5 A ...
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bioRxiv - Cancer Biology 2024Quote: ... Around 30 μg of protein lysate per sample was resolved by SDS-PAGE and transferred to a PVDF membrane with the Semi-Dry and Rapid Blotting System according to the manufacturer’s protocol (Bio-Rad). The membrane was blocked in 5% BSA and incubated with antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins (10-15 μg) were resolved on 4-15% SDS-PAGE gels and transferred onto nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked in Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction products were then resolved by SDS–PAGE on a 4–20% gradient gel and transferred to a nitrocellulose membrane (BioRad). The membrane was blocked with 2.5 % BSA in TBST for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Biochemistry 2024Quote: ... in SDS/Tris-glycine running buffer and transferred to membranes using the semi-dry trans-Blot Turbo transfer system (Biorad). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor cat ...
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bioRxiv - Cell Biology 2024Quote: For detection of protein band by WB protein bands were transferred from polyacrylamide gel to polyvinylidene fluoride (PVDF) membrane using a TransBlot Turbo device (BioRad). PVDF membrane was blocked for 10 min with 10% blocking milk (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: Protein extracts were subjected to SDS-PAGE and transferred to a nitrocellulose membrane by wet western blot transfer (Bio-Rad). The membrane was blocked in TBST buffer (TBS + 1% Tween ...
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bioRxiv - Cell Biology 2024Quote: ... and 5-10 μg aliquots were fractionated by SDS-PAGE and then transferred to nitrocellulose 0.45 μm membrane (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% BSA in 1x Tris-buffered saline + 0.1% Tween 20 (TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated with ECL TM Prime Western Blotting Detection Reagent (Cytiva) and images acquired using ChemiDoc Touch System (BioRad) within the linear range.
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled in SDS loading buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.45 µm nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2024Quote: SDS-PAGE gels were transferred to methanol-activated PVDF membrane by Trans-Blot SD semi-dry transfer at a constant voltage of 10V for 1 hour (BioRad). Membranes were washed with TBST before blocking in 5% milk (TBST ...
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bioRxiv - Cell Biology 2024Quote: ... They were transferred to nitrocellulose membranes at 25 V for 30 mins in the Trans-Blot Turbo system (Bio-Rad).